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Literature summary extracted from
- A new beta-carbonic anhydrase from Brucella suis, its cloning, characterization, and inhibition with sulfonamides and sulfamates, leading to impaired pathogen growth (2011), Bioorg. Med. Chem., 19, 1172-1178.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.2.1.1 | beta class isozymes bsCA I and bsCA II, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of His-tagged bsCA II in Escherichia coli | Brucella suis |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.1 | 2-aminobenzenesulfonamide | - |
Brucella suis |  |
| 4.2.1.1 | 2-hydrazinylbenzenesulfonamide | - |
Brucella suis | |
| 4.2.1.1 | 4-(2-aminoethyl)benzenesulfonamide | - |
Brucella suis | |
| 4.2.1.1 | 4-(2-hydroxyethyl)benzenesulfonamide | - |
Brucella suis | |
| 4.2.1.1 | 4-(aminomethyl)benzenesulfonamide | - |
Brucella suis | |
| 4.2.1.1 | 4-(hydroxymethyl)benzenesulfonamide | - |
Brucella suis | |
| 4.2.1.1 | 4-amino-3,5-dibromobenzenesulfonamide | - |
Brucella suis | |
| 4.2.1.1 | 4-amino-3,5-diiodobenzenesulfonamide | - |
Brucella suis | |
| 4.2.1.1 | 4-amino-3-chlorobenzenesulfonamide | - |
Brucella suis | |
| 4.2.1.1 | 4-amino-3-fluorobenzenesulfonamide | - |
Brucella suis | |
| 4.2.1.1 | 4-amino-6-(trifluoromethyl)benzene-1,3-disulfonamide | - |
Brucella suis | |
| 4.2.1.1 | 4-amino-6-chlorobenzene-1,3-disulfonamide | - |
Brucella suis | |
| 4.2.1.1 | 4-amino-N-(4-sulfamoylbenzyl)benzenesulfonamide | - |
Brucella suis | |
| 4.2.1.1 | 4-amino-N-[2-(4-sulfamoylphenyl)ethyl]benzenesulfonamide | - |
Brucella suis | |
| 4.2.1.1 | 4-Aminobenzenesulfonamide | - |
Brucella suis | |
| 4.2.1.1 | 4-chlorophenyl sulfamate | - |
Brucella suis | |
| 4.2.1.1 | 4-hydrazinylbenzenesulfonamide | - |
Brucella suis | |
| 4.2.1.1 | 4-methylbenzenesulfonamide | - |
Brucella suis | |
| 4.2.1.1 | 4-sulfamoyl-N-[2-(4-sulfamoylphenyl)ethyl]benzamide | - |
Brucella suis | |
| 4.2.1.1 | 4-sulfamoylbenzoic acid | - |
Brucella suis | |
| 4.2.1.1 | 4-[(2-aminopyrimidin-4-yl)amino]benzenesulfonamide | - |
Brucella suis | |
| 4.2.1.1 | 5-(2-chlorophenyl)-1,3,4-thiadiazole-2-sulfonamide | - |
Brucella suis | |
| 4.2.1.1 | 5-amino-1,3,4-thiadiazole-2-sulfonamide | - |
Brucella suis | |
| 4.2.1.1 | 5-imino-4-methyl-4,5-dihydro-1,3,4-thiadiazole-2-sulfonamide | - |
Brucella suis | |
| 4.2.1.1 | 5-[[(4-amino-3-chloro-5-fluorophenyl)sulfonyl]amino]-4,5-dihydro-1,3,4-thiadiazole-2-sulfonamide | - |
Brucella suis | |
| 4.2.1.1 | 5-[[(4-aminophenyl)sulfonyl]amino]-1,3,4-thiadiazole-2-sulfonamide | - |
Brucella suis | |
| 4.2.1.1 | 6-deoxy-N-(4-sulfamoylphenyl)-alpha-L-mannopyranosylamine | - |
Brucella suis | |
| 4.2.1.1 | 6-hydroxy-1,3-benzothiazole-2-sulfonamide | - |
Brucella suis | |
| 4.2.1.1 | acetazolamide | - |
Brucella suis | |
| 4.2.1.1 | heptane-1,7-diyl disulfamate | - |
Brucella suis | |
| 4.2.1.1 | additional information | best bsCA II inhibitors are some glycosylated sulfanilamides, aliphatic sulfamates, and halogenated sulfanilamides | Brucella suis | |
| 4.2.1.1 | N-(4-sulfamoylphenyl)-beta-D-galactopyranosylamine | - |
Brucella suis | |
| 4.2.1.1 | N-(4-sulfamoylphenyl)-beta-D-idopyranosylamine | - |
Brucella suis | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.1 | Zn2+ | beta class CAs contain four protein zinc ligands, one His, two Cys, and one Asp coordinated to Zn(II). At pH 8.0, no water is coordinated to the metal ion | Brucella suis | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.1 | 25000 | - |
x * 25000, recombinant bsCA II, SDS-PAGE | Brucella suis |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.1 | H2CO3 | Brucella suis | - |
CO2 + H2O | - |
r | |
| 4.2.1.1 | H2CO3 | Brucella suis 1330 | - |
CO2 + H2O | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.1.1 | Brucella suis | A0A0H3GB68 | two beta class isozymes bsCA I and bsCA II, gene BR1829 | - |
| 4.2.1.1 | Brucella suis 1330 | A0A0H3GB68 | two beta class isozymes bsCA I and bsCA II, gene BR1829 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.2.1.1 | recombinant His-tagged bsCA II from Escherichia coli by cobalt affinityy chromatography | Brucella suis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.1 | H2CO3 | - |
Brucella suis | CO2 + H2O | - |
r | |
| 4.2.1.1 | H2CO3 | - |
Brucella suis 1330 | CO2 + H2O | - |
r | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.2.1.1 | ? | x * 25000, recombinant bsCA II, SDS-PAGE | Brucella suis |
| 4.2.1.1 | More | bsCA II shows pH-dependent closed or open conformation, overview | Brucella suis |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.2.1.1 | beta-carbonic anhydrase | - |
Brucella suis |
| 4.2.1.1 | bsCA I | - |
Brucella suis |
| 4.2.1.1 | bsCA II | - |
Brucella suis |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.1.1 | 11000 | - |
CO2 | isozyme bsCAII, pH 8.3, temperature not specified in the publication | Brucella suis | |
| 4.2.1.1 | 64000 | - |
CO2 | isozyme bsCAI, pH 8.3, temperature not specified in the publication | Brucella suis | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.1 | 8.3 | - |
assay at | Brucella suis |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.1.1 | 0.0000073 | - |
6-deoxy-N-(4-sulfamoylphenyl)-alpha-L-mannopyranosylamine | pH 8.3, temperature not specified in the publication, isozyme bsCA II | Brucella suis | |
| 4.2.1.1 | 0.0000089 | - |
N-(4-sulfamoylphenyl)-beta-D-idopyranosylamine | pH 8.3, temperature not specified in the publication, isozyme bsCA I | Brucella suis | |
| 4.2.1.1 | 0.0000092 | - |
N-(4-sulfamoylphenyl)-beta-D-galactopyranosylamine | pH 8.3, temperature not specified in the publication, isozyme bsCA I | Brucella suis | |
| 4.2.1.1 | 0.0000101 | - |
N-(4-sulfamoylphenyl)-beta-D-galactopyranosylamine | pH 8.3, temperature not specified in the publication, isozyme bsCA II | Brucella suis | |
| 4.2.1.1 | 0.0000112 | - |
5-imino-4-methyl-4,5-dihydro-1,3,4-thiadiazole-2-sulfonamide | pH 8.3, temperature not specified in the publication, isozyme bsCA II | Brucella suis | |
| 4.2.1.1 | 0.0000146 | - |
N-(4-sulfamoylphenyl)-beta-D-idopyranosylamine | pH 8.3, temperature not specified in the publication, isozyme bsCA II | Brucella suis | |
| 4.2.1.1 | 0.000019 | - |
6-deoxy-N-(4-sulfamoylphenyl)-alpha-L-mannopyranosylamine | pH 8.3, temperature not specified in the publication, isozyme bsCA I | Brucella suis | |
| 4.2.1.1 | 0.000021 | - |
4-[(2-aminopyrimidin-4-yl)amino]benzenesulfonamide | pH 8.3, temperature not specified in the publication, isozyme bsCA I | Brucella suis | |
| 4.2.1.1 | 0.000027 | - |
4-sulfamoyl-N-[2-(4-sulfamoylphenyl)ethyl]benzamide | pH 8.3, temperature not specified in the publication, isozyme bsCA I | Brucella suis | |
| 4.2.1.1 | 0.000033 | - |
5-(2-chlorophenyl)-1,3,4-thiadiazole-2-sulfonamide | pH 8.3, temperature not specified in the publication, isozyme bsCA I | Brucella suis | |
| 4.2.1.1 | 0.000048 | - |
6-hydroxy-1,3-benzothiazole-2-sulfonamide | pH 8.3, temperature not specified in the publication, isozyme bsCA I | Brucella suis | |
| 4.2.1.1 | 0.00006 | - |
4-amino-3,5-dibromobenzenesulfonamide | pH 8.3, temperature not specified in the publication, isozyme bsCA II | Brucella suis | |
| 4.2.1.1 | 0.000063 | - |
acetazolamide | isozyme bsCAI, pH 8.3, temperature not specified in the publication | Brucella suis | |
| 4.2.1.1 | 0.000068 | - |
4-amino-N-[2-(4-sulfamoylphenyl)ethyl]benzenesulfonamide | pH 8.3, temperature not specified in the publication, isozyme bsCA II | Brucella suis | |
| 4.2.1.1 | 0.00007 | - |
5-[[(4-aminophenyl)sulfonyl]amino]-1,3,4-thiadiazole-2-sulfonamide | pH 8.3, temperature not specified in the publication, isozyme bsCA I | Brucella suis | |
| 4.2.1.1 | 0.000075 | - |
heptane-1,7-diyl disulfamate | pH 8.3, temperature not specified in the publication, isozyme bsCA I | Brucella suis | |
| 4.2.1.1 | 0.000079 | - |
4-chlorophenyl sulfamate | pH 8.3, temperature not specified in the publication, isozyme bsCA I | Brucella suis | |
| 4.2.1.1 | 0.000079 | - |
4-amino-3-chlorobenzenesulfonamide | pH 8.3, temperature not specified in the publication, isozyme bsCA II | Brucella suis | |
| 4.2.1.1 | 0.000083 | - |
4-amino-3-fluorobenzenesulfonamide | pH 8.3, temperature not specified in the publication, isozyme bsCA II | Brucella suis | |
| 4.2.1.1 | 0.000083 | - |
6-hydroxy-1,3-benzothiazole-2-sulfonamide | pH 8.3, temperature not specified in the publication, isozyme bsCA II | Brucella suis | |
| 4.2.1.1 | 0.000084 | - |
4-amino-3,5-diiodobenzenesulfonamide | pH 8.3, temperature not specified in the publication, isozyme bsCA II | Brucella suis | |
| 4.2.1.1 | 0.000087 | - |
4-amino-6-(trifluoromethyl)benzene-1,3-disulfonamide | pH 8.3, temperature not specified in the publication, isozyme bsCA II | Brucella suis | |
| 4.2.1.1 | 0.00009 | - |
4-sulfamoyl-N-[2-(4-sulfamoylphenyl)ethyl]benzamide | pH 8.3, temperature not specified in the publication, isozyme bsCA II | Brucella suis | |
| 4.2.1.1 | 0.000094 | - |
4-amino-N-(4-sulfamoylbenzyl)benzenesulfonamide | pH 8.3, temperature not specified in the publication, isozyme bsCA II | Brucella suis | |
| 4.2.1.1 | 0.000108 | - |
5-[[(4-aminophenyl)sulfonyl]amino]-1,3,4-thiadiazole-2-sulfonamide | pH 8.3, temperature not specified in the publication, isozyme bsCA II | Brucella suis | |
| 4.2.1.1 | 0.000166 | - |
5-(2-chlorophenyl)-1,3,4-thiadiazole-2-sulfonamide | pH 8.3, temperature not specified in the publication, isozyme bsCA II | Brucella suis | |
| 4.2.1.1 | 0.000186 | - |
5-[[(4-amino-3-chloro-5-fluorophenyl)sulfonyl]amino]-4,5-dihydro-1,3,4-thiadiazole-2-sulfonamide | pH 8.3, temperature not specified in the publication, isozyme bsCA I | Brucella suis | |
| 4.2.1.1 | 0.000193 | - |
heptane-1,7-diyl disulfamate | pH 8.3, temperature not specified in the publication, isozyme bsCA II | Brucella suis | |
| 4.2.1.1 | 0.000215 | - |
4-chlorophenyl sulfamate | pH 8.3, temperature not specified in the publication, isozyme bsCA II | Brucella suis | |
| 4.2.1.1 | 0.000243 | - |
4-amino-3,5-dibromobenzenesulfonamide | pH 8.3, temperature not specified in the publication, isozyme bsCA I | Brucella suis | |
| 4.2.1.1 | 0.000303 | - |
acetazolamide | isozyme bsCAII, pH 8.3, temperature not specified in the publication | Brucella suis | |
| 4.2.1.1 | 0.00034 | - |
4-sulfamoylbenzoic acid | pH 8.3, temperature not specified in the publication, isozyme bsCA I | Brucella suis | |
| 4.2.1.1 | 0.000345 | - |
4-amino-3,5-diiodobenzenesulfonamide | pH 8.3, temperature not specified in the publication, isozyme bsCA I | Brucella suis | |
| 4.2.1.1 | 0.000663 | - |
5-[[(4-amino-3-chloro-5-fluorophenyl)sulfonyl]amino]-4,5-dihydro-1,3,4-thiadiazole-2-sulfonamide | pH 8.3, temperature not specified in the publication, isozyme bsCA II | Brucella suis | |
| 4.2.1.1 | 0.000691 | - |
4-amino-6-chlorobenzene-1,3-disulfonamide | pH 8.3, temperature not specified in the publication, isozyme bsCA II | Brucella suis | |
| 4.2.1.1 | 0.000697 | - |
4-sulfamoylbenzoic acid | pH 8.3, temperature not specified in the publication, isozyme bsCA II | Brucella suis | |
| 4.2.1.1 | 0.000745 | - |
4-amino-N-[2-(4-sulfamoylphenyl)ethyl]benzenesulfonamide | pH 8.3, temperature not specified in the publication, isozyme bsCA I | Brucella suis | |
| 4.2.1.1 | 0.000754 | - |
4-(hydroxymethyl)benzenesulfonamide | pH 8.3, temperature not specified in the publication, isozyme bsCA I | Brucella suis | |
| 4.2.1.1 | 0.000758 | - |
4-(hydroxymethyl)benzenesulfonamide | pH 8.3, temperature not specified in the publication, isozyme bsCA II | Brucella suis | |
| 4.2.1.1 | 0.000768 | - |
4-(aminomethyl)benzenesulfonamide | pH 8.3, temperature not specified in the publication, isozyme bsCA I | Brucella suis | |
| 4.2.1.1 | 0.000769 | - |
4-[(2-aminopyrimidin-4-yl)amino]benzenesulfonamide | pH 8.3, temperature not specified in the publication, isozyme bsCA II | Brucella suis | |
| 4.2.1.1 | 0.0008 | - |
4-amino-3-chlorobenzenesulfonamide | pH 8.3, temperature not specified in the publication, isozyme bsCA I | Brucella suis | |
| 4.2.1.1 | 0.000847 | - |
5-amino-1,3,4-thiadiazole-2-sulfonamide | pH 8.3, temperature not specified in the publication, isozyme bsCA II | Brucella suis | |
| 4.2.1.1 | 0.000865 | - |
4-(2-hydroxyethyl)benzenesulfonamide | pH 8.3, temperature not specified in the publication, isozyme bsCA I | Brucella suis | |
| 4.2.1.1 | 0.00088 | - |
4-(2-aminoethyl)benzenesulfonamide | pH 8.3, temperature not specified in the publication, isozyme bsCA I | Brucella suis | |
| 4.2.1.1 | 0.00094 | - |
4-amino-6-chlorobenzene-1,3-disulfonamide | pH 8.3, temperature not specified in the publication, isozyme bsCA I | Brucella suis | |
| 4.2.1.1 | 0.001035 | - |
2-hydrazinylbenzenesulfonamide | pH 8.3, temperature not specified in the publication, isozyme bsCA I | Brucella suis | |
| 4.2.1.1 | 0.00105 | - |
4-amino-N-(4-sulfamoylbenzyl)benzenesulfonamide | pH 8.3, temperature not specified in the publication, isozyme bsCA I | Brucella suis | |
| 4.2.1.1 | 0.00107 | - |
4-amino-3-fluorobenzenesulfonamide | pH 8.3, temperature not specified in the publication, isozyme bsCA I | Brucella suis | |
| 4.2.1.1 | 0.00121 | - |
5-amino-1,3,4-thiadiazole-2-sulfonamide | pH 8.3, temperature not specified in the publication, isozyme bsCA I | Brucella suis | |
| 4.2.1.1 | 0.00143 | - |
5-imino-4-methyl-4,5-dihydro-1,3,4-thiadiazole-2-sulfonamide | pH 8.3, temperature not specified in the publication, isozyme bsCA I | Brucella suis | |
| 4.2.1.1 | 0.00158 | - |
4-methylbenzenesulfonamide | pH 8.3, temperature not specified in the publication, isozyme bsCA I | Brucella suis | |
| 4.2.1.1 | 0.0024 | - |
4-hydrazinylbenzenesulfonamide | pH 8.3, temperature not specified in the publication, isozyme bsCA I | Brucella suis | |
| 4.2.1.1 | 0.0025 | - |
4-Aminobenzenesulfonamide | pH 8.3, temperature not specified in the publication, isozyme bsCA I | Brucella suis | |
| 4.2.1.1 | 0.004763 | - |
4-hydrazinylbenzenesulfonamide | pH 8.3, temperature not specified in the publication, isozyme bsCA II | Brucella suis | |
| 4.2.1.1 | 0.00483 | - |
4-amino-6-(trifluoromethyl)benzene-1,3-disulfonamide | pH 8.3, temperature not specified in the publication, isozyme bsCA I | Brucella suis | |
| 4.2.1.1 | 0.005048 | - |
2-aminobenzenesulfonamide | pH 8.3, temperature not specified in the publication, isozyme bsCA II | Brucella suis | |
| 4.2.1.1 | 0.00508 | - |
4-Aminobenzenesulfonamide | pH 8.3, temperature not specified in the publication, isozyme bsCA II | Brucella suis | |
| 4.2.1.1 | 0.00513 | - |
4-(2-hydroxyethyl)benzenesulfonamide | pH 8.3, temperature not specified in the publication, isozyme bsCA II | Brucella suis | |
| 4.2.1.1 | 0.00556 | - |
4-methylbenzenesulfonamide | pH 8.3, temperature not specified in the publication, isozyme bsCA II | Brucella suis | |
| 4.2.1.1 | 0.005639 | - |
2-hydrazinylbenzenesulfonamide | pH 8.3, temperature not specified in the publication, isozyme bsCA II | Brucella suis | |
| 4.2.1.1 | 0.00587 | - |
2-aminobenzenesulfonamide | pH 8.3, temperature not specified in the publication, isozyme bsCA I | Brucella suis | |
| 4.2.1.1 | 0.00591 | - |
4-(aminomethyl)benzenesulfonamide | pH 8.3, temperature not specified in the publication, isozyme bsCA II | Brucella suis | |
| 4.2.1.1 | 0.00856 | - |
4-(2-aminoethyl)benzenesulfonamide | pH 8.3, temperature not specified in the publication, isozyme bsCA II | Brucella suis | |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 4.2.1.1 | evolution | evolutionary tree of some bacterial beta-CAs among which the two Brucella suis isozymes bsCA I and bsCA II, phylogenetic analysis, overview | Brucella suis |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.1.1 | 3900 | - |
CO2 | isozyme bsCAI, pH 8.3, temperature not specified in the publication | Brucella suis | |
| 4.2.1.1 | 8900 | - |
CO2 | isozyme bsCAII, pH 8.3, temperature not specified in the publication | Brucella suis | |
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