EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.1.31 | cloning of the gene encoding PfGluPho from chromosome 14 of the 3D7 gametocyte cDNA library, heterologous overexpression of PfGluPho using vector pQE30 in Escherichia coli strain M15 | Plasmodium falciparum |
3.1.1.31 | gene h6PGL, overexpression as N-terminally His-tagged enzyme in Escherichia coli strain BL21 using vector pRAREII | Homo sapiens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.31 | glutathione | PfGluPho is inhibited by S-glutathionylation | Plasmodium falciparum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.31 | additional information | - |
additional information | detailed kinetic analysis of the bifunctional glucose-6-phosphate dehydrogenase/6-phosphogluconolactonase indicates that PfGluPho functions on the basis of a rapid equilibrium random Bi Bi mechanism, where the binding of the second substrate depends on the first substrate | Plasmodium falciparum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.31 | 6-phospho-D-glucono-1,5-lactone + H2O | Plasmodium falciparum | - |
6-phospho-D-gluconate | - |
? | |
3.1.1.31 | 6-phospho-D-glucono-1,5-lactone + H2O | Homo sapiens | - |
6-phospho-D-gluconate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.1.31 | Homo sapiens | O95336 | - |
- |
3.1.1.31 | Plasmodium falciparum | - |
- |
- |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
3.1.1.31 | 6-phospho-D-glucono-1,5-lactone + H2O = 6-phospho-D-gluconate | PfGluPho functions on the basis of a rapid equilibrium random Bi Bi mechanism, where the binding of the second substrate depends on the first substrate | Plasmodium falciparum |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.1.31 | 46 | - |
recombinant PfGluPho, substrate is 6-phosphoglucono-gamma-lactone, pH 7.0, 25°C | Plasmodium falciparum |
3.1.1.31 | 60 | - |
recombinant PfGluPho, substrate is 6-phosphoglucono-delta-lactone, pH 7.0, 25°C | Plasmodium falciparum |
3.1.1.31 | 1065 | - |
recombinant h6PGL, substrate is 6-phospho-D-glucono-1,5-lactone, pH 7.0, 25°C | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.31 | 6-phospho-D-glucono-1,4-lactone + H2O | the gamma-lactone is more stable than the natural delta-lactone substrate. It contains a five-membered heterocyclic ring, whereas the delta-lactone contains a six-membered heterocyclic ring | Plasmodium falciparum | 6-phospho-D-gluconate | - |
? | |
3.1.1.31 | 6-phospho-D-glucono-1,4-lactone + H2O | the gamma-lactone is more stable than the natural delta-lactone substrate. It contains a five-membered heterocyclic ring, whereas the delta-lactone contains a six-membered heterocyclic ring | Homo sapiens | 6-phospho-D-gluconate | - |
? | |
3.1.1.31 | 6-phospho-D-glucono-1,5-lactone + H2O | - |
Plasmodium falciparum | 6-phospho-D-gluconate | - |
? | |
3.1.1.31 | 6-phospho-D-glucono-1,5-lactone + H2O | - |
Homo sapiens | 6-phospho-D-gluconate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.1.31 | 6-phosphogluconolactonase | - |
Homo sapiens |
3.1.1.31 | 6PGL | - |
Homo sapiens |
3.1.1.31 | glucose-6-phosphate dehydrogenase-6-phosphogluconolactonase | - |
Plasmodium falciparum |
3.1.1.31 | GluPho | - |
Plasmodium falciparum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.31 | 25 | - |
assay at | Plasmodium falciparum |
3.1.1.31 | 25 | - |
assay at | Homo sapiens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.31 | 7 | - |
assay at | Plasmodium falciparum |
3.1.1.31 | 7 | - |
assay at | Homo sapiens |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.1.1.31 | metabolism | in Plasmodium falciparum, glucose-6-phosphate dehydrogenase is combined with the second enzyme of the pentose phosphate pathway to create a unique bifunctional enzyme named GluPho, glucose-6-phosphate dehydrogenase/6-phosphogluconolactonase. The G6PD activity is not largely influenced by the 6PGL part | Plasmodium falciparum |