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Literature summary extracted from

  • Brito, J.A.; Sousa, F.L.; Stelter, M.; Bandeiras, T.M.; Vonrhein, C.; Teixeira, M.; Pereira, M.M.; Archer, M.
    Structural and functional insights into sulfide:quinone oxidoreductase (2009), Biochemistry, 48, 5613-5622.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.8.5.4 hanging drop vapor diffusion method, using 2.2 M NH4H2PO4 and 100 mM Tris-HCl, pH 8.5 (final pH of the solution is 4.5), or 2.2 M NH4H2PO4/K2HPO4 at pH 4.5 Acidianus ambivalens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.5.4 cyanide
-
 Acidianus ambivalens
1.8.5.4 HQNO a quinone competitive inhibitor Acidianus ambivalens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.8.5.4 membrane
-
 Acidianus ambivalens 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.5.4 48000
-
 about 48000 Da, SDS-PAGE Acidianus ambivalens

Organism

EC Number Organism UniProt Comment Textmining
1.8.5.4 Acidianus ambivalens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.5.4
-
 Acidianus ambivalens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.5.4 0.127
-
 using 2,3-dimethyl-1,4-naphthoquinone as cosubstrate, at 50°C, pH 6.5 Acidianus ambivalens
1.8.5.4 0.194
-
 using menadione as cosubstrate, at 50°C, pH 6.5 Acidianus ambivalens
1.8.5.4 0.47
-
 using decylubiquinone as cosubstrate, at 50°C, pH 6.5 Acidianus ambivalens
1.8.5.4 0.531
-
 using caldariella quinone as cosubstrate, at 50°C, pH 6.5 Acidianus ambivalens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.5.4 sulfide + 2,3-dimethyl-1,4-naphthoquinone
-
 Acidianus ambivalens sulfur + 2,3-dimethyl-1,4-naphthoquinol
-
 ?
1.8.5.4 sulfide + caldariellaquinone
-
 Acidianus ambivalens sulfur + caldariellaquinol
-
 ?
1.8.5.4 sulfide + decylubiquinone
-
 Acidianus ambivalens sulfur + decylubiquinol
-
 ?
1.8.5.4 sulfide + menadione
-
 Acidianus ambivalens sulfur + menadiol
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.5.4 dimer x-ray crystallography Acidianus ambivalens

Synonyms

EC Number Synonyms Comment Organism
1.8.5.4 SQR
-
 Acidianus ambivalens
1.8.5.4 sulfide:quinone oxidoreductase
-
 Acidianus ambivalens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8.5.4 0.1
-
 2,3-dimethyl-1,4-naphthoquinone at 50°C, pH 6.5 Acidianus ambivalens
1.8.5.4 0.15
-
 menadione at 50°C, pH 6.5 Acidianus ambivalens
1.8.5.4 0.38
-
 decylubiquinone at 50°C, pH 6.5 Acidianus ambivalens
1.8.5.4 0.43
-
 caldariella quinone at 50°C, pH 6.5 Acidianus ambivalens

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.5.4 FAD covalently bound Acidianus ambivalens