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Literature summary extracted from

  • Chouchane, S.; Lippai, I.; Magliozzo, R.S.
    Catalase-peroxidase (Mycobacterium tuberculosis KatG) catalysis and isoniazid activation (2000), Biochemistry, 39, 9975-9983.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.11.1.21 medicine KatG is responsible for activation of the antituberculosis antibiotic isoniazid (isonicotinic acid hydrazide) Mycobacterium tuberculosis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.21 expressed in Escherichia coli strain UM262 Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.21 81000
-
 2 * 81000, SDS-PAGE Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.21 Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.21
-
 Mycobacterium tuberculosis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.11.1.21 0.95
-
 peroxidase activity, in 50 mM sodium acetate buffer, pH 5.5, at 25°C Mycobacterium tuberculosis
1.11.1.21 4500
-
 catalase activity, in phosphate buffer, pH 7.5, at 25°C Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.21 H2O2
-
 Mycobacterium tuberculosis O2 + H2O
-
 ?
1.11.1.21 o-dianisidine + H2O2
-
 Mycobacterium tuberculosis oxidized o-dianisidine + H2O
-
 ?
1.11.1.21 tert-butyl hydroperoxide + H2O2
-
 Mycobacterium tuberculosis oxidized tert-butyl hydroperoxide + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.21 homodimer 2 * 81000, SDS-PAGE Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
1.11.1.21 KatG
-
 Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.1.21 heme KatG contains 1 equivalent of heme per dimer Mycobacterium tuberculosis