BRENDA - Enzyme Database

Kinetic mechanism for the excision of hypoxanthine by Escherichia coli AlkA and evidence for binding to DNA ends

Zhao, B.; OBrien, P.J.; Biochemistry 50, 4350-4359 (2011) View publication on PubMedView publication on EuropePMC

Data extracted from this reference:

Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
3.2.2.21
expressed in Escherichia coli
Escherichia coli
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.2.21
abasic-containing DNA
potent inhibitor
Escherichia coli
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.2.21
0.042
-
duplex oligonucleotide substrate containing ethenoadenine and hypoxanthine
at 37°C, in 50 mM Na-MES buffer (pH 6.1)
Escherichia coli
Organism
EC Number
Organism
UniProt
Commentary
Textmining
3.2.2.21
Escherichia coli
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
3.2.2.21
-
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3.2.2.21
duplex oligonucleotide substrate containing ethenoadenine and hypoxanthine + H2O
-
714251
Escherichia coli
ethenoadenine + hypoxanthine + oligonucleotide
-
-
-
?
3.2.2.21
additional information
the enzyme binds preferentially DNA ends, more tightly than hypoxanthine lesions, exhibits significant product inhibition under multiple-turnover conditions, and binds approximately 10fold more tightly to an abasic site than to a hypoxanthine lesion site
714251
Escherichia coli
?
-
-
-
?
Synonyms
EC Number
Synonyms
Commentary
Organism
3.2.2.21
3-methyladenine DNA glycosylase II
-
Escherichia coli
3.2.2.21
AlkA
-
Escherichia coli
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.2.2.21
0.00125
-
duplex oligonucleotide substrate containing ethenoadenine and hypoxanthine
at 37°C, in 50 mM Na-MES buffer (pH 6.1)
Escherichia coli
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.2.21
6.1
-
-
Escherichia coli
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.2.21
expressed in Escherichia coli
Escherichia coli
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.2.21
abasic-containing DNA
potent inhibitor
Escherichia coli
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.2.21
0.042
-
duplex oligonucleotide substrate containing ethenoadenine and hypoxanthine
at 37°C, in 50 mM Na-MES buffer (pH 6.1)
Escherichia coli
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.2.21
-
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3.2.2.21
duplex oligonucleotide substrate containing ethenoadenine and hypoxanthine + H2O
-
714251
Escherichia coli
ethenoadenine + hypoxanthine + oligonucleotide
-
-
-
?
3.2.2.21
additional information
the enzyme binds preferentially DNA ends, more tightly than hypoxanthine lesions, exhibits significant product inhibition under multiple-turnover conditions, and binds approximately 10fold more tightly to an abasic site than to a hypoxanthine lesion site
714251
Escherichia coli
?
-
-
-
?
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.2.2.21
0.00125
-
duplex oligonucleotide substrate containing ethenoadenine and hypoxanthine
at 37°C, in 50 mM Na-MES buffer (pH 6.1)
Escherichia coli
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.2.21
6.1
-
-
Escherichia coli
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
3.2.2.21
30
-
duplex oligonucleotide substrate containing ethenoadenine and hypoxanthine
at 37°C, in 50 mM Na-MES buffer (pH 6.1)
Escherichia coli
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
3.2.2.21
30
-
duplex oligonucleotide substrate containing ethenoadenine and hypoxanthine
at 37°C, in 50 mM Na-MES buffer (pH 6.1)
Escherichia coli