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Literature summary extracted from

  • Umbach, A.L.; Gonzalez-Meler, M.A.; Sweet, C.R.; Siedow, J.N.
    Activation of the plant mitochondrial alternative oxidase: insights from site-directed mutagenesis (2002), Biochim. Biophys. Acta, 1554, 118-128.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.10.3.11 pyruvate the wild type enzyme activity is significantly stimulated (122%) by 5 mM pyruvate Arabidopsis thaliana

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.10.3.11 expressed in Escherichia coli SASX41B cells Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
1.10.3.11 C78A the mutant shows significant (418%) stimulation by 5 mM succinate and little response to 5 mM pyruvate Arabidopsis thaliana
1.10.3.11 C78D the mutant is significantly stimulated (28%) by 5 mM succinate, pyruvate has no significant effect on the mutant enzyme activity Arabidopsis thaliana
1.10.3.11 C78E the mutant is significantly stimulated (37%) by 5 mM succinate Arabidopsis thaliana
1.10.3.11 C78K the mutant is insensitive to pyruvate or succinate but more active than the wild type without pyruvate Arabidopsis thaliana
1.10.3.11 C78L inactive Arabidopsis thaliana
1.10.3.11 C78Q inactive Arabidopsis thaliana
1.10.3.11 C78R the mutant is insensitive to pyruvate or succinate but more active than the wild type without pyruvate Arabidopsis thaliana
1.10.3.11 C78S the mutant is significantly (489%) stimulated by 5 mM succinate Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.11 Salicylhydroxamic acid
-
 Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.10.3.11 mitochondrion
-
 Arabidopsis thaliana 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.11 29000
-
 2 * 29000, SDS-PAGE Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.11 Arabidopsis thaliana
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.11
-
 Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.11 duroquinol + O2
-
 Arabidopsis thaliana duroquinone + H2O
-
 ?
1.10.3.11 NADH + O2
-
 Arabidopsis thaliana ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.10.3.11 homodimer 2 * 29000, SDS-PAGE Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
1.10.3.11 AOX1a
-
 Arabidopsis thaliana
1.10.3.11 cyanide-resistant alternative oxidase
-
 Arabidopsis thaliana