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Literature summary extracted from

  • Kim, d.o..Y.; Ham, S.J.; Lee, H.J.; Cho, H.Y.; Kim, J.H.; Kim, Y.J.; Shin, D.H.; Rhee, Y.H.; Son, K.H.; Park, H.Y.
    Cloning and characterization of a modular GH5 beta-1,4-mannanase with high specific activity from the fibrolytic bacterium Cellulosimicrobium sp. strain HY-13 (2011), Biores. Technol., 102, 9185-9192.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.78 Sodium azide hydrolysis of locust bean gum is enhanced by approximately 1.45fold in poresence of sodium azide Cellulosimicrobium sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.78 expression in Escherichia coli Cellulosimicrobium sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.78 Cu2+ more than 50% inhibition Cellulosimicrobium sp.
3.2.1.78 EDTA 5 mM, 20% residaul activity Cellulosimicrobium sp.
3.2.1.78 Fe2+ more than 50% inhibition Cellulosimicrobium sp.
3.2.1.78 Zn2+ more than 50% inhibition Cellulosimicrobium sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.78 43767
-
 x * 44000, SDS-PAGE, x * 43767, calculated Cellulosimicrobium sp.
3.2.1.78 44000
-
 x * 44000, SDS-PAGE, x * 43767, calculated Cellulosimicrobium sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.78 Cellulosimicrobium sp. G3FBL1 member of glycosyl hydrolase family 5
-
3.2.1.78 Cellulosimicrobium sp. HY-13 G3FBL1 member of glycosyl hydrolase family 5
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.78 proteolytic modification sequence contains a N-terminal signal peptide of 24 amino acids Cellulosimicrobium sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.78 8498
-
 substrate locust bean gum, pH 6.0, 50°C Cellulosimicrobium sp.
3.2.1.78 14710
-
 substrate ivory nut mannan, pH 6.0, 50°C Cellulosimicrobium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.78 ivory nut mannan + H2O
-
 Cellulosimicrobium sp. mannotriose + mannotetraose + mannopentaose + mannohexaose main products, at 14%, 25%, 20%, and 13%, respectively ?
3.2.1.78 ivory nut mannan + H2O
-
 Cellulosimicrobium sp. HY-13 mannotriose + mannotetraose + mannopentaose + mannohexaose main products, at 14%, 25%, 20%, and 13%, respectively ?
3.2.1.78 locust bean gum + H2O
-
 Cellulosimicrobium sp. mannopentaose 44%, main product ?
3.2.1.78 locust bean gum + H2O
-
 Cellulosimicrobium sp. HY-13 mannopentaose 44%, main product ?
3.2.1.78 additional information no substrate: mannobiose, or 4-nitrophenyl sugar derivatives Cellulosimicrobium sp. ?
-
 ?
3.2.1.78 additional information no substrate: mannobiose, or 4-nitrophenyl sugar derivatives Cellulosimicrobium sp. HY-13 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.78 ? x * 44000, SDS-PAGE, x * 43767, calculated Cellulosimicrobium sp.

Synonyms

EC Number Synonyms Comment Organism
3.2.1.78 ManH
-
 Cellulosimicrobium sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.78 50
-
-
 Cellulosimicrobium sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.78 50
-
 half-life 15 min Cellulosimicrobium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.78 6
-
-
 Cellulosimicrobium sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.78 5.5 9 1 h, more than 80% residual activity Cellulosimicrobium sp.

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.78 Cellulosimicrobium sp. calculated
-
 4.6

General Information

EC Number General Information Comment Organism
3.2.1.78 physiological function enzyme strongly binds to ivory nut mannan, Avicel, chitosan, and chitin, but does not attach to curdlan, insoluble oat spelt xylan, lignin, or poly(3-hydroxybutyrate) Cellulosimicrobium sp.