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Literature summary extracted from

  • Hara, T.; Terada, A.; Yamaguchi, H.; Nakashima, T.; Kakuta, Y.; Kimura, M.
    The contribution of peripheral stem-loops to the catalytic activity of archaeal RNase P RNA from Pyrococcus horikoshii OT3 (2011), Biosci. Biotechnol. Biochem., 75, 816-819.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.1.26.5 additional information generation of several enzyme mutants with deleted stem loops in their RNA part. The mutants show reduced activity Pyrococcus horikoshii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.26.5 additional information
-
 additional information kinetics of deletion mutants Pyrococcus horikoshii
3.1.26.5 0.00000009
-
 pre-tRNATyr wild-type enzyme, pH not specified in the publication, 65°C Pyrococcus horikoshii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.26.5 Mg2+ required Pyrococcus horikoshii

Organism

EC Number Organism UniProt Comment Textmining
3.1.26.5 Pyrococcus horikoshii
-
-
-
3.1.26.5 Pyrococcus horikoshii OT-3
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.26.5 pre-tRNATyr + H2O activity assay using in vitro reconstituted particles Pyrococcus horikoshii tRNATyr + 5' leader of tRNA
-
 ?
3.1.26.5 pre-tRNATyr + H2O activity assay using in vitro reconstituted particles Pyrococcus horikoshii OT-3 tRNATyr + 5' leader of tRNA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.26.5 PhoPRNA
-
 Pyrococcus horikoshii
3.1.26.5 RNase P RNA
-
 Pyrococcus horikoshii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.26.5 additional information
-
 the deletion mutants show a slightly reduced optimal temperature compared to the wild-type enzyme Pyrococcus horikoshii
3.1.26.5 65 70 wild-type enzyme Pyrococcus horikoshii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.26.5 0.009
-
 pre-tRNATyr wild-type enzyme, pH not specified in the publication, 65°C Pyrococcus horikoshii

General Information

EC Number General Information Comment Organism
3.1.26.5 physiological function the stem loops of the RNase P RNA are required as binding sites for the proteins, their interactions are predominantly involved in stabilizing the active conformation of the enzyme Pyrococcus horikoshii