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Literature summary extracted from

  • Takahashi-Iniguez, T.; Garcia-Arellano, H.; Trujillo-Roldan, M.A.; Flores, M.E.
    Protection and reactivation of human methylmalonyl-CoA mutase by MMAA protein (2011), Biochem. Biophys. Res. Commun., 404, 443-447.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
5.4.99.2 methylmalonic acidemia protein after 60 min of reaction, when MCM is inactive, the addition of methylmalonic acidemia protein increases the enzymatic activity through GTP hydrolysis, indicating reactivation of MCM by exchange of the damaged cofactor. Methylmalonic acidemia protein acts as a chaperone of human MCM Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.4.99.2 expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.4.99.2 80000
-
 x * 80000, SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
5.4.99.2 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.4.99.2 IMAC column chromatography Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.99.2 (R)-2-methylmalonyl-CoA
-
 Homo sapiens succinyl-CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
5.4.99.2 ? x * 80000, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
5.4.99.2 MCM
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
5.4.99.2 adenosylcobalamin MCM activity with adenosylcobalamin increases linearly during the first 3-4 min Homo sapiens