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Literature summary extracted from

  • May, A.; Spinka, M.; Koeck, M.
    Arabidopsis thaliana PECP1 - Enzymatic characterization and structural organization of the first plant phosphoethanolamine/phosphocholine phosphatase (2012), Biochim. Biophys. Acta, 1824, 319-325.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.75 expressed in Escherichia coli BL21 Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.75 0.44
-
 phosphocholine 50 mM Hepes/NaOH buffer, pH 7.0, 37°C Arabidopsis thaliana
3.1.3.75 1.16
-
 Phosphoethanolamine 50 mM Hepes/NaOH buffer, pH 7.0, 37°C Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.75 cytosol
-
 Arabidopsis thaliana 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.75 Co2+ divalent metal ions required, highest activity with 1.5 mM Mg2+ Arabidopsis thaliana
3.1.3.75 Fe2+ divalent metal ions required, highest activity with 1.5 mM Mg2+ Arabidopsis thaliana
3.1.3.75 Mg2+ divalent metal ions required, highest activity with 1.5 mM Mg2+ Arabidopsis thaliana
3.1.3.75 additional information not activated by Ca2+, Zn2+, or Cu2+ Arabidopsis thaliana
3.1.3.75 Ni2+ divalent metal ions required, highest activity with 1.5 mM Mg2+ Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.75 31570
-
 calculated from amino acid sequence Arabidopsis thaliana
3.1.3.75 32000
-
 gel filtration, SDS-PAGE Arabidopsis thaliana
3.1.3.75 64000
-
 native gel elelctrophoresis, gel filtration Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.75 phosphocholine + H2O Arabidopsis thaliana
-
 choline + phosphate
-
 ?
3.1.3.75 phosphoethanolamine + H2O Arabidopsis thaliana
-
 ethanolamine + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.75 Arabidopsis thaliana Q9FZ62
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.75 Ni2+-Sepharose activated column Arabidopsis thaliana

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.75 0.48
-
 substrate phosphocholine, 50 mM Hepes/NaOH buffer, pH 7.0, 37°C Arabidopsis thaliana
3.1.3.75 3.67
-
 substrate phosphoethanolamine, 50 mM Hepes/NaOH buffer, pH 7.0, 37°C Arabidopsis thaliana

Storage Stability

EC Number Storage Stability Organism
3.1.3.75 -80°C Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.75 additional information no substrates are 4-nitrophenyl phosphate, diphosphate, phospho-L-serine, phospho-L-tyrosine Arabidopsis thaliana ?
-
 ?
3.1.3.75 phosphocholine + H2O
-
 Arabidopsis thaliana choline + phosphate
-
 ?
3.1.3.75 phosphoethanolamine + H2O
-
 Arabidopsis thaliana ethanolamine + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.75 dimer 2 * 32000, monomer-dimer equilibrium, both the monomer and the dimer are active Arabidopsis thaliana
3.1.3.75 monomer 1 * 32000, monomer-dimer equilibrium, both the monomer and the dimer are active Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
3.1.3.75 AtPECP1
-
 Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.75 1.04
-
 phosphocholine 50 mM Hepes/NaOH buffer, pH 7.0, 37°C Arabidopsis thaliana
3.1.3.75 13
-
 Phosphoethanolamine 50 mM Hepes/NaOH buffer, pH 7.0, 37°C Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.75 6.2 7.8
-
 Arabidopsis thaliana

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.3.75 Arabidopsis thaliana theoretical value
-
 5

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.3.75 1.06
-
 Phosphoethanolamine 50 mM Hepes/NaOH buffer, pH 7.0, 37°C Arabidopsis thaliana
3.1.3.75 2.34
-
 phosphocholine 50 mM Hepes/NaOH buffer, pH 7.0, 37°C Arabidopsis thaliana