BRENDA - Enzyme Database

Unexpected property of ectoine synthase and its application for synthesis of the engineered compatible solute ADPC

Witt, E.M.; Davies, N.W.; Galinski, E.A.; Appl. Microbiol. Biotechnol. 91, 113-122 (2011)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
4.2.1.108
expression of His6-tagged ectoine synthase in Escherichia coli strain BL21(DE3)
Halomonas elongata
Engineering
EC Number
Protein Variants
Commentary
Organism
4.2.1.108
additional information
construction of enzyme-deficient strains by gene ectC inframe deletion though the splicing-by-overlap-extension PCR technique
Halomonas elongata
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.108
0.0287
-
L-homoectoine
recombinant enzyme, pH 8.5, 21C
Halomonas elongata
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.108
16520
-
x * 16520, recombinant His-tagged enzyme, SDS-PAGE
Halomonas elongata
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
4.2.1.108
N4-acetyl-L-2,4-diaminobutanoate
Halomonas elongata
an intramolecular condensation reaction
L-ectoine + H2O
-
-
r
4.2.1.108
N4-acetyl-L-2,4-diaminobutanoate
Halomonas elongata DSM 2581
an intramolecular condensation reaction
L-ectoine + H2O
-
-
r
Organism
EC Number
Organism
UniProt
Commentary
Textmining
4.2.1.108
Halomonas elongata
-
gene ectC
-
4.2.1.108
Halomonas elongata DSM 2581
-
gene ectC
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
4.2.1.108
recombinant His6-tagged ectoine synthase from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
Halomonas elongata
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
4.2.1.108
0.16
-
purified recombinant enzyme, substrate is 4,5-dihydro-2-methylimidazole-4-carboxylate, hydrolytic reaction, pH 8.5, 21C
Halomonas elongata
4.2.1.108
0.3
-
purified recombinant enzyme, substrate is 5-amino-3,4-dihydro-2H-pyrrole-2-carboxylate, hydrolytic reaction, pH 8.5, 21C
Halomonas elongata
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
4.2.1.108
D,L-4,5-dihydro-2-methylimidazole-4-carboxylate + H2O
-
713911
Halomonas elongata
?
-
-
-
r
4.2.1.108
D,L-4,5-dihydro-2-methylimidazole-4-carboxylate + H2O
-
713911
Halomonas elongata DSM 2581
?
-
-
-
r
4.2.1.108
L-glutamine
-
713911
Halomonas elongata
5-amino-3,4-dihydro-2H-pyrrole-2-carboxylate + H2O
-
-
-
r
4.2.1.108
L-glutamine
-
713911
Halomonas elongata DSM 2581
5-amino-3,4-dihydro-2H-pyrrole-2-carboxylate + H2O
-
-
-
r
4.2.1.108
L-homoectoine + H2O
-
713911
Halomonas elongata
?
-
-
-
r
4.2.1.108
additional information
ectoine synthase accepts more than one substrate and is a reversible enzyme able to catalyze both the intramolecular condensation into and the hydrolytic cleavage of cyclic amino acid derivatives. It forms 5-amino-3,4-dihydro-2H-pyrrole-2-carboxylate, ADPC, by cyclic condensation of glutamine in a reversible reaction. A number of ectoine derivatives, in particular 4,5-dihydro-2-methylimidazole-4-carboxylate and L-homoectoine, are also cleaved by ectoine synthase. Proposed reaction mechanism leading to L-ectoine and 5-amino-3,4-dihydro-2H-pyrrole-2-carboxylate, respectively, catalyzed by ectoine synthase, overview
713911
Halomonas elongata
?
-
-
-
-
4.2.1.108
additional information
ectoine synthase accepts more than one substrate and is a reversible enzyme able to catalyze both the intramolecular condensation into and the hydrolytic cleavage of cyclic amino acid derivatives. It forms 5-amino-3,4-dihydro-2H-pyrrole-2-carboxylate, ADPC, by cyclic condensation of glutamine in a reversible reaction. A number of ectoine derivatives, in particular 4,5-dihydro-2-methylimidazole-4-carboxylate and L-homoectoine, are also cleaved by ectoine synthase. Proposed reaction mechanism leading to L-ectoine and 5-amino-3,4-dihydro-2H-pyrrole-2-carboxylate, respectively, catalyzed by ectoine synthase, overview
713911
Halomonas elongata DSM 2581
?
-
-
-
-
4.2.1.108
N4-acetyl-L-2,4-diaminobutanoate
an intramolecular condensation reaction
713911
Halomonas elongata
L-ectoine + H2O
-
-
-
r
4.2.1.108
N4-acetyl-L-2,4-diaminobutanoate
an intramolecular condensation reaction
713911
Halomonas elongata
L-ectoine + H2O
i.e. 1,4,5,6-tetrahydro-2-methyl-4-pyrimidine carboxylic acid, over 95% conversion by the recombinant ectoine synthase
-
-
r
4.2.1.108
N4-acetyl-L-2,4-diaminobutanoate
an intramolecular condensation reaction
713911
Halomonas elongata DSM 2581
L-ectoine + H2O
-
-
-
r
4.2.1.108
N4-acetyl-L-2,4-diaminobutanoate
an intramolecular condensation reaction
713911
Halomonas elongata DSM 2581
L-ectoine + H2O
i.e. 1,4,5,6-tetrahydro-2-methyl-4-pyrimidine carboxylic acid, over 95% conversion by the recombinant ectoine synthase
-
-
r
Subunits
EC Number
Subunits
Commentary
Organism
4.2.1.108
?
x * 16520, recombinant His-tagged enzyme, SDS-PAGE
Halomonas elongata
Synonyms
EC Number
Synonyms
Commentary
Organism
4.2.1.108
EctC
-
Halomonas elongata
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
4.2.1.108
21
-
assay at
Halomonas elongata
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.108
8.5
-
assay at
Halomonas elongata
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.108
expression of His6-tagged ectoine synthase in Escherichia coli strain BL21(DE3)
Halomonas elongata
Engineering (protein specific)
EC Number
Protein Variants
Commentary
Organism
4.2.1.108
additional information
construction of enzyme-deficient strains by gene ectC inframe deletion though the splicing-by-overlap-extension PCR technique
Halomonas elongata
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.108
0.0287
-
L-homoectoine
recombinant enzyme, pH 8.5, 21C
Halomonas elongata
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.108
16520
-
x * 16520, recombinant His-tagged enzyme, SDS-PAGE
Halomonas elongata
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
4.2.1.108
N4-acetyl-L-2,4-diaminobutanoate
Halomonas elongata
an intramolecular condensation reaction
L-ectoine + H2O
-
-
r
4.2.1.108
N4-acetyl-L-2,4-diaminobutanoate
Halomonas elongata DSM 2581
an intramolecular condensation reaction
L-ectoine + H2O
-
-
r
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.108
recombinant His6-tagged ectoine synthase from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
Halomonas elongata
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
4.2.1.108
0.16
-
purified recombinant enzyme, substrate is 4,5-dihydro-2-methylimidazole-4-carboxylate, hydrolytic reaction, pH 8.5, 21C
Halomonas elongata
4.2.1.108
0.3
-
purified recombinant enzyme, substrate is 5-amino-3,4-dihydro-2H-pyrrole-2-carboxylate, hydrolytic reaction, pH 8.5, 21C
Halomonas elongata
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
4.2.1.108
D,L-4,5-dihydro-2-methylimidazole-4-carboxylate + H2O
-
713911
Halomonas elongata
?
-
-
-
r
4.2.1.108
D,L-4,5-dihydro-2-methylimidazole-4-carboxylate + H2O
-
713911
Halomonas elongata DSM 2581
?
-
-
-
r
4.2.1.108
L-glutamine
-
713911
Halomonas elongata
5-amino-3,4-dihydro-2H-pyrrole-2-carboxylate + H2O
-
-
-
r
4.2.1.108
L-glutamine
-
713911
Halomonas elongata DSM 2581
5-amino-3,4-dihydro-2H-pyrrole-2-carboxylate + H2O
-
-
-
r
4.2.1.108
L-homoectoine + H2O
-
713911
Halomonas elongata
?
-
-
-
r
4.2.1.108
additional information
ectoine synthase accepts more than one substrate and is a reversible enzyme able to catalyze both the intramolecular condensation into and the hydrolytic cleavage of cyclic amino acid derivatives. It forms 5-amino-3,4-dihydro-2H-pyrrole-2-carboxylate, ADPC, by cyclic condensation of glutamine in a reversible reaction. A number of ectoine derivatives, in particular 4,5-dihydro-2-methylimidazole-4-carboxylate and L-homoectoine, are also cleaved by ectoine synthase. Proposed reaction mechanism leading to L-ectoine and 5-amino-3,4-dihydro-2H-pyrrole-2-carboxylate, respectively, catalyzed by ectoine synthase, overview
713911
Halomonas elongata
?
-
-
-
-
4.2.1.108
additional information
ectoine synthase accepts more than one substrate and is a reversible enzyme able to catalyze both the intramolecular condensation into and the hydrolytic cleavage of cyclic amino acid derivatives. It forms 5-amino-3,4-dihydro-2H-pyrrole-2-carboxylate, ADPC, by cyclic condensation of glutamine in a reversible reaction. A number of ectoine derivatives, in particular 4,5-dihydro-2-methylimidazole-4-carboxylate and L-homoectoine, are also cleaved by ectoine synthase. Proposed reaction mechanism leading to L-ectoine and 5-amino-3,4-dihydro-2H-pyrrole-2-carboxylate, respectively, catalyzed by ectoine synthase, overview
713911
Halomonas elongata DSM 2581
?
-
-
-
-
4.2.1.108
N4-acetyl-L-2,4-diaminobutanoate
an intramolecular condensation reaction
713911
Halomonas elongata
L-ectoine + H2O
-
-
-
r
4.2.1.108
N4-acetyl-L-2,4-diaminobutanoate
an intramolecular condensation reaction
713911
Halomonas elongata
L-ectoine + H2O
i.e. 1,4,5,6-tetrahydro-2-methyl-4-pyrimidine carboxylic acid, over 95% conversion by the recombinant ectoine synthase
-
-
r
4.2.1.108
N4-acetyl-L-2,4-diaminobutanoate
an intramolecular condensation reaction
713911
Halomonas elongata DSM 2581
L-ectoine + H2O
-
-
-
r
4.2.1.108
N4-acetyl-L-2,4-diaminobutanoate
an intramolecular condensation reaction
713911
Halomonas elongata DSM 2581
L-ectoine + H2O
i.e. 1,4,5,6-tetrahydro-2-methyl-4-pyrimidine carboxylic acid, over 95% conversion by the recombinant ectoine synthase
-
-
r
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.2.1.108
?
x * 16520, recombinant His-tagged enzyme, SDS-PAGE
Halomonas elongata
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
4.2.1.108
21
-
assay at
Halomonas elongata
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.108
8.5
-
assay at
Halomonas elongata
General Information
EC Number
General Information
Commentary
Organism
4.2.1.108
malfunction
in Halomonas elongata mutant strains deficient in ectA, encoding L-2,4-diaminobutyric acid acetyltransferase, no ectoine synthesis is obeserved
Halomonas elongata
4.2.1.108
metabolism
ectoine synthase is involved in the biosynthetic pathway of L-ectoine and (S,S)-beta-hydroxyectoine, involvement of ectoine synthase in 5-amino-3,4-dihydro-2H-pyrrole-2-carboxylate biosynthesis in vitro
Halomonas elongata
4.2.1.108
physiological function
extraordinary protective properties of 5-amino-3,4-dihydro-2H-pyrrole-2-carboxylate produced by ectoine synthase
Halomonas elongata
General Information (protein specific)
EC Number
General Information
Commentary
Organism
4.2.1.108
malfunction
in Halomonas elongata mutant strains deficient in ectA, encoding L-2,4-diaminobutyric acid acetyltransferase, no ectoine synthesis is obeserved
Halomonas elongata
4.2.1.108
metabolism
ectoine synthase is involved in the biosynthetic pathway of L-ectoine and (S,S)-beta-hydroxyectoine, involvement of ectoine synthase in 5-amino-3,4-dihydro-2H-pyrrole-2-carboxylate biosynthesis in vitro
Halomonas elongata
4.2.1.108
physiological function
extraordinary protective properties of 5-amino-3,4-dihydro-2H-pyrrole-2-carboxylate produced by ectoine synthase
Halomonas elongata