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Literature summary extracted from

  • Dheeman, D.S.; Henehan, G.T.; Frias, J.M.
    Purification and properties of Amycolatopsis mediterranei DSM 43304 lipase and its potential in flavour ester synthesis (2011), Biores. Technol., 102, 3373-3379.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.3 ascorbic acid 124.4% activity at 1 mM Amycolatopsis mediterranei
3.1.1.3 beta-mercaptoethanol 136.4% activity at 1 mM Amycolatopsis mediterranei
3.1.1.3 Digitonin 142.5% activity at 1 mM Amycolatopsis mediterranei
3.1.1.3 dithiothreitol 147.6% activity at 1 mM Amycolatopsis mediterranei
3.1.1.3 sodium deoxycholate 241.3% activity at 1 mM Amycolatopsis mediterranei

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.3 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide complete inhibition at 5 mM Amycolatopsis mediterranei
3.1.1.3 capric acid 89.7% residual activity at 1 mM Amycolatopsis mediterranei
3.1.1.3 diethyldicarbonate complete inhibition at 5 mM Amycolatopsis mediterranei
3.1.1.3 Hg2+ Hg2+ shows the highest reduction in enzyme activity by 83.3% at 1 mM Amycolatopsis mediterranei
3.1.1.3 additional information not affected by EDTA, sodium citrate, SDS, myristic acid, palmitic acid, and urea (6.0 M). The enzyme is not inhibited by iodoacetate, citraconic anhydride, phenylglyoxal, and N-acetylimidazole Amycolatopsis mediterranei
3.1.1.3 N-bromosuccinimide 34.4% residual activity at 5 mM Amycolatopsis mediterranei
3.1.1.3 phenylmethylsulfonyl fluoride 11.6% residual activity at 5 mM Amycolatopsis mediterranei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.3 0.099
-
 4-nitrophenyl palmitate pH 8.0, 60°C Amycolatopsis mediterranei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.3 33000
-
 x * 33000, SDS-PAGE Amycolatopsis mediterranei

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Amycolatopsis mediterranei
-
-
-
3.1.1.3 Amycolatopsis mediterranei DSM 43304
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.3 ammonium sulfate precipitation, Q Sepharose column chromatography, and Toyopearl phenyl-650M column chromatography (398fold purification with 36% final recovery) Amycolatopsis mediterranei

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.3 1.96
-
 enzyme from culture filtrate, using 4-nitrophenyl palmitate as substrate, at pH 8.0 and 60°C Amycolatopsis mediterranei
3.1.1.3 781.6
-
 enzyme after 398.79fold purification, using 4-nitrophenyl palmitate as substrate, at pH 8.0 and 60°C Amycolatopsis mediterranei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 4 triolein + H2O about 115% activity compared to olive oil Amycolatopsis mediterranei 6 oleic acid + 1,3-diolein + 1,2-diolein + 1-monoolein + 2-monoolein oleic acid is the major product ?
3.1.1.3 4-nitrophenyl acetate + H2O about 88% activity compared to 4-nitrophenyl palmitate Amycolatopsis mediterranei 4-nitrophenol + acetate
-
 ?
3.1.1.3 4-nitrophenyl acetate + H2O about 88% activity compared to 4-nitrophenyl palmitate Amycolatopsis mediterranei DSM 43304 4-nitrophenol + acetate
-
 ?
3.1.1.3 4-nitrophenyl butyrate + H2O about 90% activity compared to 4-nitrophenyl palmitate Amycolatopsis mediterranei 4-nitrophenol + butyrate
-
 ?
3.1.1.3 4-nitrophenyl butyrate + H2O about 90% activity compared to 4-nitrophenyl palmitate Amycolatopsis mediterranei DSM 43304 4-nitrophenol + butyrate
-
 ?
3.1.1.3 4-nitrophenyl caproate + H2O second-highest hydrolysis rate, about 95% activity compared to 4-nitrophenyl palmitate Amycolatopsis mediterranei 4-nitrophenol + caproate
-
 ?
3.1.1.3 4-nitrophenyl caproate + H2O second-highest hydrolysis rate, about 95% activity compared to 4-nitrophenyl palmitate Amycolatopsis mediterranei DSM 43304 4-nitrophenol + caproate
-
 ?
3.1.1.3 4-nitrophenyl caprylate + H2O highest hydrolysis rate, about 90% activity compared to 4-nitrophenyl palmitate Amycolatopsis mediterranei 4-nitrophenol + caprylate
-
 ?
3.1.1.3 4-nitrophenyl laurate + H2O about 60% activity compared to 4-nitrophenyl palmitate Amycolatopsis mediterranei 4-nitrophenol + laurate
-
 ?
3.1.1.3 4-nitrophenyl myristate + H2O about 30% activity compared to 4-nitrophenyl palmitate Amycolatopsis mediterranei 4-nitrophenol + myristate
-
 ?
3.1.1.3 4-nitrophenyl palmitate + H2O the purified enzyme is most active toward 4-nitrophenyl palmitate (100% activity) Amycolatopsis mediterranei 4-nitrophenol + palmitate
-
 ?
3.1.1.3 isoamyl alcohol + acetic acid after 72 h of reaction a conversion yield of 34.4% and 16.2% of isoamyl acetate is obtained using cellite-immobilized and free enzyme, respectively Amycolatopsis mediterranei isoamyl acetate
-
 ?
3.1.1.3 additional information the enzyme also uses olive oil (100% activity), corn oil (about 85% activity), castor oil (about 80% activity), sunflower oil (about 95% activity), rape seed oil (about 85% activity), linseed oil (about 75% activity), cotton seed (about 90% activity), and jojoba oil (about 83% activity) as substrates Amycolatopsis mediterranei ?
-
 ?
3.1.1.3 additional information the enzyme also uses olive oil (100% activity), corn oil (about 85% activity), castor oil (about 80% activity), sunflower oil (about 95% activity), rape seed oil (about 85% activity), linseed oil (about 75% activity), cotton seed (about 90% activity), and jojoba oil (about 83% activity) as substrates Amycolatopsis mediterranei DSM 43304 ?
-
 ?
3.1.1.3 trilinolein + H2O about 105% activity compared to olive oil Amycolatopsis mediterranei ?
-
 ?
3.1.1.3 trilinolenin + H2O about 100% activity compared to olive oil Amycolatopsis mediterranei ?
-
 ?
3.1.1.3 trioctanoin + H2O about 59% activity compared to olive oil Amycolatopsis mediterranei ?
-
 ?
3.1.1.3 trioctanoin + H2O about 59% activity compared to olive oil Amycolatopsis mediterranei DSM 43304 ?
-
 ?
3.1.1.3 tripalmitin + H2O about 45% activity compared to olive oil Amycolatopsis mediterranei ?
-
 ?
3.1.1.3 tristearin + H2O about 38% activity compared to olive oil Amycolatopsis mediterranei ?
-
 ?
3.1.1.3 trivaccinin + H2O about 78% activity compared to olive oil Amycolatopsis mediterranei ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.3 ? x * 33000, SDS-PAGE Amycolatopsis mediterranei

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 lipase
-
 Amycolatopsis mediterranei

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.3 60
-
-
 Amycolatopsis mediterranei

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.3 50 60 the purified enzyme is most stable at 50-60°C retaining 100% activity after 3 h Amycolatopsis mediterranei

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.3 1468
-
 4-nitrophenyl palmitate apparent value, in 50 mM Tris-HCl buffer, at pH 8.0, 60°C Amycolatopsis mediterranei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.3 8
-
-
 Amycolatopsis mediterranei

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.3 7 9 the activity is not much affected at pH 7.0 and 9.0 where it shows around 90% of relative activity Amycolatopsis mediterranei

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.3 6 9 the purified enzyme is stable in the pH range 6.0-9.0 retaining more than 95% of relative activity after 24 h of incubation Amycolatopsis mediterranei