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Literature summary extracted from

  • Basu, S.; Roy, A.; Ghosh, A.; Bera, A.; Chattopadhyay, D.; Chakrabarti, K.
    Arg235 is an essential catalytic residue of Bacillus pumilus DKS1 pectate lyase to degum ramie fibre (2011), Biodegradation, 22, 153-161.
    View publication on PubMed

Application

EC Number Application Comment Organism
4.2.2.2 industry the thermoactive pectate lyase from strain DKS1 of Bacillus pumilus has a potential use for degumming ramie fiber, the purified pectate lyase causes a weight loss of the ramie fibre of 25%. Arg235 is an essential catalytic residue to degumming ramie fibre Bacillus pumilus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.2.2 gene pelB, DNA and amino acid sequence determination and analysis, expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) plysS Bacillus pumilus

Protein Variants

EC Number Protein Variants Comment Organism
4.2.2.2 R235A site-directed mutagenesis, inactive catalytic residue mutant Bacillus pumilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.2.2 Ca2+ activates Bacillus pumilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.2 37556
-
x * 37556, sequence calculation Bacillus pumilus

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.2 Bacillus pumilus B2ZFM0 gene pelB
-
4.2.2.2 Bacillus pumilus DKS1 B2ZFM0 gene pelB
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.2 recombinant enzyme from Escherichia coli strain BL21(DE3) plysS 6.64fold by single step cation exchange chromatography to homogeneity Bacillus pumilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.2.2 additional information
-
93 U/ml and weight of the ramie fibre of 1 g, pH 8.5, 37°C Bacillus pumilus
4.2.2.2 2893
-
purified recombinant wild-type enzyme, pH 8.5, 60°C Bacillus pumilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.2 polygalacturonic acid Arg235 is an essential catalytic residue Bacillus pumilus ?
-
?
4.2.2.2 polygalacturonic acid Arg235 is an essential catalytic residue Bacillus pumilus DKS1 ?
-
?

Subunits

EC Number Subunits Comment Organism
4.2.2.2 ? x * 37556, sequence calculation Bacillus pumilus

Synonyms

EC Number Synonyms Comment Organism
4.2.2.2 DKS1 pectate lyase
-
Bacillus pumilus
4.2.2.2 More the enzyme belongs to the polysaccharide family 1 Bacillus pumilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.2 60
-
-
Bacillus pumilus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.2.2.2 30 100 activity range Bacillus pumilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.2 8.5
-
-
Bacillus pumilus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.2.2 3 11
-
Bacillus pumilus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.2.2.2 Bacillus pumilus sequence calculation
-
9.23