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Literature summary extracted from

  • Lim, Y.R.; Yeom, S.J.; Kim, Y.S.; Oh, D.K.
    Synergistic production of L-arabinose from arabinan by the combined use of thermostable endo- and exo-arabinanases from Caldicellulosiruptor saccharolyticus (2011), Biores. Technol., 102, 4277-4280.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.99 expressed in Escherichia coli ER2566 cells Caldicellulosiruptor saccharolyticus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.99 Caldicellulosiruptor saccharolyticus
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-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.99 His-Trap column chromatography, gel filtration Caldicellulosiruptor saccharolyticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.99 debranched (1->5)-L-arabinan + H2O under the optimum conditions, 16 g/l L-arabinose is obtained from 20 g/l debranched arabinan with a hydrolysis yield of 80% Caldicellulosiruptor saccharolyticus L-arabinose
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 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.99 endo-1,5-alpha-L-arabinanase
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 Caldicellulosiruptor saccharolyticus
3.2.1.99 endo-arabinase
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 Caldicellulosiruptor saccharolyticus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.99 75
-
-
 Caldicellulosiruptor saccharolyticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.99 6
-
 using sugar beet arabinan as substrate Caldicellulosiruptor saccharolyticus
3.2.1.99 6.5
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 using debranched arabinan as substrate Caldicellulosiruptor saccharolyticus