EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.1.26.5 | additional information | generation of several enzyme mutants with deleted stem loops in their RNA part. The mutants show reduced activity | Pyrococcus horikoshii |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.26.5 | additional information | - |
additional information | kinetics of deletion mutants | Pyrococcus horikoshii | |
3.1.26.5 | 0.00000009 | - |
pre-tRNATyr | wild-type enzyme, pH not specified in the publication, 65°C | Pyrococcus horikoshii |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.26.5 | Mg2+ | required | Pyrococcus horikoshii |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.26.5 | Pyrococcus horikoshii | - |
- |
- |
3.1.26.5 | Pyrococcus horikoshii OT-3 | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.26.5 | pre-tRNATyr + H2O | activity assay using in vitro reconstituted particles | Pyrococcus horikoshii | tRNATyr + 5' leader of tRNA | - |
? | |
3.1.26.5 | pre-tRNATyr + H2O | activity assay using in vitro reconstituted particles | Pyrococcus horikoshii OT-3 | tRNATyr + 5' leader of tRNA | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.26.5 | PhoPRNA | - |
Pyrococcus horikoshii |
3.1.26.5 | RNase P RNA | - |
Pyrococcus horikoshii |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.26.5 | additional information | - |
the deletion mutants show a slightly reduced optimal temperature compared to the wild-type enzyme | Pyrococcus horikoshii |
3.1.26.5 | 65 | 70 | wild-type enzyme | Pyrococcus horikoshii |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.26.5 | 0.009 | - |
pre-tRNATyr | wild-type enzyme, pH not specified in the publication, 65°C | Pyrococcus horikoshii |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.1.26.5 | physiological function | the stem loops of the RNase P RNA are required as binding sites for the proteins, their interactions are predominantly involved in stabilizing the active conformation of the enzyme | Pyrococcus horikoshii |