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Literature summary extracted from

  • Takata, G.; Uechi, K.; Taniguchi, E.; Kanbara, Y.; Yoshihara, A.; Morimoto, K.; Izumori, K.
    Characterization of Mesorhizobium loti L-rhamnose isomerase and its application to L-talose production (2011), Biosci. Biotechnol. Biochem., 75, 1006-1009.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.1.14 expressed in Escherichia coli JM109 cells Mesorhizobium loti

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.3.1.14 EDTA enzyme activity is lost after EDTA treatment Mesorhizobium loti

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.14 2 3.4 L-Mannose in 50 mM glycine-NaOH, pH 9.0, 1 mM MnCl2, at 60°C Mesorhizobium loti
5.3.1.14 5
-
 L-rhamnose in 50 mM glycine-NaOH, pH 9.0, 1 mM MnCl2, at 60°C Mesorhizobium loti
5.3.1.14 5.25
-
 L-talose in 50 mM glycine-NaOH, pH 9.0, 1 mM MnCl2, at 60°C Mesorhizobium loti
5.3.1.14 6.18
-
 D-ribose in 50 mM glycine-NaOH, pH 9.0, 1 mM MnCl2, at 60°C Mesorhizobium loti
5.3.1.14 7.11
-
 D-allose in 50 mM glycine-NaOH, pH 9.0, 1 mM MnCl2, at 60°C Mesorhizobium loti
5.3.1.14 23.5
-
 L-Lyxose in 50 mM glycine-NaOH, pH 9.0, 1 mM MnCl2, at 60°C Mesorhizobium loti

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.3.1.14 Mn2+ highest activity in the presence of Mn2+ Mesorhizobium loti

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.1.14 46946
-
 x * 46946, calculated from amino acid sequence Mesorhizobium loti

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.14 Mesorhizobium loti
-
-
-
5.3.1.14 Mesorhizobium loti Tono
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.1.14 Ni-NTA column chromatography Mesorhizobium loti

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.14 D-Allose
-
 Mesorhizobium loti ?
-
 ?
5.3.1.14 D-Allose
-
 Mesorhizobium loti Tono ?
-
 ?
5.3.1.14 D-allose
-
 Mesorhizobium loti D-talose
-
 ?
5.3.1.14 D-Arabinose slight activity Mesorhizobium loti ?
-
 ?
5.3.1.14 D-Galactose slight activity Mesorhizobium loti ?
-
 ?
5.3.1.14 D-glucose slight activity Mesorhizobium loti ?
-
 ?
5.3.1.14 D-Lyxose slight activity Mesorhizobium loti ?
-
 ?
5.3.1.14 D-Mannose
-
 Mesorhizobium loti ?
-
 ?
5.3.1.14 D-Mannose slight activity Mesorhizobium loti ?
-
 ?
5.3.1.14 D-ribose
-
 Mesorhizobium loti D-ribulose
-
 ?
5.3.1.14 D-ribose
-
 Mesorhizobium loti Tono D-ribulose
-
 ?
5.3.1.14 D-Ribose
-
 Mesorhizobium loti ?
-
 ?
5.3.1.14 D-talose slight activity Mesorhizobium loti D-allose
-
 ?
5.3.1.14 D-talose slight activity Mesorhizobium loti ?
-
 ?
5.3.1.14 L-Arabinose slight activity Mesorhizobium loti ?
-
 ?
5.3.1.14 L-galactose slight activity Mesorhizobium loti ?
-
 ?
5.3.1.14 L-lyxose
-
 Mesorhizobium loti L-xylulose
-
 r
5.3.1.14 L-lyxose
-
 Mesorhizobium loti Tono L-xylulose
-
 r
5.3.1.14 L-lyxose
-
 Mesorhizobium loti ?
-
 r
5.3.1.14 L-Mannose
-
 Mesorhizobium loti L-Fructose
-
 ?
5.3.1.14 L-Mannose
-
 Mesorhizobium loti Tono L-Fructose
-
 ?
5.3.1.14 L-mannose
-
 Mesorhizobium loti ?
-
 ?
5.3.1.14 L-Rhamnose highest activity Mesorhizobium loti L-Rhamnulose
-
 ?
5.3.1.14 L-Rhamnose highest activity Mesorhizobium loti Tono L-Rhamnulose
-
 ?
5.3.1.14 L-ribose slight activity Mesorhizobium loti ?
-
 ?
5.3.1.14 L-talose
-
 Mesorhizobium loti ?
-
 ?
5.3.1.14 L-xylulose
-
 Mesorhizobium loti L-lyxose
-
 r

Subunits

EC Number Subunits Comment Organism
5.3.1.14 ? x * 46946, calculated from amino acid sequence Mesorhizobium loti

Synonyms

EC Number Synonyms Comment Organism
5.3.1.14 L-RhI
-
 Mesorhizobium loti

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.3.1.14 60
-
-
 Mesorhizobium loti

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.3.1.14 50
-
 after incubation at 50°C in glycine-NaOH buffer (pH 9.0) for 1 h, approximately 70% of enzyme activity is retained Mesorhizobium loti
5.3.1.14 50
-
 after incubation at 50°C in glycine–NaOH buffer (pH 9.0) for 1 h, approximately 70% of enzyme activity is retained Mesorhizobium loti

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.3.1.14 0.1
-
 D-ribose in 50 mM glycine-NaOH, pH 9.0, 1 mM MnCl2, at 60°C Mesorhizobium loti
5.3.1.14 1.3
-
 D-allose in 50 mM glycine-NaOH, pH 9.0, 1 mM MnCl2, at 60°C Mesorhizobium loti
5.3.1.14 7
-
 L-talose in 50 mM glycine-NaOH, pH 9.0, 1 mM MnCl2, at 60°C Mesorhizobium loti
5.3.1.14 10
-
 L-Mannose in 50 mM glycine-NaOH, pH 9.0, 1 mM MnCl2, at 60°C Mesorhizobium loti
5.3.1.14 50.5
-
 L-rhamnose in 50 mM glycine-NaOH, pH 9.0, 1 mM MnCl2, at 60°C Mesorhizobium loti
5.3.1.14 73.8
-
 L-Lyxose in 50 mM glycine-NaOH, pH 9.0, 1 mM MnCl2, at 60°C Mesorhizobium loti

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.1.14 9
-
-
 Mesorhizobium loti

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
5.3.1.14 5 11 the enzyme remains stable after 24 h over a broad pH range from pH 5.0-11.0 Mesorhizobium loti

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.3.1.14 0.2
-
 D-allose in 50 mM glycine-NaOH, pH 9.0, 1 mM MnCl2, at 60°C Mesorhizobium loti
5.3.1.14 0.4
-
 L-Mannose in 50 mM glycine-NaOH, pH 9.0, 1 mM MnCl2, at 60°C Mesorhizobium loti
5.3.1.14 0.8
-
 D-ribose in 50 mM glycine-NaOH, pH 9.0, 1 mM MnCl2, at 60°C Mesorhizobium loti
5.3.1.14 1.3
-
 L-talose in 50 mM glycine-NaOH, pH 9.0, 1 mM MnCl2, at 60°C Mesorhizobium loti
5.3.1.14 3.1
-
 L-Lyxose in 50 mM glycine-NaOH, pH 9.0, 1 mM MnCl2, at 60°C Mesorhizobium loti
5.3.1.14 10.1
-
 L-rhamnose in 50 mM glycine-NaOH, pH 9.0, 1 mM MnCl2, at 60°C Mesorhizobium loti