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Literature summary extracted from

  • Jortzik, E.; Mailu, B.M.; Preuss, J.; Fischer, M.; Bode, L.; Rahlfs, S.; Becker, K.
    Glucose-6-phosphate dehydrogenase-6-phosphogluconolactonase: a unique bifunctional enzyme from Plasmodium falciparum (2011), Biochem. J., 436, 641-650.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.31 cloning of the gene encoding PfGluPho from chromosome 14 of the 3D7 gametocyte cDNA library, heterologous overexpression of PfGluPho using vector pQE30 in Escherichia coli strain M15 Plasmodium falciparum
3.1.1.31 gene h6PGL, overexpression as N-terminally His-tagged enzyme in Escherichia coli strain BL21 using vector pRAREII Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.31 glutathione PfGluPho is inhibited by S-glutathionylation Plasmodium falciparum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.31 additional information
-
additional information detailed kinetic analysis of the bifunctional glucose-6-phosphate dehydrogenase/6-phosphogluconolactonase indicates that PfGluPho functions on the basis of a rapid equilibrium random Bi Bi mechanism, where the binding of the second substrate depends on the first substrate Plasmodium falciparum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.31 6-phospho-D-glucono-1,5-lactone + H2O Plasmodium falciparum
-
6-phospho-D-gluconate
-
?
3.1.1.31 6-phospho-D-glucono-1,5-lactone + H2O Homo sapiens
-
6-phospho-D-gluconate
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.31 Homo sapiens O95336
-
-
3.1.1.31 Plasmodium falciparum
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.1.31 6-phospho-D-glucono-1,5-lactone + H2O = 6-phospho-D-gluconate PfGluPho functions on the basis of a rapid equilibrium random Bi Bi mechanism, where the binding of the second substrate depends on the first substrate Plasmodium falciparum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.31 46
-
recombinant PfGluPho, substrate is 6-phosphoglucono-gamma-lactone, pH 7.0, 25°C Plasmodium falciparum
3.1.1.31 60
-
recombinant PfGluPho, substrate is 6-phosphoglucono-delta-lactone, pH 7.0, 25°C Plasmodium falciparum
3.1.1.31 1065
-
recombinant h6PGL, substrate is 6-phospho-D-glucono-1,5-lactone, pH 7.0, 25°C Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.31 6-phospho-D-glucono-1,4-lactone + H2O the gamma-lactone is more stable than the natural delta-lactone substrate. It contains a five-membered heterocyclic ring, whereas the delta-lactone contains a six-membered heterocyclic ring Plasmodium falciparum 6-phospho-D-gluconate
-
?
3.1.1.31 6-phospho-D-glucono-1,4-lactone + H2O the gamma-lactone is more stable than the natural delta-lactone substrate. It contains a five-membered heterocyclic ring, whereas the delta-lactone contains a six-membered heterocyclic ring Homo sapiens 6-phospho-D-gluconate
-
?
3.1.1.31 6-phospho-D-glucono-1,5-lactone + H2O
-
Plasmodium falciparum 6-phospho-D-gluconate
-
?
3.1.1.31 6-phospho-D-glucono-1,5-lactone + H2O
-
Homo sapiens 6-phospho-D-gluconate
-
?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.31 6-phosphogluconolactonase
-
Homo sapiens
3.1.1.31 6PGL
-
Homo sapiens
3.1.1.31 glucose-6-phosphate dehydrogenase-6-phosphogluconolactonase
-
Plasmodium falciparum
3.1.1.31 GluPho
-
Plasmodium falciparum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.31 25
-
assay at Plasmodium falciparum
3.1.1.31 25
-
assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.31 7
-
assay at Plasmodium falciparum
3.1.1.31 7
-
assay at Homo sapiens

General Information

EC Number General Information Comment Organism
3.1.1.31 metabolism in Plasmodium falciparum, glucose-6-phosphate dehydrogenase is combined with the second enzyme of the pentose phosphate pathway to create a unique bifunctional enzyme named GluPho, glucose-6-phosphate dehydrogenase/6-phosphogluconolactonase. The G6PD activity is not largely influenced by the 6PGL part Plasmodium falciparum