EC Number | Cloned (Comment) | Organism |
---|---|---|
2.6.1.B6 | - |
Streptomyces venezuelae |
2.6.1.33 | - |
Streptomyces venezuelae |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.6.1.B6 | hanging drop method of vapor diffusion. The structure of DesI is solved in complex with its product, dTDP-4-amino-4,6-dideoxyglucose, to a nominal resolution of 2.1 A. Each subunit of the dimeric enzyme contains 12 alpha-helices and 14 alpha-strands. Crystals belong to the space group P2(1)2(1)2(1) with unit cell dimensions of 59.5 A, 66.8 A, 242.0 A and one dimer per asymmetric unit. Crystal dimensions are typically 1 mm * 0.6 mm * 0.2 mm | Streptomyces venezuelae |
2.6.1.33 | hanging drop method of vapor diffusion. The structure of DesI is solved in complex with its product, dTDP-4-amino-4,6-dideoxyglucose, to a nominal resolution of 2.1 A. Each subunit of the dimeric enzyme contains 12 alpha-helices and 14 alpha-strands. Crystals belong to the space group P2(1)2(1)2(1) with unit cell dimensions of 59.5 A, 66.8 A, 242.0 A and one dimer per asymmetric unit. Crystal dimensions are typically 1 mm * 0.6 mm * 0.2 mm | Streptomyces venezuelae |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.6.1.B6 | dTDP-4-dehydro-6-deoxy-alpha-D-glucopyranose + L-glutamate | Streptomyces venezuelae | the enzyme is involved in biosynthesis of desosamine | dTDP-4-amino-4,6-dideoxy-alpha-D-glucopyranose + 2-oxoglutarate | - |
? | |
2.6.1.33 | dTDP-4-dehydro-6-deoxy-alpha-D-glucopyranose + L-glutamate | Streptomyces venezuelae | the enzyme is involved in biosynthesis of desosamine | dTDP-4-amino-4,6-dideoxy-alpha-D-glucopyranose + 2-oxoglutarate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.6.1.B6 | Streptomyces venezuelae | Q9ZGH0 | - |
- |
2.6.1.33 | Streptomyces venezuelae | Q9ZGH0 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.6.1.B6 | - |
Streptomyces venezuelae |
2.6.1.33 | - |
Streptomyces venezuelae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.6.1.B6 | dTDP-4-dehydro-6-deoxy-alpha-D-glucopyranose + L-glutamate | the enzyme is involved in biosynthesis of desosamine | Streptomyces venezuelae | dTDP-4-amino-4,6-dideoxy-alpha-D-glucopyranose + 2-oxoglutarate | - |
? | |
2.6.1.B6 | dTDP-4-dehydro-6-deoxy-alpha-D-glucopyranose + L-glutamate | the two active sites of the enzyme are located in clefts at the subunit/subunit interface. Electron density corresponding to the bound product demonstrates a covalent bond between the amino group of the product and C-4' of the pyridoxal 5'-phosphate cofactor | Streptomyces venezuelae | dTDP-4-amino-4,6-dideoxy-alpha-D-glucopyranose + 2-oxoglutarate | - |
? | |
2.6.1.33 | dTDP-4-dehydro-6-deoxy-alpha-D-glucopyranose + L-glutamate | the enzyme is involved in biosynthesis of desosamine | Streptomyces venezuelae | dTDP-4-amino-4,6-dideoxy-alpha-D-glucopyranose + 2-oxoglutarate | - |
? | |
2.6.1.33 | dTDP-4-dehydro-6-deoxy-alpha-D-glucopyranose + L-glutamate | the two active sites of the enzyme are located in clefts at the subunit/subunit interface. Electron density corresponding to the bound product demonstrates a covalent bond between the amino group of the product and C-4' of the pyridoxal 5'-phosphate cofactor | Streptomyces venezuelae | dTDP-4-amino-4,6-dideoxy-alpha-D-glucopyranose + 2-oxoglutarate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.6.1.B6 | dimer | each subunit of the dimeric enzyme contains 12 alpha-helices and 14 alpha-strands | Streptomyces venezuelae |
2.6.1.33 | dimer | each subunit of the dimeric enzyme contains 12 alpha-helices and 14 alpha-strands | Streptomyces venezuelae |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.6.1.B6 | DesI | - |
Streptomyces venezuelae |
2.6.1.33 | DesI | - |
Streptomyces venezuelae |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.6.1.B6 | 37 | - |
assay at | Streptomyces venezuelae |
2.6.1.33 | 37 | - |
assay at | Streptomyces venezuelae |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.6.1.B6 | 7.5 | - |
assay at | Streptomyces venezuelae |
2.6.1.33 | 7.5 | - |
assay at | Streptomyces venezuelae |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.6.1.B6 | pyridoxal 5'-phosphate | a pyridoxal-phosphate protein | Streptomyces venezuelae | |
2.6.1.33 | pyridoxal 5'-phosphate | a pyridoxal-phosphate protein | Streptomyces venezuelae |