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Literature summary extracted from
- Efficient hydrolysis of the chemical warfare nerve agent tabun by recombinant and purified human and rabbit serum paraoxonase 1 (2010), Biochem. Biophys. Res. Commun., 403, 97-102.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.1.8.1 | medicine | PON1 shows potential as a prophylactic treatment against tabun exposure | Homo sapiens |
| 3.1.8.1 | medicine | PON1 shows potential as a prophylactic treatment against tabun exposure | Oryctolagus cuniculus |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.1.2 | expressed in Trichoplusia ni larvae | Homo sapiens |
| 3.1.8.1 | expressed in Trichoplusia ni larvae | Homo sapiens |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.2 | 2-hydroxyquinoline | specific paraoxonase 1 inhibitor, dose-dependent inhibition | Homo sapiens |  |
| 3.1.1.2 | 2-hydroxyquinoline | specific paraoxonase 1 inhibitor, dose-dependent inhibition | Oryctolagus cuniculus | |
| 3.1.8.1 | 2-hydroxyquinoline | specific paraoxonase 1 inhibitor, dose-dependent inhibition | Homo sapiens | |
| 3.1.8.1 | 2-hydroxyquinoline | specific paraoxonase 1 inhibitor, dose-dependent inhibition | Oryctolagus cuniculus | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.1.2 | Homo sapiens | - |
- |
- |
| 3.1.1.2 | Oryctolagus cuniculus | - |
- |
- |
| 3.1.8.1 | Homo sapiens | - |
- |
- |
| 3.1.8.1 | Oryctolagus cuniculus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.1.2 | DEAE-Sepharose column chromatography, Concanavalin A-Sepharose column chromatography, and Sepharose CL-6B gel filtration | Homo sapiens |
| 3.1.1.2 | DEAE-Sepharose column chromatography, Concanavalin A-Sepharose column chromatography, and Sepharose CL-6B gel filtration | Oryctolagus cuniculus |
| 3.1.8.1 | DEAE-Sepharose column chromatography, Concanavalin A-Sepharose column chromatography, and Sepharose CL-6B gel filtration | Homo sapiens |
| 3.1.8.1 | DEAE-Sepharose column chromatography, Concanavalin A-Sepharose column chromatography, and Sepharose CL-6B gel filtration | Oryctolagus cuniculus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.1.2 | serum | - |
Homo sapiens | - |
| 3.1.1.2 | serum | - |
Oryctolagus cuniculus | - |
| 3.1.8.1 | serum | - |
Homo sapiens | - |
| 3.1.8.1 | serum | - |
Oryctolagus cuniculus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.2 | 3.5 | - |
enzyme purified from serum, in 20 mM Tris-HCl buffer (pH 7.4), at 37°C | Homo sapiens |
| 3.1.1.2 | 3.5 | - |
enzyme purified from serum, in 20 mM Tris-HCl buffer (pH 7.4), at 37°C | Oryctolagus cuniculus |
| 3.1.1.2 | 8.5 | - |
recombinant enzyme, after purification, in 20 mM Tris-HCl buffer (pH 7.4), at 37°C | Homo sapiens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.2 | 4-nitrophenyl acetate + H2O | - |
Homo sapiens | 4-nitrophenol + acetate | - |
? | |
| 3.1.1.2 | 4-nitrophenyl acetate + H2O | - |
Oryctolagus cuniculus | 4-nitrophenol + acetate | - |
? | |
| 3.1.8.1 | diisopropyl fluorophosphate + H2O | - |
Homo sapiens | ? | - |
? | |
| 3.1.8.1 | diisopropyl fluorophosphate + H2O | - |
Oryctolagus cuniculus | ? | - |
? | |
| 3.1.8.1 | sarin + H2O | efficient hydrolysis of sarin (11 nM) is observed with about 0.08-0.25 units of paraoxonase 1 | Homo sapiens | ? | - |
? | |
| 3.1.8.1 | sarin + H2O | efficient hydrolysis of sarin (11 nM) is observed with about 0.08-0.25 units of paraoxonase 1 | Oryctolagus cuniculus | ? | - |
? | |
| 3.1.8.1 | soman + H2O | efficient hydrolysis of soman (3 nM) is observed with about 0.08-0.25 units of paraoxonase 1 | Homo sapiens | ? | - |
? | |
| 3.1.8.1 | soman + H2O | efficient hydrolysis of soman (3 nM) is observed with about 0.08-0.25 units of paraoxonase 1 | Oryctolagus cuniculus | ? | - |
? | |
| 3.1.8.1 | tabun + H2O | efficient hydrolysis of tabun (100 nM) is observed with about 0.025-0.04 units of paraoxonase 1. Tabun hydrolysis with paraoxonase 1 is about 30-60times and about 200-260times more efficient than that with sarin and soman, respectively | Homo sapiens | ? | - |
? | |
| 3.1.8.1 | tabun + H2O | efficient hydrolysis of tabun (100 nM) is observed with about 0.025-0.04 units of paraoxonase 1. Tabun hydrolysis with paraoxonase 1 is about 30-60times and about 200-260times more efficient than that with sarin and soman, respectively | Oryctolagus cuniculus | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.1.2 | ? | x * 40000-45000, paraoxonase 1 is separated as three bands with an approximate molecular weight of 40000-45000 Da, SDS-PAGE | Homo sapiens |
| 3.1.1.2 | ? | x * 40000-45000, paraoxonase 1 is separated as three bands with an approximate molecular weight of 40000-45000 Da, SDS-PAGE | Oryctolagus cuniculus |
| 3.1.8.1 | ? | x * 40000-45000, paraoxonase 1 is separated as three bands with an approximate molecular weight of 40000-45000 Da, SDS-PAGE | Homo sapiens |
| 3.1.8.1 | ? | x * 40000-45000, paraoxonase 1 is separated as three bands with an approximate molecular weight of 40000-45000 Da, SDS-PAGE | Oryctolagus cuniculus |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 3.1.8.1 | physiological function | paraoxonase 1 functions as an efficient catalytic bioscavenger against nerve agents | Homo sapiens |
| 3.1.8.1 | physiological function | paraoxonase 1 functions as an efficient catalytic bioscavenger against nerve agents | Oryctolagus cuniculus |
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Release 2023.1 (January 2023)
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