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Literature summary extracted from

  • Larsbrink, J.; Izumi, A.; Ibatullin, F.; Nakhai, A.; Gilbert, H.J.; Davies, G.J.; Brumer, H.
    Structural and enzymatic characterisation of a glycoside hydrolase family 31 alpha-xylosidase from Cellvibrio japonicus involved in xyloglucan saccharification (2011), Biochem. J., 436, 567-580.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.177 biotechnology saccharification of cell wall polymers Cellvibrio japonicus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.177 expression in Escherichia coli Cellvibrio japonicus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.177 crystallographic structures of both the apo enzyme and the trapped covalent 5-fluoro-beta-xylosyl-enzyme intermediate, together with docking studies with the XXXG heptasaccharide the importance of a PA14 domain insert in the recognition of longer oligosaccharides by extension of the active-site pocket Cellvibrio japonicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.177 0.39
-
4-nitrophenyl-alpha-D-xylopyranoside pH 6, 25°C Cellvibrio japonicus
3.2.1.177 0.7
-
Xyl-alpha-(1->6)-Glc-beta-(1->4)-Glc pH 6, 25°C Cellvibrio japonicus
3.2.1.177 55
-
Isoprimeverose pH 6, 25°C Cellvibrio japonicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.177 outer membrane
-
Cellvibrio japonicus 19867
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.177 113643
-
x * 113643, calculated from sequence Cellvibrio japonicus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.177 Cellvibrio japonicus B3PBD9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.177
-
Cellvibrio japonicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.177 4-nitrophenyl-alpha-D-xylopyranoside + H2O
-
Cellvibrio japonicus 4-nitrophenol + alpha-D-xylose
-
?
3.2.1.177 isoprimeverose + H2O kcat/KM is 11.5% compared to the value for 4-nitrophenyl-beta-isoprimeveroside Cellvibrio japonicus alpha-D-xylose + beta-D-glucose
-
?
3.2.1.177 additional information CjXyl31A exhibits high regiospecificity for the hydrolysis of xylogluco-oligosaccharides, with a particular preference for longer substrates Cellvibrio japonicus ?
-
?
3.2.1.177 Xyl-alpha-(1->6)-Glc-beta-(1->4)-Glc + H2O
-
Cellvibrio japonicus ?
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.177 ? x * 113643, calculated from sequence Cellvibrio japonicus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.177 xyl31A
-
Cellvibrio japonicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.177 25
-
assay at Cellvibrio japonicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.177 35
-
4 h, 15% loss of activity Cellvibrio japonicus
3.2.1.177 45
-
4 h, 15% loss of activity Cellvibrio japonicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.177 0.58
-
4-nitrophenyl-alpha-D-xylopyranoside pH 6, 25°C Cellvibrio japonicus
3.2.1.177 2.94
-
Isoprimeverose pH 6, 25°C Cellvibrio japonicus
3.2.1.177 9.39
-
Xyl-alpha-(1->6)-Glc-beta-(1->4)-Glc pH 6, 25°C Cellvibrio japonicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.177 6
-
assay at Cellvibrio japonicus
3.2.1.177 6.5
-
-
Cellvibrio japonicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.177 4.5 8.5 pH 4.5: about 60% of maximal activity, pH 8.5: about 60% of maximal activity Cellvibrio japonicus

General Information

EC Number General Information Comment Organism
3.2.1.177 physiological function the enzyme is involved in xyloglucan saccharification Cellvibrio japonicus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.177 0.05
-
Isoprimeverose pH 6, 25°C Cellvibrio japonicus
3.2.1.177 1.48
-
4-nitrophenyl-alpha-D-xylopyranoside pH 6, 25°C Cellvibrio japonicus
3.2.1.177 13.4
-
Xyl-alpha-(1->6)-Glc-beta-(1->4)-Glc pH 6, 25°C Cellvibrio japonicus