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Literature summary extracted from

  • Ukaegbu, U.E.; Kantz, A.; Beaton, M.; Gassner, G.T.; Rosenzweig, A.C.
    Structure and ligand binding properties of the epoxidase component of styrene monooxygenase (2010), Biochemistry, 49, 1678-1688.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.14.11 the crystal structure of the N-terminally histidine-tagged epoxidase component of this system, NSMOA, determined to 2.3 A resolution, indicates the enzyme exists as a homodimer in which each monomer forms two distinct domains Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.11 styrene + FADH2 + O2 Pseudomonas putida
-
 (S)-2-phenyloxirane + FAD + H2O
-
 ?
1.14.14.11 styrene + FADH2 + O2 Pseudomonas putida S12
-
 (S)-2-phenyloxirane + FAD + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.11 Pseudomonas putida O33471
-
-
1.14.14.11 Pseudomonas putida S12 O33471
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.14.11 N-terminally histidine-tagged styrene monooxygenase Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.11 styrene + FADH2 + O2
-
 Pseudomonas putida (S)-2-phenyloxirane + FAD + H2O
-
 ?
1.14.14.11 styrene + FADH2 + O2 preferred reaction order in which flavin reduction and reaction with oxygen precede the binding of styrene Pseudomonas putida (S)-2-phenyloxirane + FAD + H2O
-
 ?
1.14.14.11 styrene + FADH2 + O2
-
 Pseudomonas putida S12 (S)-2-phenyloxirane + FAD + H2O
-
 ?
1.14.14.11 styrene + FADH2 + O2 preferred reaction order in which flavin reduction and reaction with oxygen precede the binding of styrene Pseudomonas putida S12 (S)-2-phenyloxirane + FAD + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.14.11 homodimer each monomer forms two distinct domains Pseudomonas putida

Synonyms

EC Number Synonyms Comment Organism
1.14.14.11 NSMOA
-
 Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.11 FADH2 flavin binding and redox equilibria are tightly coupled such that reduced FAD binds apo NSMOA about 8000times more tightly than the oxidized coenzyme Pseudomonas putida