Literature summary extracted from
Basu, S.; Roy, A.; Ghosh, A.; Bera, A.; Chattopadhyay, D.; Chakrabarti, K.
Arg235 is an essential catalytic residue of Bacillus pumilus DKS1 pectate lyase to degum ramie fibre (2011), Biodegradation, 22, 153-161.
Application
EC Number |
Application |
Comment |
Organism |
---|
4.2.2.2 |
industry |
the thermoactive pectate lyase from strain DKS1 of Bacillus pumilus has a potential use for degumming ramie fiber, the purified pectate lyase causes a weight loss of the ramie fibre of 25%. Arg235 is an essential catalytic residue to degumming ramie fibre |
Bacillus pumilus |
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
4.2.2.2 |
gene pelB, DNA and amino acid sequence determination and analysis, expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) plysS |
Bacillus pumilus |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
4.2.2.2 |
R235A |
site-directed mutagenesis, inactive catalytic residue mutant |
Bacillus pumilus |
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
4.2.2.2 |
Ca2+ |
activates |
Bacillus pumilus |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
4.2.2.2 |
37556 |
- |
x * 37556, sequence calculation |
Bacillus pumilus |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
4.2.2.2 |
Bacillus pumilus |
B2ZFM0 |
gene pelB |
- |
4.2.2.2 |
Bacillus pumilus DKS1 |
B2ZFM0 |
gene pelB |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
4.2.2.2 |
recombinant enzyme from Escherichia coli strain BL21(DE3) plysS 6.64fold by single step cation exchange chromatography to homogeneity |
Bacillus pumilus |
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
4.2.2.2 |
additional information |
- |
93 U/ml and weight of the ramie fibre of 1 g, pH 8.5, 37°C |
Bacillus pumilus |
4.2.2.2 |
2893 |
- |
purified recombinant wild-type enzyme, pH 8.5, 60°C |
Bacillus pumilus |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
4.2.2.2 |
polygalacturonic acid |
Arg235 is an essential catalytic residue |
Bacillus pumilus |
? |
- |
? |
|
4.2.2.2 |
polygalacturonic acid |
Arg235 is an essential catalytic residue |
Bacillus pumilus DKS1 |
? |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
4.2.2.2 |
? |
x * 37556, sequence calculation |
Bacillus pumilus |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
4.2.2.2 |
DKS1 pectate lyase |
- |
Bacillus pumilus |
4.2.2.2 |
More |
the enzyme belongs to the polysaccharide family 1 |
Bacillus pumilus |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
4.2.2.2 |
60 |
- |
- |
Bacillus pumilus |
Temperature Range [°C]
EC Number |
Temperature Minimum [°C] |
Temperature Maximum [°C] |
Comment |
Organism |
---|
4.2.2.2 |
30 |
100 |
activity range |
Bacillus pumilus |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
4.2.2.2 |
8.5 |
- |
- |
Bacillus pumilus |
pH Range
EC Number |
pH Minimum |
pH Maximum |
Comment |
Organism |
---|
4.2.2.2 |
3 |
11 |
- |
Bacillus pumilus |
pI Value
EC Number |
Organism |
Comment |
pI Value Maximum |
pI Value |
---|
4.2.2.2 |
Bacillus pumilus |
sequence calculation |
- |
9.23 |