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Literature summary extracted from

  • Sharma, N.K.; Pan, J.J.; Poulter, C.D.
    Type II isopentenyl diphosphate isomerase: probing the mechanism with alkyne/allene diphosphate substrate analogues (2010), Biochemistry, 49, 6228-6233.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.3.2 expressed in Escherichia coli Thermus thermophilus
5.3.3.2 expressed in Escherichia coli Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.3.3.2 2,3-butadien-1-yl diphosphate competitive inhibitor Escherichia coli
5.3.3.2 2,3-butadien-1-yl diphosphate competitive inhibitor Thermus thermophilus
5.3.3.2 3-butyn-1-yl diphosphate competitive inhibitor Escherichia coli
5.3.3.2 3-butyn-1-yl diphosphate competitive inhibitor Thermus thermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.3.3.2 Mg2+ required for activity Thermus thermophilus
5.3.3.2 Mg2+ required for activity Escherichia coli
5.3.3.2 Zn2+ IDI-1 is a zinc-metalloprotein Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
5.3.3.2 Escherichia coli
-
-
-
5.3.3.2 Thermus thermophilus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.3.2 isopentenyl diphosphate
-
 Thermus thermophilus dimethylallyl diphosphate
-
 r
5.3.3.2 isopentenyl diphosphate
-
 Escherichia coli dimethylallyl diphosphate
-
 r
5.3.3.2 additional information 3-butyn-1-yl diphosphate and 2,3-butadien-1-yl diphosphate are no substrates for IDI-1 Escherichia coli ?
-
 ?
5.3.3.2 additional information 3-butyn-1-yl diphosphate and 2,3-butadien-1-yl diphosphate are no substrates for IDI-2 Thermus thermophilus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.3.3.2 IDI-1
-
 Escherichia coli
5.3.3.2 Idi-2
-
 Thermus thermophilus
5.3.3.2 type I isopentenyl diphosphate isomerase
-
 Escherichia coli
5.3.3.2 type II isopentenyl diphosphate isomerase
-
 Thermus thermophilus

Cofactor

EC Number Cofactor Comment Organism Structure
5.3.3.2 FMN IDI-2 requires reduced flavin Thermus thermophilus
5.3.3.2 NADPH
-
 Thermus thermophilus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
5.3.3.2 0.031
-
 2,3-butadien-1-yl diphosphate in 50 mM HEPES (pH 7.2) containing 10 mM MgCl2, 200 mM KCl, 1 mg/ml bovine serum albumin, 0.5 mM dithiothreitol, at 37°C Escherichia coli
5.3.3.2 0.036
-
 2,3-butadien-1-yl diphosphate in 200 mM HEPES (pH 7.0) containing 2 mM MgCl2, 0.04 mM FMN, 2 mM NADPH, at 37°C Thermus thermophilus
5.3.3.2 0.048
-
 3-butyn-1-yl diphosphate in 200 mM HEPES (pH 7.0) containing 2 mM MgCl2, 0.04 mM FMN, 2 mM NADPH, at 37°C Thermus thermophilus
5.3.3.2 0.049
-
 3-butyn-1-yl diphosphate in 50 mM HEPES (pH 7.2) containing 10 mM MgCl2, 200 mM KCl, 1 mg/ml bovine serum albumin, 0.5 mM dithiothreitol, at 37°C Escherichia coli