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Literature summary extracted from

  • Misra, K.; Banerjee, A.B.; Ray, S.; Ray, M.
    Glyoxalase III from Escherichia coli: a single novel enzyme for the conversion of methylglyoxal into D-lactate without reduced glutathione (1995), Biochem. J., 305, 999-1003.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
4.2.1.130 DTT and either sucrose or mannitol are required for stability Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.130 4-hydroxymercuribenzoate inactivates the enzyme, reversible by dithiothreitol and other thiol-group-containing compounds Escherichia coli
4.2.1.130 5,5'-dithiobis-(2-nitrobenzoate) 0.4 mM, 15 min, complete inactivation. 5 mM DTT restores activity almost completely Escherichia coli
4.2.1.130 Borate boric acid/borate buffer is moderately inhibitory Escherichia coli
4.2.1.130 DTNB
-
 Escherichia coli
4.2.1.130 additional information glutathione analogues, which are inhibitors of glyoxalase I, do not inhibit glyoxalase III, but the enzyme is sensitive to thiol-blocking reagents; S-methylglutathione and S-octylglutathione, even at concentrations of 20 mM and 0.25 mM respectively, have no inhibitory effect on Escherichia coli glyoxalase III Escherichia coli
4.2.1.130 N-ethylmaleimide 0.4 mM, 15 min, complete inactivation. 5 mM DTT restores activity almost completely Escherichia coli
4.2.1.130 NEM
-
 Escherichia coli
4.2.1.130 p-hydroxymercuribenzoate 0.4 mM, 15 min, complete inactivation. 5 mM DTT restores activity almost completely Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.130 40000
-
 2 * 40000, SDS-PAGE Escherichia coli
4.2.1.130 41000
-
-
 Escherichia coli
4.2.1.130 82000
-
 gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.130 2-oxopropanal + H2O Escherichia coli
-
 (R)-lactate
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.130 Escherichia coli
-
-
-
4.2.1.130 Escherichia coli P31658
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.130
-
 Escherichia coli
4.2.1.130 native enzyme 725fold by ammonium sulfate fractionation, heat treatment, gel filtration, anion exchange chromatography, and affinity chromatography on a 4-hydroxymercuribenzoate-bound resin Escherichia coli

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.1.130 additional information glyoxalase III activity is present in all three media in almost equal amounts ranging from 0.033 to 0.054 unit/mg of protein Escherichia coli
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.130 0.033 0.054 crude enzyme extracts, pH 8.0, 37°C Escherichia coli
4.2.1.130 37
-
 pH 8.0, 37°C Escherichia coli

Storage Stability

EC Number Storage Stability Organism
4.2.1.130 -15°C, 1 mM DTT and 10% sucrose, almost completely stable Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.130 2-oxopropanal + H2O
-
 Escherichia coli (R)-lactate
-
 ir
4.2.1.130 2-oxopropanal + H2O stereospcific recation Escherichia coli (R)-lactate
-
 ir
4.2.1.130 methylglyoxal + H2O glyoxalase III does not catalyse the reverse reaction. Glyoxalase III cannot catalyse either the formation or the breakdown of S-D-lactoylglutathione Escherichia coli (R)-lactate
-
 ir
4.2.1.130 additional information purified glyoxalase III can catalyse the conversion of methylglyoxal into S-D-lactoylglutathione in the presence of GSH or S-D-lactoylglutathione into D-lactate, cf. EC 4.4.1.5 Escherichia coli ?
-
 ?
4.2.1.130 phenylglyoxal + H2O
-
 Escherichia coli ?
-
 ir
4.2.1.130 phenylglyoxal + H2O the rate with phenylglyoxal is about 15% of that with methylglyoxal Escherichia coli ?
-
 ir

Subunits

EC Number Subunits Comment Organism
4.2.1.130 dimer 2 * 40000, SDS-PAGE Escherichia coli
4.2.1.130 dimer 2 * 41000, about, SDS-PAGE Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
4.2.1.130 glyoxalase III
-
 Escherichia coli
4.2.1.130 glyoxylase III
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.130 37
-
 assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.130 6 8 no sharp optimum Escherichia coli
4.2.1.130 8
-
 assay at Escherichia coli
4.2.1.130 8
-
 glyoxalase III is active over a wide range of pH with no sharp pH optimum Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.1.130 5 10 pH 5.0: sharp decrease in reaction rate below, pH 10.0: activity 20-25% lower than the rate at physiological pH Escherichia coli
4.2.1.130 5 10 glyoxalase III is active over a wide range of pH with no sharp pH optimum, sharp decrease in reaction rate occurs below pH 5.0, 20.25% of maximal activity at pH 10.0 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
4.2.1.130 additional information no requirement for glutathione, GSH Escherichia coli