Literature summary extracted from

Tsigelny, I.; Baker, M.E.
Structures important in NAD(P)(H) specificity for mammalian retinol and 11-cis-retinol dehydrogenases (1996), Biochem. Biophys. Res. Commun., 226, 118-127.

Crystallization (Commentary)

EC Number	Crystallization (Comment)	Organism
1.1.1.300	alignment of Streptomyces hydrogenans 20beta-hydroxysteroid dehydrogenase with rat retinol dehydrogenase and cow 11-cis retinol dehydrogenase. Lysine64 of the rat enzyme is important in stabilizing binding of 2'-phosphate on NADP+ in two ways: lysine's positively charged side chain has a coulombic attraction to the 2'-phosphate and partially compensates for the negative charge of aspartic acid-38. Cow 11-cis retinol dehydrogenase has threonine61 at the position homologous to lysine64. Threonine61 does not have a stabilizing coulombic interaction with NADP+, nor can threonine61 counteract the repulsive interaction between NADP+ and aspartic acid37 in 11-cis retinol dehydrogenase	Rattus norvegicus
1.1.1.315	alignment of Streptomyces hydrogenans 20beta-hydroxysteroid dehydrogenase with rat retinol dehydrogenase and cow 11-cis retinol dehydrogenase. Lysine64 of the rat enzyme is important in stabilizing binding of 2'-phosphate on NADP+ in two ways: lysine's positively charged side chain has a coulombic attraction to the 2'-phosphate and partially compensates for the negative charge of aspartic acid-38. Cow 11-cis retinol dehydrogenase has threonine61 at the position homologous to lysine64. Threonine61 does not have a stabilizing coulombic interaction with NADP+, nor can threonine61 counteract the repulsive interaction between NADP+ and aspartic acid37 in 11-cis retinol dehydrogenase	Bos taurus

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.1.1.300	Rattus norvegicus	-	-	-
1.1.1.315	Bos taurus	-	-	-

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
1.1.1.300	NADP+	lysine64 is important in the specificity of 11-cis retinol dehydrogenase for NADP+	Rattus norvegicus
1.1.1.315	NAD+	aspartic acid37 and threonine61 are important in the specificity of 11-cis retinol dehydrogenase for NAD+	Bos taurus

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Release 2023.1 (January 2023)