Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Hayashi, M.; Ohzeki, H.; Shimada, H.; Unemoto, T.
    NADPH-specific quinone reductase is induced by 2-methylene-4-butyrolactone in Escherichia coli (1996), Biochim. Biophys. Acta, 1273, 165-170.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.6.5.10 2-methylene-4-butyrolactone 25fold induction Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.6.5.10
-
 Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.6.5.10 additional information insensitive to 4-chloromercuribenzoate at 100 microM, heavy metals such as Cu2+, Co2+, Cd2+ and Mn2+ at 10 microM each, and chelating agents such as EDTA, o-phenanthroline and diethyldithiocarbamate at 100 microM each Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.5.10 0.006
-
 menadione pH 7.5, 30°C Escherichia coli
1.6.5.10 0.0105
-
 NADPH pH 7.5, 30°C Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.6.5.10 soluble
-
 Escherichia coli
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.6.5.10 additional information
-
 enzyme displays three charge isomers with different relative mobilities Escherichia coli
1.6.5.10 21000
-
 2 * 21000, SDS-PAGE Escherichia coli
1.6.5.10 42000
-
 PAGE Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.6.5.10 Escherichia coli
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.6.5.10 1067
-
 pH 7.5, 30°C Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.5.10 1,4-benzoquinone + NADPH + H+ about 81% of the activity with menadione Escherichia coli 1,4-benzoquinol + NADP+
-
 ?
1.6.5.10 1,4-naphthoquinone + NADPH + H+ about 85% of the activity with menadione Escherichia coli 1,4-naphthoquinol + NADP+
-
 ?
1.6.5.10 2 ferricyanide + NADPH about 90% of the activity with menadione Escherichia coli 2 ferrocyanide + NADP+ + H+
-
 ?
1.6.5.10 dichlorophenolindophenol + NADPH + H+ about 15% of the activity with menadione Escherichia coli ? + NADP+
-
 ?
1.6.5.10 menadione + NADPH + H+ reduction follows a two-electron-pathway Escherichia coli menadiol + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.6.5.10 dimer 2 * 21000, SDS-PAGE Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.6.5.10 7
-
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.5.10 FAD enzyme displays flavin absorption spectrum with peaks at 376 and 456 nm and a shoulder at around 484 nm. Protein caontains FAD Escherichia coli
1.6.5.10 additional information no cofactor: NADH, FMN Escherichia coli
1.6.5.10 NADPH specific Escherichia coli