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Literature summary extracted from

  • Chakladar, S.; Cheng, L.; Choi, M.; Liu, J.; Bennet, A.J.
    Mechanistic evaluation of MelA alpha-galactosidase from Citrobacter freundii: a family 4 glycosyl hydrolase in which oxidation is rate-limiting (2011), Biochemistry, 50, 4298-4308.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.22 2-mercaptoethanol required for enzymatic activity Citrobacter freundii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.22 Mn2+ required for enzymatic activity Citrobacter freundii

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.22 Citrobacter freundii
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.1.22 raffinose + H2O = sucrose + alpha-D-galactopyranose the recombinant enzyme catalyzes the hydrolysis of phenyl R-galactosides via a redox elimination-addition mechanism involving oxidation of the hydroxyl group at C-3 and elimination of phenol across the C-1-C-2 bond to give an enzyme-bound glycal intermediate Citrobacter freundii
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.22 4-nitrophenyl-alpha-D-galactopyranoside + H2O
-
 Citrobacter freundii 4-nitrophenol + alpha-D-galactopyranose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.22 alpha-galactosidase
-
 Citrobacter freundii
3.2.1.22 MelA
-
 Citrobacter freundii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.22 37
-
 assay at Citrobacter freundii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.22 0.12
-
 4-nitrophenyl-alpha-D-galactopyranoside pH 4.5, 37°C Citrobacter freundii
3.2.1.22 0.23
-
 4-nitrophenyl-alpha-D-galactopyranoside pH 9.0, 37°C Citrobacter freundii
3.2.1.22 0.3
-
 4-nitrophenyl-alpha-D-galactopyranoside pH 6.7, 37°C Citrobacter freundii
3.2.1.22 0.62
-
 4-nitrophenyl-alpha-D-galactopyranoside pH 8.7, 37°C Citrobacter freundii
3.2.1.22 1.05
-
 4-nitrophenyl-alpha-D-galactopyranoside pH 7.1, 37°C Citrobacter freundii
3.2.1.22 1.37
-
 4-nitrophenyl-alpha-D-galactopyranoside pH 8.4, 37°C Citrobacter freundii
3.2.1.22 1.68
-
 4-nitrophenyl-alpha-D-galactopyranoside pH 7.5, 37°C Citrobacter freundii
3.2.1.22 1.75
-
 4-nitrophenyl-alpha-D-galactopyranoside pH 8.0, 37°C Citrobacter freundii
3.2.1.22 2.07
-
 4-nitrophenyl-alpha-D-galactopyranoside pH 7.8, 37°C Citrobacter freundii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.22 7.5
-
 assay at Citrobacter freundii

Cofactor

EC Number Cofactor Comment Organism Structure
3.2.1.22 NAD+ required for enzymatic activity Citrobacter freundii

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.22 0.091
-
 4-nitrophenyl-alpha-D-galactopyranoside pH 4.5, 37°C Citrobacter freundii
3.2.1.22 0.81
-
 4-nitrophenyl-alpha-D-galactopyranoside pH 6.7, 37°C Citrobacter freundii
3.2.1.22 0.96
-
 4-nitrophenyl-alpha-D-galactopyranoside pH 9.0, 37°C Citrobacter freundii
3.2.1.22 2.7
-
 4-nitrophenyl-alpha-D-galactopyranoside pH 7.1, 37°C Citrobacter freundii
3.2.1.22 5
-
 4-nitrophenyl-alpha-D-galactopyranoside pH 8.7, 37°C Citrobacter freundii
3.2.1.22 5.8
-
 4-nitrophenyl-alpha-D-galactopyranoside pH 7.5, 37°C Citrobacter freundii
3.2.1.22 8.9
-
 4-nitrophenyl-alpha-D-galactopyranoside pH 8.0, 37°C Citrobacter freundii
3.2.1.22 9.9
-
 4-nitrophenyl-alpha-D-galactopyranoside pH 7.8, 37°C Citrobacter freundii
3.2.1.22 15.6
-
 4-nitrophenyl-alpha-D-galactopyranoside pH 8.4, 37°C Citrobacter freundii