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Literature summary extracted from
- Characterization of recombinant UDP-galactopyranose mutase from Aspergillus fumigatus (2010), Arch. Biochem. Biophys., 502, 31-38.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.4.99.9 | expressed in Escherichia coli BL21T1 cells | Aspergillus fumigatus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.99.9 | 0.11 | - |
UDP-D-galactofuranose | in 25 mM HEPES, 125 mM NaCl, 20 mM dithionite, pH 7.5, at 37°C | Aspergillus fumigatus |  |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.9 | 55000 | - |
4 * 55000, SDS-PAGE | Aspergillus fumigatus |
| 5.4.99.9 | 57152 | - |
4 * 57152, calculated from amino acid sequence | Aspergillus fumigatus |
| 5.4.99.9 | 228600 | - |
calculated from amino acid sequence | Aspergillus fumigatus |
| 5.4.99.9 | 275000 | - |
gel filtration | Aspergillus fumigatus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.4.99.9 | Aspergillus fumigatus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.4.99.9 | nickel immobilized affinity chromatography and DEAE column chromatography | Aspergillus fumigatus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.99.9 | additional information | the enzyme is active in the oxidized state, being 2-3fold less active than in the reduced state. In the oxidized state, UGM does not bind UDP-galactopyranose | Aspergillus fumigatus | ? | - |
? | |
| 5.4.99.9 | UDP-D-galactofuranose | - |
Aspergillus fumigatus | UDP-D-galactopyranose | - |
r | |
| 5.4.99.9 | UDP-D-galactopyranose | - |
Aspergillus fumigatus | UDP-D-galactofuranose | - |
r | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.4.99.9 | homotetramer | 4 * 55000, SDS-PAGE | Aspergillus fumigatus |
| 5.4.99.9 | homotetramer | 4 * 57152, calculated from amino acid sequence | Aspergillus fumigatus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.4.99.9 | UGM | - |
Aspergillus fumigatus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.99.9 | 72 | - |
UDP-D-galactofuranose | in 25 mM HEPES, 125 mM NaCl, 20 mM dithionite, pH 7.5, at 37°C | Aspergillus fumigatus | |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.4.99.9 | FAD | - |
Aspergillus fumigatus | |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.99.9 | 650 | - |
UDP-D-galactofuranose | in 25 mM HEPES, 125 mM NaCl, 20 mM dithionite, pH 7.5, at 37°C | Aspergillus fumigatus | |
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