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Literature summary extracted from

  • Wakai, S.; Tsujita, M.; Kikumoto, M.; Manchur, M.A.; Kanao, T.; Kamimura, K.
    Purification and characterization of sulfide:quinone oxidoreductase from an acidophilic iron-oxidizing bacterium, Acidithiobacillus ferrooxidans (2007), Biosci. Biotechnol. Biochem., 71, 2735-2742.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.5.4 expressed in Escherichia coli BL21(DE3) or Rosetta-gami(DE3) cells as a thioredoxin-fusion protein in inclusion bodies in an inactive form Acidithiobacillus ferrooxidans

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.5.4 2-n-heptyl-4-hydroxy-quinone-N-oxide
-
Acidithiobacillus ferrooxidans
1.8.5.4 antimycin A
-
Acidithiobacillus ferrooxidans
1.8.5.4 cyanide
-
Acidithiobacillus ferrooxidans
1.8.5.4 Myxothiazol
-
Acidithiobacillus ferrooxidans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.5.4 0.014
-
ubiquinone-2 apparent value, at pH 7.0, temperature not specified in the publication Acidithiobacillus ferrooxidans
1.8.5.4 0.042
-
Sulfide apparent value, at pH 7.0, temperature not specified in the publication Acidithiobacillus ferrooxidans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.8.5.4 membrane
-
Acidithiobacillus ferrooxidans 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.5.4 47000
-
x * 47000, SDS-PAGE Acidithiobacillus ferrooxidans
1.8.5.4 47290
-
x * 47290, deduced from amino acid sequence Acidithiobacillus ferrooxidans
1.8.5.4 60000 65000 native enzyme, gel filtration Acidithiobacillus ferrooxidans

Organism

EC Number Organism UniProt Comment Textmining
1.8.5.4 Acidithiobacillus ferrooxidans
-
-
-
1.8.5.4 Acidithiobacillus ferrooxidans NASF-1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.5.4 ammonium sulfate precipitation, phenyl-Toyopearl column chromatography, and TSKgel G3000 gel filtration Acidithiobacillus ferrooxidans

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.8.5.4 culture condition:sulfur-grown cell
-
Acidithiobacillus ferrooxidans
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.5.4 0.11
-
cell-free extract, at pH 7.0, temperature not specified in the publication Acidithiobacillus ferrooxidans
1.8.5.4 20.55
-
after 186.8fold purification, at pH 7.0, temperature not specified in the publication Acidithiobacillus ferrooxidans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.5.4 sulfide + ubiquinone-2
-
Acidithiobacillus ferrooxidans sulfur + ubiquinol-2
-
?
1.8.5.4 sulfide + ubiquinone-2
-
Acidithiobacillus ferrooxidans NASF-1 sulfur + ubiquinol-2
-
?

Subunits

EC Number Subunits Comment Organism
1.8.5.4 ? x * 47000, SDS-PAGE Acidithiobacillus ferrooxidans
1.8.5.4 ? x * 47290, deduced from amino acid sequence Acidithiobacillus ferrooxidans

Synonyms

EC Number Synonyms Comment Organism
1.8.5.4 SQR
-
Acidithiobacillus ferrooxidans
1.8.5.4 sulfide:quinone oxidoreductase
-
Acidithiobacillus ferrooxidans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.5.4 7
-
-
Acidithiobacillus ferrooxidans

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.5.4 FAD
-
Acidithiobacillus ferrooxidans

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.8.5.4 Acidithiobacillus ferrooxidans deduced from amino acid sequence
-
6.5

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.8.5.4 0.039
-
at pH 7.0, temperature not specified in the publication Acidithiobacillus ferrooxidans Myxothiazol
1.8.5.4 0.05
-
at pH 7.0, temperature not specified in the publication Acidithiobacillus ferrooxidans 2-n-heptyl-4-hydroxy-quinone-N-oxide
1.8.5.4 0.22
-
at pH 7.0, temperature not specified in the publication Acidithiobacillus ferrooxidans antimycin A
1.8.5.4 0.54
-
at pH 7.0, temperature not specified in the publication Acidithiobacillus ferrooxidans cyanide

General Information

EC Number General Information Comment Organism
1.8.5.4 metabolism SQR is involved in elemental sulfur oxidation in sulfur-grown cells Acidithiobacillus ferrooxidans