Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Nathania, L.; Nicholson, A.W.
    Thermotoga maritima ribonuclease III. Characterization of thermostable biochemical behavior and analysis of conserved base pairs that function as reactivity epitopes for the Thermotoga 23S rRNA precursor (2010), Biochemistry, 49, 7164-7178.
    View publication on PubMedView publication on EuropePMC

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.26.3 Co2+ less effective than Mg2+ Thermotoga maritima
3.1.26.3 Mg2+ activates optimally at 1 mM Thermotoga maritima
3.1.26.3 Mn2+ less effective than Mg2+ Thermotoga maritima
3.1.26.3 additional information the enzyme functions optimally at pH 8 and about 50-80 mM salt concentrations Thermotoga maritima
3.1.26.3 Ni2+ less effective than Mg2+ Thermotoga maritima

Organism

EC Number Organism UniProt Comment Textmining
3.1.26.3 Thermotoga maritima
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.26.3 additional information substrate specificity with RNA duplex substrates, overview. The proximal box is a primary reactivity epitope for Tm-23S[hp] RNA. a CG or GC bp substitution at pb position 2 reduces the relative reactivity to 0.1 and 0.3, respectively, while a CG or GC substitution at pb position 4 provides a relative reactivity of 0.1 or 0.4, respectively. At pb position 3, only the AU bp substitution causes a significant drop in relative reactivity, while none of the bp substitutions at pb position 1 has a significant effect Thermotoga maritima ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.26.3 ribonuclease III
-
 Thermotoga maritima
3.1.26.3 RNase III
-
 Thermotoga maritima

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.26.3 40 70 RNase III catalytic activity exhibits a broad optimal temperature range Thermotoga maritima

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.26.3 40 95 activity range Thermotoga maritima

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.26.3 8
-
 assay at Thermotoga maritima

General Information

EC Number General Information Comment Organism
3.1.26.3 evolution Tm-RNase III cleaves an Ec-RNase III substrate with identical specificity and is inhibited by antideterminant bp that also inhibit Ec-RNase III, indicating the conservation, across a broad phylogenetic distance, of positive and negative determinants of reactivity of bacterial RNase III substrates Thermotoga maritima
3.1.26.3 physiological function cleavage of double-stranded RNA by ribonuclease III is a conserved early step in bacterial rRNA maturation, mechanism of dsRNA cleavage by RNase III, overview Thermotoga maritima