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Literature summary extracted from

  • Park, C.S.; Yeom, S.J.; Lim, Y.R.; Kim, Y.S.; Oh, D.K.
    Characterization of a recombinant thermostable L-rhamnose isomerase from Thermotoga maritima ATCC 43589 and its application in the production of L-lyxose and L-mannose (2010), Biotechnol. Lett., 32, 1947-1953.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.1.14 expressed in Escherichia coli ER2566 cells Thermotoga maritima

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.14 37
-
L-rhamnose purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0) Thermotoga maritima
5.3.1.14 69
-
L-Lyxose purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0) Thermotoga maritima
5.3.1.14 76
-
L-Mannose purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0) Thermotoga maritima
5.3.1.14 111
-
L-xylulose purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0) Thermotoga maritima
5.3.1.14 123
-
L-Fructose purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0) Thermotoga maritima

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.3.1.14 Mn2+ enzyme activity is the highest with Mn2+, the optimal Mn2+ concentration is 1 mM (6.7fold increase activity relative to no treatment) Thermotoga maritima

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.1.14 45527
-
4 * 45527, calculated from amino acid sequence Thermotoga maritima
5.3.1.14 46000
-
4 * 46000, SDS-PAGE Thermotoga maritima
5.3.1.14 184000
-
gel filtration Thermotoga maritima

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.14 Thermotoga maritima
-
-
-
5.3.1.14 Thermotoga maritima ATCC 43589
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.1.14 His-Trap column chromatography Thermotoga maritima

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.3.1.14 0.5
-
using D-gulose as substrate, purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0) Thermotoga maritima
5.3.1.14 1.1
-
using D-psicose as substrate, purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0) Thermotoga maritima
5.3.1.14 2.8
-
using D-ribose as substrate, purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0) Thermotoga maritima
5.3.1.14 5.1
-
using L-fructose as substrate, purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0) Thermotoga maritima
5.3.1.14 5.3
-
using D-gulose as substrate, purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0) Thermotoga maritima
5.3.1.14 6.7
-
using D-allose as substrate, purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0) Thermotoga maritima
5.3.1.14 6.8
-
using D-ribulose as substrate, purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0) Thermotoga maritima
5.3.1.14 8.6
-
using L-xylulose as substrate, purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0) Thermotoga maritima
5.3.1.14 15
-
using L-mannose as substrate, purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0) Thermotoga maritima
5.3.1.14 20
-
using L-lyxose as substrate, purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0) Thermotoga maritima
5.3.1.14 55
-
using L-rhamnose as substrate, purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0) Thermotoga maritima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.14 D-allose
-
Thermotoga maritima D-psicose
-
r
5.3.1.14 D-gulose
-
Thermotoga maritima D-sorbose
-
r
5.3.1.14 D-ribose
-
Thermotoga maritima D-ribulose
-
r
5.3.1.14 D-ribose
-
Thermotoga maritima ATCC 43589 D-ribulose
-
r
5.3.1.14 L-fructose
-
Thermotoga maritima L-mannose
-
r
5.3.1.14 L-lyxose
-
Thermotoga maritima L-xylulose
-
r
5.3.1.14 L-lyxose
-
Thermotoga maritima ATCC 43589 L-xylulose
-
r
5.3.1.14 L-Mannose
-
Thermotoga maritima L-Fructose
-
r
5.3.1.14 L-Mannose
-
Thermotoga maritima ATCC 43589 L-Fructose
-
r
5.3.1.14 L-Rhamnose highest specific activity Thermotoga maritima L-Rhamnulose
-
r
5.3.1.14 L-Rhamnose highest specific activity Thermotoga maritima ATCC 43589 L-Rhamnulose
-
r
5.3.1.14 L-xylulose
-
Thermotoga maritima L-lyxose
-
r
5.3.1.14 additional information no activity is observed with D-ribose 5-phosphate, D-glucose 6-phosphate and D-mannose 6-phosphate Thermotoga maritima ?
-
?
5.3.1.14 additional information no activity is observed with D-ribose 5-phosphate, D-glucose 6-phosphate and D-mannose 6-phosphate Thermotoga maritima ATCC 43589 ?
-
?

Subunits

EC Number Subunits Comment Organism
5.3.1.14 tetramer 4 * 46000, SDS-PAGE Thermotoga maritima
5.3.1.14 tetramer 4 * 45527, calculated from amino acid sequence Thermotoga maritima

Synonyms

EC Number Synonyms Comment Organism
5.3.1.14 RhaA
-
Thermotoga maritima

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.3.1.14 85
-
-
Thermotoga maritima

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.3.1.14 75 95 the half-lives of the enzyme at 75°C, 80°C, 85°C, 90°C and 95°C are 773 h, 347 h, 187 h, 118 h, and 65 h, respectively Thermotoga maritima

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.3.1.14 146
-
L-rhamnose purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0) Thermotoga maritima
5.3.1.14 274
-
L-Fructose purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0) Thermotoga maritima
5.3.1.14 281
-
L-xylulose purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0) Thermotoga maritima
5.3.1.14 442
-
L-Mannose purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0) Thermotoga maritima
5.3.1.14 462
-
L-Lyxose purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0) Thermotoga maritima

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.3.1.14 8
-
-
Thermotoga maritima

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.3.1.14 2.23
-
L-Fructose purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0) Thermotoga maritima
5.3.1.14 2.55
-
L-xylulose purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0) Thermotoga maritima
5.3.1.14 5.82
-
L-Mannose purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0) Thermotoga maritima
5.3.1.14 6.72
-
L-Lyxose purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0) Thermotoga maritima
5.3.1.14 39.36
-
L-rhamnose purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0) Thermotoga maritima