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Literature summary extracted from

  • Li, H.J.; Li, X.; Liu, N.; Zhang, H.; Truglio, J.J.; Mishra, S.; Kisker, C.F.; Garcia-Diaz, M.; Tonge, P.J.
    Mechanism of the intramolecular Claisen condensation reaction catalyzed by MenB, a crotonase superfamily member (2011), Biochemistry, 50, 9532-9544.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.3.36 expressed in Escherichia coli BL21(DE3) Mycobacterium tuberculosis
4.1.3.36 His-tagged version expressed in Escherichia coli BL21(DE3) Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.3.36 hanging drop vapor diffusion technique, PEG 6000, in complex with o-succinylbenzoyl-aminoCoA Mycobacterium tuberculosis
4.1.3.36 sitting drop vapor diffusion technique, PEG 3350, in complex with o-succinylbenzoyl-amino-CoA Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
4.1.3.36 D185E reduced kcat Mycobacterium tuberculosis
4.1.3.36 D185G no activity Mycobacterium tuberculosis
4.1.3.36 D185N reduced kcat Mycobacterium tuberculosis
4.1.3.36 D192N no activity Mycobacterium tuberculosis
4.1.3.36 G156D no activity Escherichia coli
4.1.3.36 S190A reduced kcat Mycobacterium tuberculosis
4.1.3.36 Y287F no activity Mycobacterium tuberculosis
4.1.3.36 Y97F no activity Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.3.36 0.0031
-
 2-succinylbenzoyl-CoA D185N mutant protein, pH 7.0, 25°C Mycobacterium tuberculosis
4.1.3.36 0.0048
-
 2-succinylbenzoyl-CoA D185E mutant protein, pH 7.0, 25°C Mycobacterium tuberculosis
4.1.3.36 0.0224
-
 2-succinylbenzoyl-CoA wild type protein, pH 7.0, 25°C Mycobacterium tuberculosis
4.1.3.36 0.0259
-
 o-succinylbenzoyl-CoA pH 7.0, 25°C Escherichia coli
4.1.3.36 0.0402
-
 2-succinylbenzoyl-CoA S190A mutant protein, pH 7.0, 25°C Mycobacterium tuberculosis
4.1.3.36 0.106
-
 2-(3-carboxypropyl)-benzoyl-CoA wild type protein protein, pH 7.0, 25°C Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.3.36 30000
-
 6 * 30000, SDS-PAGE, crystallization Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.36 Escherichia coli
-
-
-
4.1.3.36 Mycobacterium tuberculosis P9WNP5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.3.36
-
 Mycobacterium tuberculosis
4.1.3.36 His-tag affinity chromatography, tag removed Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.36 2-(3-carboxypropyl)-benzoyl-CoA
-
 Mycobacterium tuberculosis ?
-
 ?
4.1.3.36 2-succinylbenzoyl-CoA
-
 Mycobacterium tuberculosis 1,4-dihydroxy-2-naphthoyl-CoA + H2O
-
 ?
4.1.3.36 o-succinylbenzoyl-CoA
-
 Escherichia coli 1,4-dihydroxy-2-naphthoyl-CoA + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.3.36 hexamer crystallization Mycobacterium tuberculosis
4.1.3.36 hexamer 6 * 30000, SDS-PAGE, crystallization Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
4.1.3.36 1,4-dihydroxy-2-naphthoyl-CoA synthase
-
 Escherichia coli
4.1.3.36 1,4-dihydroxy-2-naphthoyl-CoA synthase
-
 Mycobacterium tuberculosis
4.1.3.36 MenB
-
 Escherichia coli
4.1.3.36 MenB
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.3.36 0.00022
-
 2-succinylbenzoyl-CoA D185N mutant protein, pH 7.0, 25°C Mycobacterium tuberculosis
4.1.3.36 0.00025
-
 2-(3-carboxypropyl)-benzoyl-CoA wild type protein protein, pH 7.0, 25°C Mycobacterium tuberculosis
4.1.3.36 0.0017
-
 2-succinylbenzoyl-CoA S190A mutant protein, pH 7.0, 25°C Mycobacterium tuberculosis
4.1.3.36 0.0023
-
 2-succinylbenzoyl-CoA D185E mutant protein, pH 7.0, 25°C Mycobacterium tuberculosis
4.1.3.36 0.062
-
 o-succinylbenzoyl-CoA pH 7.0, 25°C Escherichia coli
4.1.3.36 0.462
-
 2-succinylbenzoyl-CoA wild type protein, pH 7.0, 25°C Mycobacterium tuberculosis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.1.3.36 0.0024
-
 2-(3-carboxypropyl)-benzoyl-CoA wild type protein protein, pH 7.0, 25°C Mycobacterium tuberculosis
4.1.3.36 0.04
-
 2-succinylbenzoyl-CoA S190A mutant protein, pH 7.0, 25°C Mycobacterium tuberculosis
4.1.3.36 0.07
-
 2-succinylbenzoyl-CoA D185N mutant protein, pH 7.0, 25°C Mycobacterium tuberculosis
4.1.3.36 0.48
-
 2-succinylbenzoyl-CoA D185E mutant protein, pH 7.0, 25°C Mycobacterium tuberculosis
4.1.3.36 2.4
-
 o-succinylbenzoyl-CoA pH 7.0, 25°C Escherichia coli
4.1.3.36 21
-
 2-succinylbenzoyl-CoA wild type protein, pH 7.0, 25°C Mycobacterium tuberculosis