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Literature summary extracted from

  • Cendrin, F.; Jouve, H.M.; Gaillard, J.; Thibault, P.; Zaccai, G.
    Purification and properties of a halophilic catalase-peroxidase from Haloarcula marismortui (1994), Biochim. Biophys. Acta, 1209, 1-9.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.11.1.21 ammonium sulfate additions of ammonium sulfate even at weak concentrations (10 to 70 mM) stimulate catalatic activity measured in the presence of 1.5 M NaC1 by about 30% Haloarcula marismortui
1.11.1.21 NaCl with NaC1, maximum activity is observed near 0.5-0.7 M Haloarcula marismortui

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.11.1.21 additional information no inhibition of the catalase activity of hCP is seen with 3-amino-l,2,4-triazole Haloarcula marismortui
1.11.1.21 NaCl the peroxidatic activity on diaminobenzidine shows a continuous decrease with increasing NaCl concentration Haloarcula marismortui

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.21 0.5
-
 H2O2 peroxidatic activity, pH 6.0, 25°C Haloarcula marismortui
1.11.1.21 2.5
-
 H2O2 catalatic activity, pH 7.5, 25°C Haloarcula marismortui

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.21 81290
-
 mass spectrometry Haloarcula marismortui
1.11.1.21 94000
-
 SDS-PAGE Haloarcula marismortui

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.21 Haloarcula marismortui
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.21 Sepharose column chromatography, DEAE cellulose column chromatography, dialysis, hydroxyapatite column chromatography, and ACA 44 gel filtration Haloarcula marismortui

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.11.1.21 5
-
 crude extract, catalatic activity, at 20°C and pH 7.5, in the presence of 1.5 M NaC1 Haloarcula marismortui
1.11.1.21 851
-
 after 170fold purification, catalatic activity, at 20°C and pH 7.5, in the presence of 1.5 M NaC1 Haloarcula marismortui

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.21 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2
-
 Haloarcula marismortui oxidized 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O
-
 ?
1.11.1.21 diaminobenzidine + H2O2
-
 Haloarcula marismortui oxidized diaminobenzidine + H2O
-
 ?
1.11.1.21 guaiacol + H2O2
-
 Haloarcula marismortui tetraguaiacol + H2O
-
 ?
1.11.1.21 H2O2
-
 Haloarcula marismortui O2 + H2O
-
 ?
1.11.1.21 o-dianisidine + H2O2
-
 Haloarcula marismortui oxidized o-dianisidine + H2O
-
 ?
1.11.1.21 pyrogallol + H2O2
-
 Haloarcula marismortui ? + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.11.1.21 HCP
-
 Haloarcula marismortui

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.21 6
-
 peroxidatic activity in the absence of NaCl, with diaminobenzidine as electron donor Haloarcula marismortui
1.11.1.21 7.5
-
 catalatic activity in molar concentrations of NaCl, with diaminobenzidine as electron donor Haloarcula marismortui

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.1.21 heme
-
 Haloarcula marismortui