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Literature summary extracted from

  • Marcia, M.; Langer, J.D.; Parcej, D.; Vogel, V.; Peng, G.; Michel, H.
    Characterizing a monotopic membrane enzyme. Biochemical, enzymatic and crystallization studies on Aquifex aeolicus sulfide:quinone oxidoreductase (2010), Biochim. Biophys. Acta, 1798, 2114-2123.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.8.5.4
-
 Aquifex aeolicus
1.8.5.4 crystallization in two crystal forms of hexagonal, prism shape and thin, elongated shape Aquifex aeolicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.5.4 Antimycin competitive Aquifex aeolicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.5.4 0.0016
-
 ubiquinone-4 pH 7.4, temperature not specified in the publication Aquifex aeolicus
1.8.5.4 0.0016
-
 ubiquinone-4 in 50 mM Tris-HCl, pH 7.4, 40°C Aquifex aeolicus
1.8.5.4 0.00216
-
 decylubiquinone pH 7.4, temperature not specified in the publication Aquifex aeolicus
1.8.5.4 0.00216
-
 decylubiquinone in 50 mM Tris-HCl, pH 7.4, 40°C Aquifex aeolicus
1.8.5.4 0.0054
-
 ubiquinone-1 pH 7.4, temperature not specified in the publication Aquifex aeolicus
1.8.5.4 0.0054
-
 ubiquinone-1 in 50 mM Tris-HCl, pH 7.4, 40°C Aquifex aeolicus
1.8.5.4 0.00594
-
 Sulfide pH 7.4, temperature not specified in the publication Aquifex aeolicus
1.8.5.4 0.00594
-
 Sulfide in 50 mM Tris-HCl, pH 7.4, 40°C Aquifex aeolicus
1.8.5.4 0.00643
-
 ubiquinone-9 pH 7.4, temperature not specified in the publication Aquifex aeolicus
1.8.5.4 0.00643
-
 ubiquinone-9 in 50 mM Tris-HCl, pH 7.4, 40°C Aquifex aeolicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.8.5.4 membrane monotopic membrane enzyme Aquifex aeolicus 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.5.4 16000
-
 sedimentation equilibrium centrifugation Aquifex aeolicus
1.8.5.4 47000
-
 x * 47000, SDS-PAGE Aquifex aeolicus
1.8.5.4 47000
-
 3 * 47000, SDS-PAGE Aquifex aeolicus
1.8.5.4 120000
-
 gel filtration Aquifex aeolicus
1.8.5.4 160000
-
 analytical ultracentrifugation Aquifex aeolicus

Organism

EC Number Organism UniProt Comment Textmining
1.8.5.4 Aquifex aeolicus
-
-
-
1.8.5.4 Aquifex aeolicus O67931
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.5.4 MonoQ column chromatography, TSK-1 gel filtration, and TSK-2 gel filtration Aquifex aeolicus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.5.4 71.4
-
 at 40°C, pH 7.4 Aquifex aeolicus

Storage Stability

EC Number Storage Stability Organism
1.8.5.4 4C, protein is stable and monodisperse in 50 mM dodecyl-D-maltoside for weeks, either in the absence of salt or in the presence of N 1 M NaCl Aquifex aeolicus
1.8.5.4 4°C, purified protein in 3% (w/v) dodecyl-beta-D-maltoside either in the absence of salt or in the presence of N 1 M NaCl, six weeks, no loss of activity Aquifex aeolicus
1.8.5.4 80°C, purified protein in 3% (w/v) dodecyl-beta-D-maltoside either in the absence of salt or in the presence of N 1 M NaCl, one day, 50% loss of activity Aquifex aeolicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.5.4 sulfide + decylubiquinone
-
 Aquifex aeolicus sulfur + decylubiquinol
-
 ?
1.8.5.4 sulfide + ubiquinone-1
-
 Aquifex aeolicus sulfur + ubiquinol-1
-
 ?
1.8.5.4 sulfide + ubiquinone-4
-
 Aquifex aeolicus sulfur + ubiquinol-4
-
 ?
1.8.5.4 sulfide + ubiquinone-9
-
 Aquifex aeolicus sulfur + ubiquinol-9
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.5.4 homotrimer 3 * 47000, SDS-PAGE Aquifex aeolicus
1.8.5.4 multimer x * 47000, SDS-PAGE Aquifex aeolicus

Synonyms

EC Number Synonyms Comment Organism
1.8.5.4 sulfide:quinone oxidoreductase
-
 Aquifex aeolicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.8.5.4 80
-
-
 Aquifex aeolicus
1.8.5.4 80
-
 the activity is highest at 80°C (the highest temperature tested) Aquifex aeolicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.8.5.4 80
-
 the half-life at 80°C is 32 h Aquifex aeolicus
1.8.5.4 80
-
 50% loss of activity after one day Aquifex aeolicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.5.4 6.5
-
-
 Aquifex aeolicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.5.4 FAD one FAD molecule per protein monomer Aquifex aeolicus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.8.5.4 0.015
-
 in 50 mM Tris-HCl, pH 7.4, 40°C Aquifex aeolicus Antimycin