EC Number | Application | Comment | Organism |
---|---|---|---|
3.2.1.177 | biotechnology | saccharification of cell wall polymers | Cellvibrio japonicus |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.177 | expression in Escherichia coli | Cellvibrio japonicus |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.2.1.177 | crystallographic structures of both the apo enzyme and the trapped covalent 5-fluoro-beta-xylosyl-enzyme intermediate, together with docking studies with the XXXG heptasaccharide the importance of a PA14 domain insert in the recognition of longer oligosaccharides by extension of the active-site pocket | Cellvibrio japonicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.177 | 0.39 | - |
4-nitrophenyl-alpha-D-xylopyranoside | pH 6, 25°C | Cellvibrio japonicus | |
3.2.1.177 | 0.7 | - |
Xyl-alpha-(1->6)-Glc-beta-(1->4)-Glc | pH 6, 25°C | Cellvibrio japonicus | |
3.2.1.177 | 55 | - |
Isoprimeverose | pH 6, 25°C | Cellvibrio japonicus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.2.1.177 | outer membrane | - |
Cellvibrio japonicus | 19867 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.177 | 113643 | - |
x * 113643, calculated from sequence | Cellvibrio japonicus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.177 | Cellvibrio japonicus | B3PBD9 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.177 | - |
Cellvibrio japonicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.177 | 4-nitrophenyl-alpha-D-xylopyranoside + H2O | - |
Cellvibrio japonicus | 4-nitrophenol + alpha-D-xylose | - |
? | |
3.2.1.177 | isoprimeverose + H2O | kcat/KM is 11.5% compared to the value for 4-nitrophenyl-beta-isoprimeveroside | Cellvibrio japonicus | alpha-D-xylose + beta-D-glucose | - |
? | |
3.2.1.177 | additional information | CjXyl31A exhibits high regiospecificity for the hydrolysis of xylogluco-oligosaccharides, with a particular preference for longer substrates | Cellvibrio japonicus | ? | - |
? | |
3.2.1.177 | Xyl-alpha-(1->6)-Glc-beta-(1->4)-Glc + H2O | - |
Cellvibrio japonicus | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.177 | ? | x * 113643, calculated from sequence | Cellvibrio japonicus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.177 | xyl31A | - |
Cellvibrio japonicus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.177 | 25 | - |
assay at | Cellvibrio japonicus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.177 | 35 | - |
4 h, 15% loss of activity | Cellvibrio japonicus |
3.2.1.177 | 45 | - |
4 h, 15% loss of activity | Cellvibrio japonicus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.177 | 0.58 | - |
4-nitrophenyl-alpha-D-xylopyranoside | pH 6, 25°C | Cellvibrio japonicus | |
3.2.1.177 | 2.94 | - |
Isoprimeverose | pH 6, 25°C | Cellvibrio japonicus | |
3.2.1.177 | 9.39 | - |
Xyl-alpha-(1->6)-Glc-beta-(1->4)-Glc | pH 6, 25°C | Cellvibrio japonicus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.177 | 6 | - |
assay at | Cellvibrio japonicus |
3.2.1.177 | 6.5 | - |
- |
Cellvibrio japonicus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.177 | 4.5 | 8.5 | pH 4.5: about 60% of maximal activity, pH 8.5: about 60% of maximal activity | Cellvibrio japonicus |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.2.1.177 | physiological function | the enzyme is involved in xyloglucan saccharification | Cellvibrio japonicus |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.177 | 0.05 | - |
Isoprimeverose | pH 6, 25°C | Cellvibrio japonicus | |
3.2.1.177 | 1.48 | - |
4-nitrophenyl-alpha-D-xylopyranoside | pH 6, 25°C | Cellvibrio japonicus | |
3.2.1.177 | 13.4 | - |
Xyl-alpha-(1->6)-Glc-beta-(1->4)-Glc | pH 6, 25°C | Cellvibrio japonicus |