Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Chandra, D.; Prasanth, G.; Singh, N.; Kumar, S.; Jithesh, O.; Sadasivan, C.; Sharma, S.; Singh, T.; Haridas, M.
    Identification of a novel and potent inhibitor of phospholipase A 2 in a medicinal plant: Crystal structure at 1.93 A and Surface Plasmon Resonance analysis of phospholipase A2 complexed with berberine (2011), Biochim. Biophys. Acta, 1814, 657-663.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.1.4 phospholipase A2 complexed with berberine, hanging drop vapor diffusion method, using 5 mM CaCl2 in 25 mM sodium acetate buffer of pH 6.2 with 15% (w/v) polyethylene glycol 8000 and 0.2 M ammonium sulfate Daboia russelii russelii

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.4 ajmaline
-
 Daboia russelii russelii
3.1.1.4 Anisic acid
-
 Daboia russelii russelii
3.1.1.4 aristolochic acid
-
 Daboia russelii russelii
3.1.1.4 Berberine competitive inhibitor of phospholipase A2 Daboia russelii russelii
3.1.1.4 diclofenac
-
 Daboia russelii russelii
3.1.1.4 oxyphenbutazone
-
 Daboia russelii russelii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.4 0.02
-
 soya lecithin at pH 8.9, temperature not specified in the publication Daboia russelii russelii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.4 soya lecithin + H2O Daboia russelii russelii
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.4 Daboia russelii russelii P59071 Indian Russell's viper, chain viper
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.4 venom
-
 Daboia russelii russelii
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.4 soya lecithin + H2O
-
 Daboia russelii russelii ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.4 PLA2
-
 Daboia russelii russelii

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.1.4 0.021
-
 Berberine at pH 8.9, temperature not specified in the publication Daboia russelii russelii

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.1.1.4 0.087
-
 at pH 8.9, temperature not specified in the publication Daboia russelii russelii Berberine