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Literature summary extracted from

  • Konno, N.; Sakamoto, Y.
    An endo-beta-1,6-glucanase involved in Lentinula edodes fruiting body autolysis (2011), Appl. Microbiol. Biotechnol., 91, 1365-1373.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.75 49000
-
 x * 49000, SDS-PAGE Lentinula edodes

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.75 Lentinula edodes F8WPI4
-
-
3.2.1.75 Lentinula edodes H 600 F8WPI4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.75 ammonium sulfate precipitation, Sepharose Q column chromatography, Superdex 75 gel filtration, and DEAE-Toyopearly column chromatography Lentinula edodes

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.75 fruit body
-
 Lentinula edodes
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.75 laminarin + H2O 3% activity compared to pustulan Lentinula edodes D-glucose + ? the main product is D-glucose ?
3.2.1.75 laminarin + H2O 3% activity compared to pustulan Lentinula edodes H 600 D-glucose + ? the main product is D-glucose ?
3.2.1.75 additional information no activity is detected when gentiobiose, lentinan, laminarioligosaccharides, pachyman, CM-pachyman, and lichenan are used as substrates Lentinula edodes ?
-
 ?
3.2.1.75 additional information the purified endo-beta-1,6-glucanase Pus30A catalyzes depolymerization of beta-1,6-glucan endolytically and is highly specific toward beta-1,6-glucan polysaccharide. Pus30A shows hydrolyzing activity against the cell wall components of Lentinula edodes fruiting bodies (54.5% activity compared to pustulan) Lentinula edodes ?
-
 ?
3.2.1.75 additional information no activity is detected when gentiobiose, lentinan, laminarioligosaccharides, pachyman, CM-pachyman, and lichenan are used as substrates Lentinula edodes H 600 ?
-
 ?
3.2.1.75 additional information the purified endo-beta-1,6-glucanase Pus30A catalyzes depolymerization of beta-1,6-glucan endolytically and is highly specific toward beta-1,6-glucan polysaccharide. Pus30A shows hydrolyzing activity against the cell wall components of Lentinula edodes fruiting bodies (54.5% activity compared to pustulan) Lentinula edodes H 600 ?
-
 ?
3.2.1.75 pustulan + H2O high substrate specificity, 100% activity Lentinula edodes ?
-
 ?
3.2.1.75 pustulan + H2O high substrate specificity, 100% activity Lentinula edodes H 600 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.75 ? x * 49000, SDS-PAGE Lentinula edodes

Synonyms

EC Number Synonyms Comment Organism
3.2.1.75 endo-beta-1,6-glucanase
-
 Lentinula edodes
3.2.1.75 Pus30A
-
 Lentinula edodes

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.75 50 60
-
 Lentinula edodes

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.75 60
-
 the enzyme is completely inactivated after incubation at 60°C for 30 min Lentinula edodes

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.75 4 5
-
 Lentinula edodes

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.75 4 8 the enzyme is stable over a broad pH range from pH 4.0 to 8.0 when treated at 4°C for 20 h Lentinula edodes

Expression

EC Number Organism Comment Expression
3.2.1.75 Lentinula edodes the transcript level of endo-beta-1,6-glucanase Pus30A gene (lepus30a) is significantly increased in fruiting bodies 4 days after harvesting (approximately 18times in gills, 27times in stipes, and 32times in pilei) compared to fresh fruiting bodies up

General Information

EC Number General Information Comment Organism
3.2.1.75 physiological function endo-beta-1,6-glucanase Pus30A is responsible for the degradation of the cell wall components during fruiting body autolysis after harvest Lentinula edodes