Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Takami, W.; Horinouchi, M.; Nojiri, H.; Yamane, H.; Omori, T.
    Evaluation of trichloroethylene degradation by E. coli transformed with dimethyl sulfide monooxygenase genes and/or cumene dioxygenase genes (1999), Biotechnol. Lett., 21, 259-264.
No PubMed abstract available

Application

EC Number Application Comment Organism
1.14.13.245 environmental protection Acinetobacter sp. 20B grown on dimethyl sulfide degrades up to 25% of 1.5 mg trichloroethylene/l, respectively. Escherichia coli harboring the DMS monooxygenase genes from strain 20B alone, or in combination with the cumene dioxygenase genes from Pseudomonas fluorescens IP01, degrades up to 50% and 88% of 75 mg TCE/l, respectively. The growth rates of the E. coli recombinants remain nearly unaffected by TCE at least up to 150 mg/l Acinetobacter sp.

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.245 Acinetobacter sp. O32428 component dsoA
-
1.14.13.245 Acinetobacter sp. 20B O32428 component dsoA
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.245 1,1-dichloroethylene + O2 + NADH + H+ up to 33% substrate degradation Acinetobacter sp. ? + NAD+ + H2O 1,1-dichloroethylene is partially converted to corresponding dihydroxy-dichloroehtylene, and approximately 50% of the resultant dihydroxy-dichloroethylene are dechlorinated ?
1.14.13.245 1,1-dichloroethylene + O2 + NADH + H+ up to 33% substrate degradation Acinetobacter sp. 20B ? + NAD+ + H2O 1,1-dichloroethylene is partially converted to corresponding dihydroxy-dichloroehtylene, and approximately 50% of the resultant dihydroxy-dichloroethylene are dechlorinated ?
1.14.13.245 cis-1,2-dichloroethylene + O2 + NADH + H+ up to 100% substrate degradation Acinetobacter sp. cis-1,2-dichlorooxirane + NAD+ + H2O
-
 ?
1.14.13.245 cis-1,2-dichloroethylene + O2 + NADH + H+ up to 100% substrate degradation Acinetobacter sp. 20B cis-1,2-dichlorooxirane + NAD+ + H2O
-
 ?
1.14.13.245 trans-1,2-dichloroethylene + O2 + NADH + H+ up to 32% substrate degradation Acinetobacter sp. 2,3-dichlorooxirane + NAD+ + H2O
-
 ?
1.14.13.245 trans-1,2-dichloroethylene + O2 + NADH + H+ up to 32% substrate degradation Acinetobacter sp. 20B 2,3-dichlorooxirane + NAD+ + H2O
-
 ?
1.14.13.245 trichloroethylene + O2 + NADH + H+ Acinetobacter sp. 20B grown on dimethyl sulfide degrades up to 50% of 75 mg trichloroethylene/l, respectively Acinetobacter sp. ? + NAD+ + H2O
-
 ?
1.14.13.245 trichloroethylene + O2 + NADH + H+ Acinetobacter sp. 20B grown on dimethyl sulfide degrades up to 50% of 75 mg trichloroethylene/l, respectively Acinetobacter sp. 20B ? + NAD+ + H2O
-
 ?