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Literature summary extracted from

  • Yu, C.; Xia, B.; Jin, C.
    (1)H, (13)C and (15)N resonance assignments of the arsenate reductase from Synechocystis sp. strain PCC 6803 (2010), Biomol. NMR Assign., 5, 85-87.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.20.4.1 expressed in Escherichia coli as a His-tagged fusion protein Synechocystis sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.20.4.1 14400
-
-
 Synechocystis sp.
1.20.4.1 14400
-
 x * 14400, SDS-PAGE Synechocystis sp.

Organism

EC Number Organism UniProt Comment Textmining
1.20.4.1 Synechocystis sp.
-
 ArsC protein from cyanobacterium Synechocystis sp. strain PCC 6803 (SynArsC) is related to the thioredoxin-dependent ArsC family, but uses the glutathione/glutaredoxin system for arsenate reduction (thioredoxin/glutaredoxin hybrid arsenate reductase)
-
1.20.4.1 Synechocystis sp. P74313
-
-
1.20.4.4 Microbacterium sp. B7FAZ6
-
-
1.20.4.4 Microbacterium sp. B7FB01
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.20.4.1 using Ni-NTA chromatography, gel filtration Synechocystis sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.20.4.1 additional information chemical shift assignments of 1H, 13C and 15N atoms for the reduced form the enzyme Synechocystis sp. ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.20.4.1 ? x * 14400, SDS-PAGE Synechocystis sp.

Synonyms

EC Number Synonyms Comment Organism
1.20.4.1 arsenate reductase
-
 Synechocystis sp.
1.20.4.1 slr0946
-
 Synechocystis sp.
1.20.4.1 SynArsC
-
 Synechocystis sp.
1.20.4.4 ArsC1 ArsC1 is related to thioredoxin-dependent arsenate reductases Microbacterium sp.
1.20.4.4 ArsC3 ArsC3 is related to thioredoxin-dependent arsenate reductases but ArsC3 lacks the two distal catalytic cysteine residues of this class of enzymes Microbacterium sp.
1.20.4.4 arsenate reductase
-
 Microbacterium sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.20.4.1 glutaredoxin
-
 Synechocystis sp.
1.20.4.1 glutathione
-
 Synechocystis sp.

Expression

EC Number Organism Comment Expression
1.20.4.4 Microbacterium sp. arsenate up-regulates expression up

General Information

EC Number General Information Comment Organism
1.20.4.4 malfunction ArsC3 together with ArsC1 is able to rescue the arsenate sensitivity phenotype of Escherichia coli mutant AW3110 Microbacterium sp.
1.20.4.4 malfunction ArsC3 together with ArsC1 is able to rescue the arsenate sensitivity phenotype of Escherichia coli mutant AW3110. ArsC1 is the major contributor of arsenate resistance in Escherichia coli Microbacterium sp.
1.20.4.4 physiological function ArsC1 functions as an arsenate reductase required for As(V) detoxification Microbacterium sp.
1.20.4.4 physiological function ArsC1 is part of a gene cluster consisting of pair of genes (arsTX) encoding a thioredoxin system that are cotranscribed with an unusual arsRC2 fusion gene, ACR3, and arsC1 in an operon divergent from arsC3. The whole arsenic resistance system gene cluster is required to fully complement an Escherichia coli ars mutant AW3110 (strain lacking the arsenic resistance system operon) Microbacterium sp.
1.20.4.4 physiological function ArsC3 functions as arsenate reductase required for As(V) detoxification Microbacterium sp.
1.20.4.4 physiological function ArsC3 is part of a gene cluster consisting of pair of genes (arsTX) encoding a thioredoxin system that are cotranscribed with an unusual arsRC2 fusion gene, ACR3, and arsC1 in an operon divergent from arsC3. The whole arsenic resistance system gene cluster is required to fully complement an Escherichia coli ars mutant AW3110 (strain lacking the arsenic resistance system operon) Microbacterium sp.