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Literature summary extracted from

  • Tanabe, Y.; Oda, M.
    Molecular characterization of endo-1,3-beta-glucanase from Cellulosimicrobium cellulans: Effects of carbohydrate-binding module on enzymatic function and stability (2011), Biochim. Biophys. Acta, 1814, 1713-1719.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.39 expression of catalytic domain Ala1-Thr256 in Escherichia coli Cellulosimicrobium cellulans

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.39 E119A variant of catalytic domain, significant decrease in hydrolytic activity for laminarin Cellulosimicrobium cellulans
3.2.1.39 E119A/Ldel variant of catalytic domain with deletion of the Gly/Ser-rich linker, Ser244-Thr256, no expression of protein Cellulosimicrobium cellulans
3.2.1.39 M123del variant of catalytic domain, significant decrease in hydrolytic activity for laminarin Cellulosimicrobium cellulans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.39 0.2
-
 Laminariheptaose recombinant catalytic domain, pH 7.0, 25°C Cellulosimicrobium cellulans
3.2.1.39 0.32
-
 Laminaripentaose native enzyme, pH 7.0, 25°C Cellulosimicrobium cellulans
3.2.1.39 0.36
-
 Laminaripentaose recombinant catalytic domain, pH 7.0, 25°C Cellulosimicrobium cellulans
3.2.1.39 0.75
-
 Laminariheptaose native enzyme, pH 7.0, 25°C Cellulosimicrobium cellulans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.39 40798
-
 x * 41000, SDS-PAGE, x * 40798, MALDI-TOF Cellulosimicrobium cellulans
3.2.1.39 41000
-
 x * 41000, SDS-PAGE, x * 40798, MALDI-TOF Cellulosimicrobium cellulans

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.39 Cellulosimicrobium cellulans
-
 enzyme comprises a catalytic domain of the glycoside hydrolase family 16 and a C-terminal carbohydrate-binding module family 13
-
3.2.1.39 Cellulosimicrobium cellulans DK-1
-
 enzyme comprises a catalytic domain of the glycoside hydrolase family 16 and a C-terminal carbohydrate-binding module family 13
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.39 both native enzyme and recombinant catalytic domain Cellulosimicrobium cellulans

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.39 commercial preparation Tunicase, a crude enzyme preparation from Cellulosimicrobium cellulans DK-1 Cellulosimicrobium cellulans
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.39 8
-
 recombinant catalytic domain, pH 7.0, 25°C Cellulosimicrobium cellulans
3.2.1.39 8.47
-
 native enzyme, pH 7.0, 25°C Cellulosimicrobium cellulans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.39 laminariheptaose + H2O
-
 Cellulosimicrobium cellulans ?
-
 ?
3.2.1.39 laminariheptaose + H2O
-
 Cellulosimicrobium cellulans DK-1 ?
-
 ?
3.2.1.39 laminarin + H2O
-
 Cellulosimicrobium cellulans ?
-
 ?
3.2.1.39 laminarin + H2O
-
 Cellulosimicrobium cellulans DK-1 ?
-
 ?
3.2.1.39 laminaripentaose + H2O
-
 Cellulosimicrobium cellulans ?
-
 ?
3.2.1.39 laminaripentaose + H2O
-
 Cellulosimicrobium cellulans DK-1 ?
-
 ?
3.2.1.39 additional information no substrate: laminaribiose or laminaritriose, carboxymethyl cellulose, chitin, and pustulan Cellulosimicrobium cellulans ?
-
 ?
3.2.1.39 additional information no substrate: laminaribiose or laminaritriose, carboxymethyl cellulose, chitin, and pustulan Cellulosimicrobium cellulans DK-1 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.39 ? x * 41000, SDS-PAGE, x * 40798, MALDI-TOF Cellulosimicrobium cellulans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.39 70
-
 10 min, complete loss of activity both for catalytic domain and native enzyme Cellulosimicrobium cellulans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.39 30
-
 Laminariheptaose recombinant catalytic domain, pH 7.0, 25°C Cellulosimicrobium cellulans
3.2.1.39 42
-
 Laminaripentaose recombinant catalytic domain, pH 7.0, 25°C Cellulosimicrobium cellulans
3.2.1.39 69
-
 Laminariheptaose native enzyme, pH 7.0, 25°C Cellulosimicrobium cellulans
3.2.1.39 75
-
 Laminaripentaose native enzyme, pH 7.0, 25°C Cellulosimicrobium cellulans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.39 3 10 native enzyme Cellulosimicrobium cellulans
3.2.1.39 4
-
 loss of activity below, recombinant catalytic domain Cellulosimicrobium cellulans

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.39 93
-
 Laminariheptaose native enzyme, pH 7.0, 25°C Cellulosimicrobium cellulans
3.2.1.39 118
-
 Laminaripentaose recombinant catalytic domain, pH 7.0, 25°C Cellulosimicrobium cellulans
3.2.1.39 156
-
 Laminariheptaose recombinant catalytic domain, pH 7.0, 25°C Cellulosimicrobium cellulans
3.2.1.39 237
-
 Laminaripentaose native enzyme, pH 7.0, 25°C Cellulosimicrobium cellulans