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Literature summary extracted from

  • Quentmeier, A.; Hellwig, P.; Bardischewsky, F.; Wichmann, R.; Friedrich, C.
    Sulfide dehydrogenase activity of the monomeric flavoprotein SoxF of Paracoccus pantotrophus (2004), Biochemistry, 43, 14696-14703.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.2.3 cyanide 1 mM cyanide inhibits SoxF activity at pH 6.0 by 25% and at pH 8.0 by 92% Paracoccus pantotrophus
1.8.2.3 reduced heart cytochrome c competitive product inhibition Paracoccus pantotrophus
1.8.2.3 sulfite preincubation of SoxF with 1 mM sodium sulfite for 20 min inactivates SoxF (9.0% residual sulfide dehydrogenase activity) Paracoccus pantotrophus
1.8.2.3 sulfur
-
 Paracoccus pantotrophus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.2.3 0.0023
-
 Sulfide at pH 6.0 and 30°C Paracoccus pantotrophus
1.8.2.3 0.116
-
 oxidized heart cytochrome c at pH 6.0 and 30°C Paracoccus pantotrophus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.2.3 42010
-
 calculated from amino acid sequence Paracoccus pantotrophus
1.8.2.3 42797
-
 1 * 42797, electrospray mass spectrometry Paracoccus pantotrophus
1.8.2.3 42832
-
 1 * 42832, electrospray mass spectrometry of SoxF as isolated yields masses of 42797 Da and 42832 Da Paracoccus pantotrophus

Organism

EC Number Organism UniProt Comment Textmining
1.8.2.3 Paracoccus pantotrophus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.2.3 ammonium sulfate precipitation and Q-Sepharose column chromatography Paracoccus pantotrophus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.2.3 additional information SoxF does not catalyze thiosulfate- or sulfite-dependent cytochrome c reduction Paracoccus pantotrophus ?
-
 ?
1.8.2.3 sulfide + oxidized heart cytochrome c
-
 Paracoccus pantotrophus sulfur + reduced heart cytochrome c + H+ sulfur or polysulfide as product ?

Subunits

EC Number Subunits Comment Organism
1.8.2.3 monomer 1 * 42797, electrospray mass spectrometry Paracoccus pantotrophus
1.8.2.3 monomer 1 * 42832, electrospray mass spectrometry of SoxF as isolated yields masses of 42797 Da and 42832 Da Paracoccus pantotrophus

Synonyms

EC Number Synonyms Comment Organism
1.8.2.3 FCSD
-
 Paracoccus pantotrophus
1.8.2.3 flavocytochrome c-sulfide dehydrogenase
-
 Paracoccus pantotrophus
1.8.2.3 SoxF
-
 Paracoccus pantotrophus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8.2.3 3.9
-
 oxidized heart cytochrome c at pH 6.0 and 30°C Paracoccus pantotrophus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.2.3 6
-
-
 Paracoccus pantotrophus

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.2.3 FAD
-
 Paracoccus pantotrophus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.8.2.3 0.0013
-
 sulfur at pH 6.0 and 30°C Paracoccus pantotrophus
1.8.2.3 0.0072
-
 reduced heart cytochrome c at pH 6.0 and 30°C Paracoccus pantotrophus