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Literature summary extracted from

  • Briggs, G.D.; Gordon, S.L.; Dickson, P.W.
    Mutational analysis of catecholamine binding in tyrosine hydroxylase (2011), Biochemistry, 50, 1545-1555.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.16.2 expressed in Escherichia coli BL21(DE3) Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.14.16.2 A297L outer edge of the active site Homo sapiens
1.14.16.2 E332D active site residue, 10fold reduction in activity, close to the catalytic iron Homo sapiens
1.14.16.2 E332Q active site residue, no activity, close to the catalytic iron Homo sapiens
1.14.16.2 F300A active site residue Homo sapiens
1.14.16.2 F300Y active site residue Homo sapiens
1.14.16.2 L294A protrudes into catalytic cleft Homo sapiens
1.14.16.2 L294Y protrudes into catalytic cleft Homo sapiens
1.14.16.2 Y371F active site residue, close to the catalytic iron Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.16.2 dopamine
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.16.2 0.0056
-
 tetrahydrobiopterin wild type, phosphorylated protein, pH 7.2, 30°C Homo sapiens
1.14.16.2 0.0068
-
 tetrahydrobiopterin wild type protein, dopamine bound but removed from low-affinity site, pH 7.2, 30°C Homo sapiens
1.14.16.2 0.0072
-
 tetrahydrobiopterin E332D phosphorylated mutant protein, pH 7.2, 30°C Homo sapiens
1.14.16.2 0.0083
-
 tetrahydrobiopterin E332D mutant protein, pH 7.2, 30°C Homo sapiens
1.14.16.2 0.0099
-
 tetrahydrobiopterin L294Y phosphorylated mutant protein, pH 7.2, 30°C Homo sapiens
1.14.16.2 0.012
-
 tetrahydrobiopterin F300Y phosphorylated mutant protein, pH 7.2, 30°C Homo sapiens
1.14.16.2 0.014
-
 tetrahydrobiopterin L294A mutant protein, pH 7.2, 30°C Homo sapiens
1.14.16.2 0.014
-
 tetrahydrobiopterin wild type protein, pH 7.2, 30°C Homo sapiens
1.14.16.2 0.015
-
 tetrahydrobiopterin L294Y mutant protein, pH 7.2, 30°C Homo sapiens
1.14.16.2 0.017
-
 tetrahydrobiopterin A297L phosphorylated mutant protein, pH 7.2, 30°C Homo sapiens
1.14.16.2 0.017
-
 tetrahydrobiopterin Y371F mutant protein, pH 7.2, 30°C Homo sapiens
1.14.16.2 0.02
-
 tetrahydrobiopterin A297L mutant protein, pH 7.2, 30°C Homo sapiens
1.14.16.2 0.026
-
 tetrahydrobiopterin F300Y mutant protein, pH 7.2, 30°C Homo sapiens
1.14.16.2 0.027
-
 tetrahydrobiopterin A297L mutant protein, dopamine bound to the high-affinity site, pH 7.2, 30°C Homo sapiens
1.14.16.2 0.027
-
 tetrahydrobiopterin Y371F mutant protein, dopamine bound to the high-affinity site, pH 7.2, 30°C Homo sapiens
1.14.16.2 0.029
-
 tetrahydrobiopterin L294A phosphorylated mutant protein, pH 7.2, 30°C Homo sapiens
1.14.16.2 0.029
-
 tetrahydrobiopterin Y371F phosphorylated mutant protein, pH 7.2, 30°C Homo sapiens
1.14.16.2 0.034
-
 tetrahydrobiopterin E332D mutant protein, dopamine bound to the high-affinity site, pH 7.2, 30°C Homo sapiens
1.14.16.2 0.054
-
 tetrahydrobiopterin F300A mutant protein, pH 7.2, 30°C Homo sapiens
1.14.16.2 0.07
-
 tetrahydrobiopterin F300A phosphorylated mutant protein, pH 7.2, 30°C Homo sapiens
1.14.16.2 0.134
-
 tetrahydrobiopterin wild type protein, dopamine bound to the high-affinity site, pH 7.2, 30°C Homo sapiens
1.14.16.2 0.356
-
 tetrahydrobiopterin wild type protein, in the presence of dopamine, pH 7.2, 30°C Homo sapiens
1.14.16.2 0.507
-
 tetrahydrobiopterin wild type, phosphorylated protein, in the presence of dopamine, pH 7.2, 30°C Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.16.2 Fe2+
-
 Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.14.16.2 Homo sapiens P07101
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.16.2 heparin Sepharose chromatography Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.16.2 L-tyrosine + tetrahydrobiopterin + O2
-
 Homo sapiens 3,4-dihydroxy-L-phenylalanine + 4a-hydroxytetrahydrobiopterin + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.16.2 tyrosine hydroxylase
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.16.2 0.017
-
 tetrahydrobiopterin wild type protein, in the presence of 0.01 mM dopamine, pH 7.2, 30°C Homo sapiens
1.14.16.2 0.047
-
 tetrahydrobiopterin E332D phosphorylated mutant protein, pH 7.2, 30°C Homo sapiens
1.14.16.2 0.06
-
 tetrahydrobiopterin E332D mutant protein, pH 7.2, 30°C Homo sapiens
1.14.16.2 0.068
-
 tetrahydrobiopterin L294Y phosphorylated mutant protein, pH 7.2, 30°C Homo sapiens
1.14.16.2 0.088
-
 tetrahydrobiopterin F300A mutant protein, pH 7.2, 30°C Homo sapiens
1.14.16.2 0.11
-
 tetrahydrobiopterin F300A phosphorylated mutant protein, pH 7.2, 30°C Homo sapiens
1.14.16.2 0.12
-
 tetrahydrobiopterin L294Y mutant protein, pH 7.2, 30°C Homo sapiens
1.14.16.2 0.14
-
 tetrahydrobiopterin L294A phosphorylated mutant protein, pH 7.2, 30°C Homo sapiens
1.14.16.2 0.18
-
 tetrahydrobiopterin L294A mutant protein, pH 7.2, 30°C Homo sapiens
1.14.16.2 0.2
-
 tetrahydrobiopterin Y371F mutant protein, pH 7.2, 30°C Homo sapiens
1.14.16.2 0.22
-
 tetrahydrobiopterin A297L phosphorylated mutant protein, pH 7.2, 30°C Homo sapiens
1.14.16.2 0.27
-
 tetrahydrobiopterin A297L mutant protein, pH 7.2, 30°C Homo sapiens
1.14.16.2 0.27
-
 tetrahydrobiopterin F300Y phosphorylated mutant protein, pH 7.2, 30°C Homo sapiens
1.14.16.2 0.3
-
 tetrahydrobiopterin F300Y mutant protein, pH 7.2, 30°C Homo sapiens
1.14.16.2 0.3
-
 tetrahydrobiopterin Y371F phosphorylated mutant protein, pH 7.2, 30°C Homo sapiens
1.14.16.2 0.47
-
 tetrahydrobiopterin wild type, phosphorylated protein, pH 7.2, 30°C Homo sapiens
1.14.16.2 0.58
-
 tetrahydrobiopterin wild type protein, pH 7.2, 30°C Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.14.16.2 0.000256
-
 wild type, phosphorylated protein, pH 7.2, 30°C Homo sapiens dopamine
1.14.16.2 0.0004
-
 wild type protein, pH 7.2, 30°C Homo sapiens dopamine
1.14.16.2 0.00093
-
 F300Y mutant protein, pH 7.2, 30°C Homo sapiens dopamine
1.14.16.2 0.00098
-
 Y371F mutant protein, pH 7.2, 30°C Homo sapiens dopamine
1.14.16.2 0.00107
-
 L294A mutant protein, pH 7.2, 30°C Homo sapiens dopamine
1.14.16.2 0.00143
-
 E332D mutant protein, pH 7.2, 30°C Homo sapiens dopamine
1.14.16.2 0.00248
-
 E332D phosphorylated mutant protein, pH 7.2, 30°C Homo sapiens dopamine
1.14.16.2 0.0136
-
 E332D phosphorylated mutant protein, pH 7.2, 30°C Homo sapiens dopamine

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.14.16.2 1.7
-
 tetrahydrobiopterin F300A mutant protein, pH 7.2, 30°C Homo sapiens
1.14.16.2 1.7
-
 tetrahydrobiopterin F300A phosphorylated mutant protein, pH 7.2, 30°C Homo sapiens
1.14.16.2 5
-
 tetrahydrobiopterin L294A phosphorylated mutant protein, pH 7.2, 30°C Homo sapiens
1.14.16.2 6.5
-
 tetrahydrobiopterin E332D phosphorylated mutant protein, pH 7.2, 30°C Homo sapiens
1.14.16.2 7
-
 tetrahydrobiopterin L294Y phosphorylated mutant protein, pH 7.2, 30°C Homo sapiens
1.14.16.2 7.3
-
 tetrahydrobiopterin E332D mutant protein, pH 7.2, 30°C Homo sapiens
1.14.16.2 7.7
-
 tetrahydrobiopterin L294Y mutant protein, pH 7.2, 30°C Homo sapiens
1.14.16.2 10.7
-
 tetrahydrobiopterin Y371F phosphorylated mutant protein, pH 7.2, 30°C Homo sapiens
1.14.16.2 11.2
-
 tetrahydrobiopterin F300Y mutant protein, pH 7.2, 30°C Homo sapiens
1.14.16.2 11.7
-
 tetrahydrobiopterin Y371F mutant protein, pH 7.2, 30°C Homo sapiens
1.14.16.2 12.5
-
 tetrahydrobiopterin A297L phosphorylated mutant protein, pH 7.2, 30°C Homo sapiens
1.14.16.2 13
-
 tetrahydrobiopterin L294A mutant protein, pH 7.2, 30°C Homo sapiens
1.14.16.2 13.7
-
 tetrahydrobiopterin A297L mutant protein, pH 7.2, 30°C Homo sapiens
1.14.16.2 21.8
-
 tetrahydrobiopterin F300Y phosphorylated mutant protein, pH 7.2, 30°C Homo sapiens
1.14.16.2 45.7
-
 tetrahydrobiopterin wild type protein, pH 7.2, 30°C Homo sapiens
1.14.16.2 89.3
-
 tetrahydrobiopterin wild type, phosphorylated protein, pH 7.2, 30°C Homo sapiens