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Literature summary extracted from

  • Pintus, F.; Spano, D.; Corongiu, S.; Floris, G.; Medda, R.
    Purification, primary structure, and properties of Euphorbia characias latex purple acid phosphatase (2011), Biochemistry, 76, 694-701.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.2
-
 Euphorbia characias

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.2 molybdate inhibits enzymatic activity Euphorbia characias
3.1.3.2 vanadate inhibits enzymatic activity Euphorbia characias
3.1.3.2 Zn2+ inhibits enzymatic activity Euphorbia characias

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.2 0.51
-
 4-nitrophenyl phosphate pH 5.75, 25°C Euphorbia characias

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.2 Fe ELPAP contains 1 mol of Fe and 1 mole of Zn per mol of subunit Euphorbia characias
3.1.3.2 Zn ELPAP contains 1 mol of Fe and 1 mole of Zn per mol of subunit Euphorbia characias

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.2 68000
-
 SDS-PAGE Euphorbia characias
3.1.3.2 130000
-
 native protein, gel filtration Euphorbia characias

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.2 Euphorbia characias
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.2 using ammonium sulfate fractionation, DEAE-cellulose chromatography and gel filtration chromatography Euphorbia characias

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.2 2,3-bisphosphoglycerate + H2O 85% hydrolysis compared to hydrolysis of 4-nitrophenyl phosphate Euphorbia characias ?
-
 ?
3.1.3.2 4-nitrophenyl phosphate + H2O
-
 Euphorbia characias 4-nitrophenol + phosphate
-
 ?
3.1.3.2 ADP + H2O 100% hydrolysis compared to hydrolysis of 4-nitrophenyl phosphate Euphorbia characias AMP + phosphate
-
 ?
3.1.3.2 AMP + H2O 16% hydrolysis compared to hydrolysis of 4-nitrophenyl phosphate Euphorbia characias adenosine + phosphate
-
 ?
3.1.3.2 ATP + H2O 100% hydrolysis compared to hydrolysis of 4-nitrophenyl phosphate Euphorbia characias ADP + phosphate
-
 ?
3.1.3.2 D-glucose 1-phosphate + H2O 25% hydrolysis compared to hydrolysis of 4-nitrophenyl phosphate Euphorbia characias D-glucose + phosphate
-
 ?
3.1.3.2 D-glucose 6-phosphate + H2O 104% hydrolysis compared to hydrolysis of 4-nitrophenyl phosphate Euphorbia characias D-glucose + phosphate
-
 ?
3.1.3.2 GMP + H2O 66% hydrolysis compared to hydrolysis of 4-nitrophenyl phosphate Euphorbia characias guanosine + phosphate
-
 ?
3.1.3.2 inositol 1-phosphate + H2O 60% hydrolysis compared to hydrolysis of 4-nitrophenyl phosphate Euphorbia characias inositol + phosphate
-
 ?
3.1.3.2 phosphoenolpyruvate + H2O 96% hydrolysis compared to hydrolysis of 4-nitrophenyl phosphate Euphorbia characias pyruvate + phosphate
-
 ?
3.1.3.2 pyridoxal 5'-phosphate + H2O 80% hydrolysis compared to hydrolysis of 4-nitrophenyl phosphate Euphorbia characias pyridoxal + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.2 homodimer each subunit contain one Fe(III) and one Zn(II) ion. The two subunits are connected by a disulfide bridge. 2 * 68000, SDS-PAGE Euphorbia characias

Synonyms

EC Number Synonyms Comment Organism
3.1.3.2 ELPAP
-
 Euphorbia characias
3.1.3.2 latex purple acid phosphatase
-
 Euphorbia characias

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.2 25
-
 standard assay at Euphorbia characias

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.2 50 60 enzyme is stable at 50°C but loses 70% of its activity at 60°C Euphorbia characias

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.2 790
-
 4-nitrophenyl phosphate pH 5.75, 25°C Euphorbia characias

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.2 5.75
-
-
 Euphorbia characias

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.3.2 0.0001
-
 vanadate pH 5.75, 25°C Euphorbia characias
3.1.3.2 0.0008
-
 molybdate pH 5.75, 25°C Euphorbia characias