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Literature summary extracted from
- Characterization of a recombinant cellobiose 2-epimerase from Caldicellulosiruptor saccharolyticus and its application in the production of mannose from glucose (2011), Appl. Microbiol. Biotechnol., 92, 1187-1196.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.1.3.11 | additional information | the enzyme is not affected by ATP | Caldicellulosiruptor saccharolyticus |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.1.3.11 | expressed in Escherichia coli ER2566 cells | Caldicellulosiruptor saccharolyticus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.1.3.11 | 51.8 | - |
D-mannose | in 50 mM PIPES buffer (pH 7.5), at 75°C | Caldicellulosiruptor saccharolyticus |  |
| 5.1.3.11 | 70.9 | - |
D-glucose | in 50 mM PIPES buffer (pH 7.5), at 75°C | Caldicellulosiruptor saccharolyticus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.1.3.11 | 47000 | - |
gel filtration | Caldicellulosiruptor saccharolyticus |
| 5.1.3.11 | 47340 | - |
1 * 47340, calculated from amino acid sequence | Caldicellulosiruptor saccharolyticus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.1.3.11 | Caldicellulosiruptor saccharolyticus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.1.3.11 | His-Trap HP column chromatography, gel filtration | Caldicellulosiruptor saccharolyticus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 5.1.3.11 | 0.0095 | - |
in 50 mM PIPES buffer (pH 7.5) containing 10 mM D-fructose as substrate at 75°C | Caldicellulosiruptor saccharolyticus |
| 5.1.3.11 | 0.035 | - |
in 50 mM PIPES buffer (pH 7.5) containing 10 mM D-glucose as substrate at 75°C | Caldicellulosiruptor saccharolyticus |
| 5.1.3.11 | 0.04 | - |
in 50 mM PIPES buffer (pH 7.5) containing 10 mM D-xylose as substrate at 75°C | Caldicellulosiruptor saccharolyticus |
| 5.1.3.11 | 0.06 | - |
in 50 mM PIPES buffer (pH 7.5) containing 10 mM D-lyxose as substrate at 75°C | Caldicellulosiruptor saccharolyticus |
| 5.1.3.11 | 0.15 | - |
in 50 mM PIPES buffer (pH 7.5) containing 10 mM D-mannose as substrate at 75°C | Caldicellulosiruptor saccharolyticus |
| 5.1.3.11 | 3.6 | - |
in 50 mM PIPES buffer (pH 7.5) containing 10 mM cellobiose as substrate at 75°C | Caldicellulosiruptor saccharolyticus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.1.3.11 | cellobiose | the enzyme activity is approximately 100fold higher for cellobiose than for D-glucose | Caldicellulosiruptor saccharolyticus | 4-O-beta-D-glucopyranosyl-D-mannose | - |
? | |
| 5.1.3.11 | D-fructose | - |
Caldicellulosiruptor saccharolyticus | D-glucose + D-mannose | - |
? | |
| 5.1.3.11 | D-glucose | - |
Caldicellulosiruptor saccharolyticus | D-mannose | - |
? | |
| 5.1.3.11 | D-lyxose | - |
Caldicellulosiruptor saccharolyticus | D-xylose | - |
? | |
| 5.1.3.11 | D-mannose | - |
Caldicellulosiruptor saccharolyticus | D-glucose | - |
? | |
| 5.1.3.11 | D-xylose | - |
Caldicellulosiruptor saccharolyticus | D-lyxose | - |
? | |
| 5.1.3.11 | D-xylulose | low activity | Caldicellulosiruptor saccharolyticus | D-xylose + D-lyxose | - |
? | |
| 5.1.3.11 | L-allose | low activity | Caldicellulosiruptor saccharolyticus | L-altrose | - |
? | |
| 5.1.3.11 | L-altrose | - |
Caldicellulosiruptor saccharolyticus | L-allose | - |
? | |
| 5.1.3.11 | L-arabinose | low activity | Caldicellulosiruptor saccharolyticus | L-ribose | - |
? | |
| 5.1.3.11 | L-gulose | low activity | Caldicellulosiruptor saccharolyticus | L-idose | - |
? | |
| 5.1.3.11 | L-idose | - |
Caldicellulosiruptor saccharolyticus | L-gulose | - |
? | |
| 5.1.3.11 | L-psicose | low activity | Caldicellulosiruptor saccharolyticus | L-allose + L-altrose | - |
? | |
| 5.1.3.11 | L-ribulose | low activity | Caldicellulosiruptor saccharolyticus | L-arabinose + L-ribose | - |
? | |
| 5.1.3.11 | L-sorbose | low activity | Caldicellulosiruptor saccharolyticus | L-gulose + L-idose | - |
? | |
| 5.1.3.11 | mannose | the enzyme exhibits the highest isomerization activity for mannose among monosaccharides | Caldicellulosiruptor saccharolyticus | D-glucose + D-fructose | - |
? | |
| 5.1.3.11 | additional information | the enzyme is not active for N-acyl-D-glucosamine | Caldicellulosiruptor saccharolyticus | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.1.3.11 | monomer | 1 * 47000, SDS-PAGE | Caldicellulosiruptor saccharolyticus |
| 5.1.3.11 | monomer | 1 * 47340, calculated from amino acid sequence | Caldicellulosiruptor saccharolyticus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.1.3.11 | cellobiose 2-epimerase | - |
Caldicellulosiruptor saccharolyticus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.1.3.11 | 75 | - |
- |
Caldicellulosiruptor saccharolyticus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.1.3.11 | 60 | 80 | the half-lives of the enzyme at 60°C, 65°C, 70°C, 75°C, and 80°C are 142, 71, 35, 18, and 4.6 h, respectively | Caldicellulosiruptor saccharolyticus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.1.3.11 | 0.27 | - |
D-glucose | in 50 mM PIPES buffer (pH 7.5), at 75°C | Caldicellulosiruptor saccharolyticus | |
| 5.1.3.11 | 0.74 | - |
D-mannose | in 50 mM PIPES buffer (pH 7.5), at 75°C | Caldicellulosiruptor saccharolyticus | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.1.3.11 | 7.5 | - |
- |
Caldicellulosiruptor saccharolyticus |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.1.3.11 | 0.0038 | - |
D-glucose | in 50 mM PIPES buffer (pH 7.5), at 75°C | Caldicellulosiruptor saccharolyticus | |
| 5.1.3.11 | 0.0143 | - |
D-mannose | in 50 mM PIPES buffer (pH 7.5), at 75°C | Caldicellulosiruptor saccharolyticus | |
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