Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Nuebel, T.; Klughammer, C.; Huber, R.; Hauska, G.; Schuetz, M.
    Sulfide:quinone oxidoreductase in membranes of the hyperthermophilic bacterium Aquifex aeolicus (VF5) (2000), Arch. Microbiol., 173, 233-244.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.5.4 2-heptylquinolin-4-ol 1-oxide
-
 Aquifex aeolicus
1.8.5.4 2-n-nonyl-4-hydroxyquinoline-N-oxide
-
 Aquifex aeolicus
1.8.5.4 antimycin A
-
 Aquifex aeolicus
1.8.5.4 aurachin C
-
 Aquifex aeolicus
1.8.5.4 additional information insensitive towards cyanide Aquifex aeolicus
1.8.5.4 myxothiazole
-
 Aquifex aeolicus
1.8.5.4 Stigmatellin
-
 Aquifex aeolicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.5.4 0.005
-
 decylubiquinone in 50 mM Bis-Tris (pH 7.0), at 20°C Aquifex aeolicus
1.8.5.4 0.011
-
 Sulfide in 50 mM Bis-Tris (pH 7.0), at 20°C Aquifex aeolicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.8.5.4 membrane
-
 Aquifex aeolicus 16020
-

Organism

EC Number Organism UniProt Comment Textmining
1.8.5.4 Aquifex aeolicus
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.5.4 3.5
-
 in 50 mM Bis-Tris (pH 7.0), at 20°C Aquifex aeolicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.5.4 sulfide + decylubiquinone
-
 Aquifex aeolicus sulfur + decylubiquinol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.8.5.4 SQR
-
 Aquifex aeolicus
1.8.5.4 sulfide:quinone oxidoreductase
-
 Aquifex aeolicus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.8.5.4 20 70 with 0.015 mM decylubqiuinone reduced/mg protein/min, the activity at 70°C is 5fold higher than the activity at 20°C Aquifex aeolicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.8.5.4 95
-
 the reaction with membranes that have been heated for more than 2 h at 95°C is negligible Aquifex aeolicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.5.4 FAD
-
 Aquifex aeolicus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.8.5.4 0.000014
-
 in 50 mM Bis-Tris (pH 7.0), at 20°C Aquifex aeolicus aurachin C
1.8.5.4 0.006
-
 in 50 mM Bis-Tris (pH 7.0), at 20°C Aquifex aeolicus 2-n-nonyl-4-hydroxyquinoline-N-oxide
1.8.5.4 0.01
-
 in 50 mM Bis-Tris (pH 7.0), at 20°C Aquifex aeolicus antimycin A
1.8.5.4 0.012
-
 in 50 mM Bis-Tris (pH 7.0), at 20°C Aquifex aeolicus 2-heptylquinolin-4-ol 1-oxide
1.8.5.4 0.02
-
 in 50 mM Bis-Tris (pH 7.0), at 20°C Aquifex aeolicus Stigmatellin
1.8.5.4 0.043
-
 in 50 mM Bis-Tris (pH 7.0), at 20°C Aquifex aeolicus myxothiazole

General Information

EC Number General Information Comment Organism
1.8.5.4 physiological function SQR and the cytochrome bc complex are involved in sulfide-dependent respiration. Oxidation of sulfide by SQR is coupled, at least in part, to the proton-motive Q-cycle mechanism Aquifex aeolicus