Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Momoi, K.; Hofmann, U.; Schmid, R.; Urlacher, V.
    Reconstitution of beta-carotene hydroxylase activity of thermostable CYP175A1 monooxygenase (2006), Biochem. Biophys. Res. Commun., 339, 331-336.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.15.24 expressed in Escherichia coli BL21(DE3) cells Thermus thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.15.24 47000
-
 SDS-PAGE Thermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.24 Thermus thermophilus
-
-
-
1.14.15.24 Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.15.24 ammonium sulfate precipitation, phenyl Sepharose column chromatography, and hydroxyapatite column chromatography Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.24 beta-carotene + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2 upon in vitro reconstitution of CYP175A1 activity with purified recombinant flavodoxin and flavodoxin reductase from Escherichia coli, only very low beta-cryptoxanthin production is observed Thermus thermophilus beta-cryptoxanthin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.24 beta-carotene + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2 upon in vitro reconstitution of CYP175A1 activity with purified recombinant flavodoxin and flavodoxin reductase from Escherichia coli, only very low beta-cryptoxanthin production is observed Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 beta-cryptoxanthin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.24 beta-carotene + 4 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + 2 O2 overall reaction, the enzyme catalyzes the hydroxylation of beta-carotene at 3- and 3'-positions Thermus thermophilus zeaxanthin + 4 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
 ?
1.14.15.24 beta-carotene + 4 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + 2 O2 overall reaction, the enzyme catalyzes the hydroxylation of beta-carotene at 3- and 3'-positions Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 zeaxanthin + 4 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
 ?
1.14.15.24 beta-cryptoxanthin + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
 Thermus thermophilus zeaxanthin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.24 beta-cryptoxanthin + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
 Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 zeaxanthin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.15.24 CYP175A1
-
 Thermus thermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.14.15.24 65
-
 the enzyme activity disappears completely at 65°C Thermus thermophilus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.15.24 heme
-
 Thermus thermophilus