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Literature summary extracted from

  • Berthold, D.A.
    Isolation of mutants of the Arabidopsis thaliana alternative oxidase (ubiquinol:oxygen oxidoreductase) resistant to salicylhydroxamic acid (1998), Biochim. Biophys. Acta, 1364, 73-83.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.10.3.11 pyruvate the alternative oxidase is 50% activated at a concentration of 0.1 mM pyruvate Arabidopsis thaliana

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.10.3.11 mutant enzymes are expressed in heme-deficient Escherichia coli SASX41B cells Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
1.10.3.11 F215L the mutant exhibits 1.6fold resistance to salicylhydroxamic acid compared to the wild type enzyme Arabidopsis thaliana
1.10.3.11 G303E the mutant exhibits 4.6fold resistance to salicylhydroxamic acid compared to the wild type enzyme Arabidopsis thaliana
1.10.3.11 M219I the mutant exhibits 1.4fold resistance to salicylhydroxamic acid compared to the wild type enzyme Arabidopsis thaliana
1.10.3.11 M219V the mutant exhibits 1.7fold resistance to salicylhydroxamic acid compared to the wild type enzyme Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.11 propyl gallate
-
 Arabidopsis thaliana
1.10.3.11 Salicylhydroxamic acid
-
 Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.10.3.11 mitochondrial membrane
-
 Arabidopsis thaliana 31966
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.10.3.11 Fe2+ the enzyme contains non-heme iron at its active site Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.11 Arabidopsis thaliana
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.11 duroquinol + O2
-
 Arabidopsis thaliana duroquinone + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.10.3.11 alternative oxidase
-
 Arabidopsis thaliana
1.10.3.11 ubiquinol:oxygen oxidoreductase
-
 Arabidopsis thaliana

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.10.3.11 0.00036
-
 mutant enzyme F215L, in 50 mM potassium phosphate, 10 mM KCl, 5 mM MgCl , 1 mM 2 EDTA, 5 mM pyruvate, pH 7.0, at 25°C Arabidopsis thaliana propyl gallate
1.10.3.11 0.00039
-
 mutant enzyme M219V, in 50 mM potassium phosphate, 10 mM KCl, 5 mM MgCl , 1 mM 2 EDTA, 5 mM pyruvate, pH 7.0, at 25°C Arabidopsis thaliana propyl gallate
1.10.3.11 0.00039
-
 wild type enzyme, in 50 mM potassium phosphate, 10 mM KCl, 5 mM MgCl , 1 mM 2 EDTA, 5 mM pyruvate, pH 7.0, at 25°C Arabidopsis thaliana propyl gallate
1.10.3.11 0.00042
-
 mutant enzyme M219I, in 50 mM potassium phosphate, 10 mM KCl, 5 mM MgCl , 1 mM 2 EDTA, 5 mM pyruvate, pH 7.0, at 25°C Arabidopsis thaliana propyl gallate
1.10.3.11 0.00044
-
 mutant enzyme G303E, in 50 mM potassium phosphate, 10 mM KCl, 5 mM MgCl , 1 mM 2 EDTA, 5 mM pyruvate, pH 7.0, at 25°C Arabidopsis thaliana propyl gallate
1.10.3.11 0.042
-
 wild type enzyme, in 50 mM potassium phosphate, 10 mM KCl, 5 mM MgCl , 1 mM 2 EDTA, 5 mM pyruvate, pH 7.0, at 25°C Arabidopsis thaliana Salicylhydroxamic acid
1.10.3.11 0.057
-
 mutant enzyme M219I, in 50 mM potassium phosphate, 10 mM KCl, 5 mM MgCl , 1 mM 2 EDTA, 5 mM pyruvate, pH 7.0, at 25°C Arabidopsis thaliana Salicylhydroxamic acid
1.10.3.11 0.069
-
 mutant enzyme F215L, in 50 mM potassium phosphate, 10 mM KCl, 5 mM MgCl , 1 mM 2 EDTA, 5 mM pyruvate, pH 7.0, at 25°C Arabidopsis thaliana Salicylhydroxamic acid
1.10.3.11 0.073
-
 mutant enzyme M219V, in 50 mM potassium phosphate, 10 mM KCl, 5 mM MgCl , 1 mM 2 EDTA, 5 mM pyruvate, pH 7.0, at 25°C Arabidopsis thaliana Salicylhydroxamic acid
1.10.3.11 0.194
-
 mutant enzyme G303E, in 50 mM potassium phosphate, 10 mM KCl, 5 mM MgCl , 1 mM 2 EDTA, 5 mM pyruvate, pH 7.0, at 25°C Arabidopsis thaliana Salicylhydroxamic acid