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Literature summary extracted from
- The structure of Aspergillus niger phytase PhyA in complex with a phytate mimetic (2010), Biochem. Biophys. Res. Commun., 397, 745-749.
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 3.1.3.8 | deglycosylated PhyA free or in complex with inhibitor myo-inositol-1,2,3,4,5,6-hexakis sulfate, vapour-diffusion hanging drop technique, reservoirs contain 1 ml of PEG 3350 at 15-30% w/v, and ammonium nitrate a t0.1-0.4 M, siliconized coverslips are used to suspend a drop containing 0.002 ml of phytase at 13 mg/ml protein concetration and 0.002 ml of reservoir solution over the reservoir, X-ray diffraction structure determination and analysis at 2.2-2.4 A resolution | Aspergillus niger |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.3.8 | myo-inositol-1,2,3,4,5,6-hexakissulfate | potent inhibition, the compound is isosteric and isoelectronic with respect to substrate myo-inositol-1,2,3,4,5,6-hexakisphosphate | Aspergillus niger |  |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.1.3.8 | extracellular | - |
Aspergillus niger | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.8 | 47000 | - |
x * 60000, glycosylated enzyme, SDS-PAGE, x * 47000, deglycosylated enzyme, SDS-PAGE | Aspergillus niger |
| 3.1.3.8 | 60000 | - |
x * 60000, glycosylated enzyme, SDS-PAGE, x * 47000, deglycosylated enzyme, SDS-PAGE | Aspergillus niger |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.3.8 | myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O | Aspergillus niger | - |
? + phosphate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.3.8 | Aspergillus niger | - |
gene phyA | - |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.3.8 | commercial preparation | - |
Aspergillus niger | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.3.8 | myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O | - |
Aspergillus niger | ? + phosphate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.3.8 | ? | x * 60000, glycosylated enzyme, SDS-PAGE, x * 47000, deglycosylated enzyme, SDS-PAGE | Aspergillus niger |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.3.8 | myo-inositol-hexaphosphate phosphohydrolase | - |
Aspergillus niger |
| 3.1.3.8 | PhyA | - |
Aspergillus niger |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.8 | 5 | 5.5 | - |
Aspergillus niger |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.8 | 2.5 | 5.5 | pH-activity profile, with optima at pH 2.5 with 60% of maximal activity, and at pH 5.0-5.5 with maximal activity, and a local minimum at pH 3.5 | Aspergillus niger |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.3.8 | 0.0046 | - |
myo-inositol-1,2,3,4,5,6-hexakissulfate | pH 5.0-5.5, temperature not specified in the publication | Aspergillus niger | |
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Release 2023.1 (January 2023)
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