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Literature summary extracted from

  • Garcia-Hidalgo, J.; Hormigo, D.; Prieto, M.A.; Arroyo, M.; de la Mata, I.
    Extracellular production of Streptomyces exfoliatus poly(3-hydroxybutyrate) depolymerase in Rhodococcus sp. T104: determination of optimal biocatalyst conditions (2012), Appl. Microbiol. Biotechnol., 93, 1975-1988.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.75 gene phaZSex, expression in Rhodococcus sp. strain T104 as an extracellular active enzyme, subcloning in Escherichia coli strain DH5alpha, expression in Escherichia coli strain BL21 (DE3) Streptomyces exfoliatus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.75 EDTA
-
 Streptomyces exfoliatus
3.1.1.75 Mn2+
-
 Streptomyces exfoliatus
3.1.1.75 NaCl complete inhibition at 3 M Streptomyces exfoliatus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.75 extracellular
-
 Streptomyces exfoliatus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.75 Ca2+ activates Streptomyces exfoliatus
3.1.1.75 Mg2+ activates Streptomyces exfoliatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.75 poly(3-hydroxybutyrate) + H2O Streptomyces exfoliatus
-
 ?
-
 ?
3.1.1.75 poly(3-hydroxybutyrate) + H2O Streptomyces exfoliatus DSM Z 41693
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.75 Streptomyces exfoliatus
-
 gene phaZSex
-
3.1.1.75 Streptomyces exfoliatus DSM Z 41693
-
 gene phaZSex
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.75 recombinant enzyme from Rhodococcus sp. strain T104 cell culture medium to homogeneity Streptomyces exfoliatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.75 4-nitrophenyl acetate + H2O
-
 Streptomyces exfoliatus 4-nitrophenol + acetate
-
 ?
3.1.1.75 4-nitrophenyl acetate + H2O
-
 Streptomyces exfoliatus DSM Z 41693 4-nitrophenol + acetate
-
 ?
3.1.1.75 4-nitrophenyl butyrate + H2O
-
 Streptomyces exfoliatus 4-nitrophenol + butyrate
-
 ?
3.1.1.75 4-nitrophenyl butyrate + H2O
-
 Streptomyces exfoliatus DSM Z 41693 4-nitrophenol + butyrate
-
 ?
3.1.1.75 4-nitrophenyl valerate + H2O
-
 Streptomyces exfoliatus 4-nitrophenol + valerate
-
 ?
3.1.1.75 4-nitrophenyl valerate + H2O
-
 Streptomyces exfoliatus DSM Z 41693 4-nitrophenol + valerate
-
 ?
3.1.1.75 poly(3-hydroxybutyrate) + H2O
-
 Streptomyces exfoliatus ?
-
 ?
3.1.1.75 poly(3-hydroxybutyrate) + H2O
-
 Streptomyces exfoliatus DSM Z 41693 ?
-
 ?
3.1.1.75 poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O with 5 or 12 mol% 3-hydroxyvalerate content Streptomyces exfoliatus ?
-
 ?
3.1.1.75 poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O with 5 or 12 mol% 3-hydroxyvalerate content Streptomyces exfoliatus DSM Z 41693 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.75 PHB depolymerase
-
 Streptomyces exfoliatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.75 40
-
 assay at Streptomyces exfoliatus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.75 additional information
-
 unfolding is observed at 55.8°C in a first stage Streptomyces exfoliatus
3.1.1.75 40
-
 the purified enzyme is highly stable at 40°C Streptomyces exfoliatus
3.1.1.75 70
-
 the purified enzyme is completely inactivated after 90 min Streptomyces exfoliatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.75 8
-
 assay at Streptomyces exfoliatus