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Literature summary extracted from

  • Oppenheimer, M.; Valenciano, A.L.; Sobrado, P.
    Isolation and characterization of functional Leishmania major virulence factor UDP-galactopyranose mutase (2011), Biochem. Biophys. Res. Commun., 407, 552-556.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.4.99.9 expressed in Escherichia coli BL21T1 and BL21(DE3) cells Leishmania major

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.4.99.9 0.087
-
UDP-D-galactofuranose in 25 mM HEPES, 125 mM NaCl, 20 mM dithionite, pH 7.5, at 37°C Leishmania major

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.4.99.9 54960
-
1 * 54960, calculated from amino acid sequence Leishmania major
5.4.99.9 55000
-
1 * 55000, SDS-PAGE Leishmania major

Organism

EC Number Organism UniProt Comment Textmining
5.4.99.9 Leishmania major
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.4.99.9 nickel immobilized metal affinity chromatography, DEAE column chromatography, and purification with HaloLink resin Leishmania major

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.99.9 additional information the enzyme is active only in the reduced state Leishmania major ?
-
?
5.4.99.9 UDP-D-galactofuranose
-
Leishmania major UDP-D-galactopyranose
-
r
5.4.99.9 UDP-D-galactopyranose
-
Leishmania major UDP-D-galactofuranose
-
r

Subunits

EC Number Subunits Comment Organism
5.4.99.9 monomer 1 * 55000, SDS-PAGE Leishmania major
5.4.99.9 monomer 1 * 54960, calculated from amino acid sequence Leishmania major

Synonyms

EC Number Synonyms Comment Organism
5.4.99.9 UGM
-
Leishmania major

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.4.99.9 5
-
UDP-D-galactofuranose in 25 mM HEPES, 125 mM NaCl, 20 mM dithionite, pH 7.5, at 37°C Leishmania major

Cofactor

EC Number Cofactor Comment Organism Structure
5.4.99.9 FAD
-
Leishmania major

General Information

EC Number General Information Comment Organism
5.4.99.9 physiological function UDP-galactopyranose mutase is a virulence factor in Leishmania major Leishmania major

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.4.99.9 57
-
UDP-D-galactofuranose in 25 mM HEPES, 125 mM NaCl, 20 mM dithionite, pH 7.5, at 37°C Leishmania major