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Literature summary extracted from

  • Yoshida, S.; Park, D.S.; Bae, B.; Mackie, R.; Cann, I.K.; Nair, S.K.
    Structural and functional analyses of a glycoside hydrolase family 5 enzyme with an unexpected beta-fucosidase activity (2011), Biochemistry, 50, 3369-3375.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.38 expression in Escherichia coli Prevotella bryantii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.38 to 2.2 A resolution, space group C222. The overall topology consists of a central catalytic (alpha/beta)8 barrel composed of residues Thr14 through Phe413 flanked by amino- and carboxy-terminal extensions that consist of sheets that form an independent beta-sandwich domain. This small beta domain associates with the backside of the catalytic domain, and two domains associate through hydrophobic interactions Prevotella bryantii

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.38 E205A complete loss of activity Prevotella bryantii
3.2.1.38 E239A complete loss of activity Prevotella bryantii

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.38 Prevotella bryantii
-
 glycoside hydrolase family 5 enzyme
-
3.2.1.38 Prevotella bryantii B14
-
 glycoside hydrolase family 5 enzyme
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.38 4-nitrophenyl alpha-L-arabinopyranoside + H2O about 60% of the activity with 4-nitrophenyl beta-D-fucopyranoside Prevotella bryantii 4-nitrophenol + alpha-L-arabinopyranose
-
 ?
3.2.1.38 4-nitrophenyl alpha-L-arabinopyranoside + H2O about 60% of the activity with 4-nitrophenyl beta-D-fucopyranoside Prevotella bryantii B14 4-nitrophenol + alpha-L-arabinopyranose
-
 ?
3.2.1.38 4-nitrophenyl beta-D-fucopyranoside + H2O best substrate Prevotella bryantii 4-nitrophenol + beta-D-fucopyranose
-
 ?
3.2.1.38 4-nitrophenyl beta-D-fucopyranoside + H2O best substrate Prevotella bryantii B14 4-nitrophenol + beta-D-fucopyranose
-
 ?
3.2.1.38 4-nitrophenyl beta-D-galactopyranoside + H2O about 30% of the activity with 4-nitrophenyl beta-D-fucopyranoside Prevotella bryantii 4-nitrophenol + beta-D-galactopyranose
-
 ?
3.2.1.38 4-nitrophenyl beta-D-galactopyranoside + H2O about 30% of the activity with 4-nitrophenyl beta-D-fucopyranoside Prevotella bryantii B14 4-nitrophenol + beta-D-galactopyranose
-
 ?
3.2.1.38 additional information no substrate: xylan polysaccharides Prevotella bryantii ?
-
 ?
3.2.1.38 additional information no substrate: xylan polysaccharides Prevotella bryantii B14 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.38 FucA
-
 Prevotella bryantii
3.2.1.38 Pb280
-
 Prevotella bryantii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.38 5
-
 protein precipitates below Prevotella bryantii

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.38 5
-
 protein precipitates below Prevotella bryantii