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Literature summary extracted from

  • Honda, Y.; Kitaoka, M.;, Hayashi, K.
    Reaction mechanism of chitobiose phosphorylase from Vibrio proteolyticus: identification of family 36 glycosyltransferase in Vibrio (2004), Biochem. J., 377, 225-232.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.280 expression in Escherichia coli Vibrio proteolyticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.280 2
-
 N,N'-diacetylchitobiose pH 7.0, 30°C Vibrio proteolyticus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.280 cytosol
-
 Vibrio proteolyticus 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.280 additional information no requirement for divalent cations Vibrio proteolyticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.280 90000
-
 2 * 90000, SDS-PAGE Vibrio proteolyticus
2.4.1.280 90360
-
 calculated from amino acid sequence Vibrio proteolyticus
2.4.1.280 150000
-
 gel filtration Vibrio proteolyticus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.280 N,N'-diacetylchitobiose + phosphate Vibrio proteolyticus
-
 N-acetyl-D-glucosamine + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 r
2.4.1.280 N,N'-diacetylchitobiose + phosphate Vibrio proteolyticus
-
 N-acetyl-D-glucosamine + N-acetyl-alpha-D-glucosamine 1-phosphate inversion of the anomeric configuration r

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.280 Vibrio proteolyticus Q76IQ9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.280
-
 Vibrio proteolyticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.280 additional information no substrates are N-acetylchitooligosaccharides Vibrio proteolyticus ?
-
 ?
2.4.1.280 N,N'-diacetylchitobiose + phosphate
-
 Vibrio proteolyticus N-acetyl-D-glucosamine + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 r
2.4.1.280 N,N'-diacetylchitobiose + phosphate
-
 Vibrio proteolyticus N-acetyl-D-glucosamine + N-acetyl-alpha-D-glucosamine 1-phosphate inversion of the anomeric configuration r
2.4.1.280 N-acetyl-alpha-D-glucosamine 1-phosphate + 4-methylumbelliferyl N-acetyl-beta-D-glucosaminide reverse phosphorolysis, reaction at 20% the rate of N-acetyl-D-glucosamine Vibrio proteolyticus 4-methylumbelliferyl N,N'-diacetylchitobioside + phosphate
-
 r
2.4.1.280 N-acetyl-alpha-D-glucosamine 1-phosphate + 4-nitrophenyl N-acetyl-beta-D-glucosaminide reverse phosphorolysis, reaction at 10% the rate of N-acetyl-D-glucosamine Vibrio proteolyticus 4-nitrophenyl N,N'-diacetylchitobioside + phosphate
-
 r
2.4.1.280 N-acetyl-D-glucosamine + N-acetyl-alpha-D-glucosamine 1-phosphate reverse phosphorolysis reaction Vibrio proteolyticus N,N'-diacetylchitobiose + phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.4.1.280 dimer 2 * 90000, SDS-PAGE Vibrio proteolyticus

Synonyms

EC Number Synonyms Comment Organism
2.4.1.280 chbP
-
 Vibrio proteolyticus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.280 30
-
-
 Vibrio proteolyticus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.280 35
-
 stable up to Vibrio proteolyticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.280 7 7.7
-
 Vibrio proteolyticus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.280 7 8.1
-
 Vibrio proteolyticus