Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Cotruvo, J.A.; Stubbe, J.
    An active dimanganese(III)-tyrosyl radical cofactor in Escherichia coli class Ib ribonucleotide reductase (2010), Biochemistry, 49, 1297-1309.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.17.4.1 dATP
-
 Escherichia coli
1.17.4.1 NrdI is an essential player in Escherichia coli class Ib RNR cluster assembly, overview. Preparation of recombinant N-terminally His6-tagged NrdI Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.17.4.1 Mg2+ activates Escherichia coli
1.17.4.1 Mn3+ the MnIII2-tyrosyl radical cofactor, not the diferric-tyrosyl radical one, is the active metallocofactor in vivo Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.17.4.1 Escherichia coli
-
-
-

Subunits

EC Number Subunits Comment Organism
1.17.4.1 tetramer class Ib RNR is composed of two homodimeric subunits: alpha2 or NrdE, where nucleotide reduction occurs, and beta2 or NrdF, which contains an unidentified metallocofactor that initiates nucleotide reduction Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
1.17.4.1 class Ib ribonucleotide reductase
-
 Escherichia coli
1.17.4.1 class Ib RNR
-
 Escherichia coli
1.17.4.1 NrdE
-
 Escherichia coli
1.17.4.1 NrdF
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.17.4.1 37
-
 assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.17.4.1 7.6
-
 assay at Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.4.1 dimanganese(III)-tyrosyl radical cofactor the dimanganese(III)-tyrosyl radical cofactor, not the diferric-tyrosyl radical one, is the active metallocofactor in vivo Escherichia coli
1.17.4.1 additional information analysis of composition and conformation, and of the reaction mechanism of the metallo-cofactor, detailed overview Escherichia coli

Expression

EC Number Organism Comment Expression
1.17.4.1 Escherichia coli class Ib RNR is expressed under iron-limited and oxidative stress conditions up

General Information

EC Number General Information Comment Organism
1.17.4.1 physiological function Escherichia coli class Ib RNR converts nucleoside 5'-diphosphates to deoxynucleoside 5'-diphosphates and is expressed under iron-limited and oxidative stress conditions Escherichia coli