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Literature summary extracted from

  • Jiang, W.; Xie, J.; Varano, P.T.; Krebs, C.; Bollinger, J.M.
    Two distinct mechanisms of inactivation of the class Ic ribonucleotide reductase from Chlamydia trachomatis by hydroxyurea: implications for the protein gating of intersubunit electron transfer (2010), Biochemistry, 49, 5340-5349.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.17.4.1 Hydroxyurea inactivates both Ia/b and Ic beta2 subunits by reducing their C oxidants, reacts with the MnIV/FeIII cofactor to give two distinct products: the homogeneous MnIII/FeIII-beta2 complex, which forms only under turnover conditions, in the presence of alpha2 and the substrate, and a distinct, diamagnetic Mn/Fe cluster, which forms about 900fold less rapidly as a second phase in the reaction under turnover conditions and as the sole outcome in the reaction of MnIV/FeIII-beta2 only Chlamydia trachomatis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.17.4.1 Fe3+ MnIV/FeIII cofactor Chlamydia trachomatis
1.17.4.1 Mn(IV) MnIV/FeIII cofactor Chlamydia trachomatis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.4.1 additional information Chlamydia trachomatis catalysis by a class I RNR begins when a cysteine residue in the alpha2 subunit is oxidized to a thiyl radical by a cofactor about 35 A away in the beta2 subunit. In a class Ia or Ib RNR, a stable tyrosyl radical is the C oxidant, whereas a MnIV/FeIII cluster serves this function in the class Ic enzyme from Chlamydia trachomatis ?
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 ?

Organism

EC Number Organism UniProt Comment Textmining
1.17.4.1 Chlamydia trachomatis
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.4.1 additional information catalysis by a class I RNR begins when a cysteine residue in the alpha2 subunit is oxidized to a thiyl radical by a cofactor about 35 A away in the beta2 subunit. In a class Ia or Ib RNR, a stable tyrosyl radical is the C oxidant, whereas a MnIV/FeIII cluster serves this function in the class Ic enzyme from Chlamydia trachomatis Chlamydia trachomatis ?
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 ?

Subunits

EC Number Subunits Comment Organism
1.17.4.1 tetramer alpha2beta2, with subunits alpha1, alpha2, beta1, and beta2 Chlamydia trachomatis

Synonyms

EC Number Synonyms Comment Organism
1.17.4.1 class I RNR
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 Chlamydia trachomatis
1.17.4.1 class Ic ribonucleotide reductase
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 Chlamydia trachomatis

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.4.1 MnIV/FeIII cofactor electron transfer mechanism and conformational identification, role in reaction and mechanism, detailed overview Chlamydia trachomatis